Distance to the water table shapes the diversity and activity of DNA and RNA viruses in a subalpine peatland.

Peatlands are essential reservoirs of carbon and critical zones for the cycling of greenhouse gases on Earth. Their ecological functions are primarily governed by the microbial communities inhabiting them, which vary with hydrological conditions. However, the roles of viruses in peatland ecosystems remain poorly understood despite their abundance and ubiquity.

Considerations and recommendations from the ISMRM diffusion study group for preclinical diffusion MRI: Part 2-Ex vivo imaging: Added value and acquisition.

The value of preclinical diffusion MRI (dMRI) is substantial. While dMRI enables in vivo non-invasive characterization of tissue, ex vivo dMRI is increasingly being used to probe tissue microstructure and brain connectivity. Ex vivo dMRI has several experimental advantages including higher SNR and spatial resolution compared to in vivo studies, and enabling more advanced diffusion contrasts for improved microstructure and connectivity characterization.

Structured Doubly Stochastic Graph-Based Clustering.

Graph-based clustering is a hot topic in machine learning, whose effectiveness highly relies on the quality of the learned graph. Recent researches preferred to learn the nearest doubly stochastic approximation of a graph to suppress intercluster connections and enhance intracluster connections and thus improve clustering performance. While current paradigm is limited by three key problems: 1) it is restricted by a predefined graph; 2) the separated stages of spectral decomposition-based way (graph learning, spectral embedding learning, and cluster assignment by k-means) cause mismatched problems and randomness; and 3) the optimization of doubly stochastic conditions is generally achieved by von Neumann successive projection (VNSP) lemma, which separates the conditions to form two subproblems for alternative optimization, converging only to a feasible solution.

Considerations and recommendations from the ISMRM diffusion study group for preclinical diffusion MRI: Part 1: In vivo small-animal imaging.

Small-animal diffusion MRI (dMRI) has been used for methodological development and validation, characterizing the biological basis of diffusion phenomena, and comparative anatomy. The steps from animal setup and monitoring, to acquisition, analysis, and interpretation are complex, with many decisions that may ultimately affect what questions can be answered using the resultant data. This work aims to present selected considerations and recommendations from the diffusion community on best practices for preclinical dMRI of in vivo animals.

Considerations and recommendations from the ISMRM Diffusion Study Group for preclinical diffusion MRI: Part 3-Ex vivo imaging: Data processing, comparisons with microscopy, and tractography.

Preclinical diffusion MRI (dMRI) has proven value in methods development and validation, characterizing the biological basis of diffusion phenomena, and comparative anatomy. While dMRI enables in vivo non-invasive characterization of tissue, ex vivo dMRI is increasingly being used to probe tissue microstructure and brain connectivity. Ex vivo dMRI has several experimental advantages that facilitate high spatial resolution and high SNR images, cutting-edge diffusion contrasts, and direct comparison with histological data as a methodological validation.

NAC transcription factor GbNTL9 modifies the accumulation and organization of cellulose microfibrils to enhance cotton fiber strength.

Introduction: Fiber strength is a critical determinant of fiber quality, with stronger fibers being highly preferred in the cotton textile industry. However, the genetic basis and the specific regulatory mechanism underlying the formation of cotton fiber strength remain largely unknown.

Objectives: To explore fiber strength-related genes, QTL mapping, map-based cloning, and gene function verification were conducted in a backcross inbred line BS41 derived from interspecific hybridization between upland cotton and sea-island cotton.

[Preliminary exploration of multi-omics data fusion methods for high-dimensional small-sample datasets in traditional Chinese medicine].

With the advancement in big data and artificial intelligence technologies, the extensive application of omics technologies in traditional Chinese medicine(TCM) research has generated large experimental datasets, enabling the exploration of cross-scale correlations among massive data and thereby resulting in the shift toward a data-intensive research paradigm. The emerging approach of multi-omics data fusion analysis, emphasizing technical and computational tools, presents a potential breakthrough in this field. The holistic perspective of TCM aligns with the concept of multi-omics data fusion, yet the data types encountered exhibit high dimensionality with small sample sizes, necessitating data processing techniques such as dimensionality reduction.

Functional Characterization of Transcriptional Regulator Rem in Liberibacter asiaticus.

Citrus HLB, caused by Liberibacter asiaticus (CLas), is the most devastating citrus disease worldwide. Usually 10% of total genes in bacteria are regulatory genes, but only 2% of CLas genes encode transcriptional factors. Here, 20 transcriptional regulators were predicted including eight genes ( and ) directly or indirectly involved in regulating motility, and five genes (i.

Exploration of the Interactions Between subsp. --Citrus to Improve -Mediated Transient Expression in Plants.

-mediated transient expression (AMTE) is an important tool in plant genetics study and biotechnology. AMTE remains problematic in citrus and many plant species. Previous study has shown that pretreatment of citrus leaves with subsp.

How does digital literacy affect the health status of senior citizens? Micro-level evidence from the CFPS data.

Background: The popularization of the Internet and digital technology has called for higher digital literacy among citizens, especially the elderly. However, most existing studies didn't measure digital literacy at the micro level, and the impact mechanism has rarely been discussed. The purpose of this study is to clarify whether and how digital literacy affects the health status of senior citizens.

Integrated Transcriptome and Metabolome Analysis Reveals Insights into Flavone and Flavonol Biosynthesis in Salicylic Acid-Induced Huanglongbing Tolerance.

Salicylic acid (SA) exhibits positive effects against Huanglongbing (HLB), but how SA affects citrus resistance to HLB is currently unknown. This study conducted integrated transcriptome and metabolome analyses on SA-treated (HLB-sensitive) and (HLB-tolerant). The results indicated that the syntheses of flavones and flavonols were induced by SA, while the expression levels of associated genes and the contents of corresponding metabolites varied significantly between the two species after SA treatment or HLB infection.

Regulated cell death in acute myocardial infarction: Molecular mechanisms and therapeutic implications.

Acute myocardial infarction (AMI), primarily caused by coronary atherosclerosis, initiates a series of events that culminate in the obstruction of coronary arteries, resulting in severe myocardial ischemia and hypoxia. The subsequent myocardial ischemia/reperfusion (I/R) injury further aggravates cardiac damage, leading to a decline in heart function and the risk of life-threatening complications. The complex interplay of multiple regulated cell death (RCD) pathways plays a pivotal role in the pathogenesis of AMI.

Nerve-Inspired Optical Waveguide Stretchable Sensor Fusing Wireless Transmission and AI Enabling Smart Tele-Healthcare.

Flexible strain monitoring of hand and joint muscle movement is recognized as an effective method for the diagnosis and rehabilitation of neurological diseases such as stroke and Parkinson's disease. However, balancing high sensitivity and large strain, improving wearing comfort, and solving the separation of diagnosis and treatment are important challenges for further building tele-healthcare systems. Herein, a hydrogel-based optical waveguide stretchable (HOWS) sensor is proposed in this paper.

Microbial community of cultivated and uncultivated citrus rhizosphere microbiota in Brazil.

The rhizosphere microbiome is known to contain beneficial microorganisms that promote plant growth and increase tolerance to abiotic and biotic stresses. Understanding citrus microbiome diversity and the percentage of diversity that can be recovered in the laboratory is essential for developing innovative approaches to improve plant health and promote sustainable agricultural practices. However, information about the citrus root microbiome, especially in the context of exploring commercial citrus growing areas to identify beneficial plant growth-promoting rhizobacteria (PGPR), is scarce.

Haplotype-resolved genome assembly of poplar line NL895 provides a valuable tree genomic resource.

Poplar line NL895 can potentially become a model plant for poplar study as it is a widely cultivated elite line. However, the lack of genome resources hindered the use of NL895 as the major plant material in poplar. In this study, we provided a high-quality genome assembly for poplar line NL895 with PacBio single molecule real-time (SMRT) sequencing and High-throughput chromosome conformation capture (Hi-C) technology.

TRANSPARENT TESTA 16 collaborates with the MYB-bHLH-WD40 transcriptional complex to produce brown fiber cotton.

Naturally colored cotton (NCC; Gossypium spp.) does not require additional chemical dyeing and is an environmentally friendly textile material with great research potential and applications. Our previous study using linkage and association mapping identified TRANSPARENT TESTA 2 (Gh_TT2) as acting on the proanthocyanin synthesis pathway.

Mass spectrometry unveils heat-induced changes in yolk oxylipins and key lipid molecules during home cooking.

Introduction: Oxylipins, as a widespread class of metabolic markers following oxidative stress, and several studies have reported dietary regulation of lipid metabolism. However, there is a lack of investigation of dietary oxylipins, especially cooking-induced changes in food lipid oxidation.

Objectives: Investigated the effects of cooking methods and lipid profiles on polyunsaturated fatty acids derived oxylipins generation within egg yolks.

Up-regulation of MDSCs accumulation and Th2 biased response to co-stimulation of CsESP from Clonorchis sinensis and HBeAg in vitro.

Co-infection with Clonorchis sinensis (C. sinensis) and Hepatitis B virus (HBV) are commonly observed in endemic areas of Clonorchiasis. Chronic infection of C.

SDE19, a SEC-dependent effector from 'Candidatus Liberibacter asiaticus' suppresses plant immunity and targets Citrus sinensis Sec12 to interfere with vesicle trafficking.

Citrus huanglongbing (HLB), which is caused by the phloem-colonizing bacteria Candidatus Liberibacter asiaticus (CLas), poses a significant threat to citrus production worldwide. The pathogenicity mechanism of HLB remains poorly understood. SEC-dependent effectors (SDEs) have been suggested to play critical roles in the interaction between citrus and CLas.

Pleural parasite infection presenting with an isolated pleural effusion misdiagnosed as tuberculosis: a case report.

Pleural parasitic infection is an extremely rare disease of the pleura caused by a variety of parasites, with paragonimiasis infection being the most common. The lack of specific clinical symptoms for paragonimiasis makes it easy to misdiagnose as tuberculosis, causing unnecessary drug-related adverse effects and financial burdens from incorrect treatment. We report a case of a pediatric patient presenting with an isolated pleural effusion that was misdiagnosed as tuberculosis; the patient was eventually diagnosed with pleuropulmonary paragonimiasis infection after immunologic and serologic tests.

Overlooked role of CO· reactivity with different dissociation forms of organic micropollutants in degradation kinetics modeling: A case study of fluoxetine degradation in a UV/peroxymonosulfate system.

Selective oxidizing agent carbonate radical (CO) is an important secondary radical in radical-based advanced oxidation technology for wastewater treatment. However, the role of CO in removing ionizable organic micropollutants (OMs) under environmentally relevant conditions remains unclear. Herein we investigated CO effect on degradation kinetics of fluoxetine in UV/peroxymonosulfate (PMS) system based on a built radical model considering CO reactivity differences with its different dissociation forms.

Cas12a RNP-mediated co-transformation enables transgene-free multiplex genome editing, long deletions, and inversions in citrus chromosome.

Introduction: Citrus canker, caused by subsp. citri (), is a devastating disease worldwide. Previously, we successfully generated canker-resistant cv.

Global citrus root microbiota unravels assembly cues and core members.

Introduction: Citrus is one of the most important fruit crops worldwide, and the root-associated microbiota can have a profound impact on tree health and growth.

Methods: In a collaborative effort, the International Citrus Microbiome Consortium investigated the global citrus root microbiota with samples collected from nine citrus-producing countries across six continents. We analyzed 16S rDNA and ITS2 amplicon sequencing data to identify predominant prokaryotic and fungal taxa in citrus root samples.