Publications by authors named "Ni-na Yang"

Bemisia tabaci has developed a high level of resistance to thiamethoxam, a second generation neonicotinoid insecticide that has been widely used to control this pest. In this study, we investigated whether hydroxyacid-oxoacid transhydrogenase (HOT) is involved in resistance to the neonicotinoid insecticide thiamethoxam in the whitefly. We cloned the full-length gene that encodes HOT in B.

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LpGPAT was obtained from L. pensylvanicum using RT-PCR and rapid amplification of cDNA ends. The cloned full-length cDNA was 1544 bp; it encoded 410 amino acids and had a molecular size of 46 KDa.

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The sweet potato whitefly, Bemisia tabaci (Gennadius) (Hemiptera:Aleyrodidae), is an invasive and damaging pest of field crops worldwide. The neonicotinoid insecticide imidacloprid has been widely used to control this pest. We assessed the species composition (B vs.

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Article Synopsis
  • Thiamethoxam, a widely used insecticide for controlling sweetpotato whiteflies, is facing resistance due to excessive use, leading to research on the molecular mechanisms of this resistance.
  • Researchers employed suppression subtractive hybridization (SSH) to compare gene profiles between resistant and susceptible whitefly strains, identifying a total of 124 differentially expressed genes categorized by various functions.
  • Validation through quantitative real-time PCR (qRT-PCR) found that while there were consistent results, only about 50% of the genes displayed significant expression differences, with one notable gene, a NAD-dependent methanol dehydrogenase, being highly over-expressed in resistant strains, particularly during the egg stage and in adult females' abdomens
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Background: Bemisia tabaci (Gennadius) is a phloem-feeding insect poised to become one of the major insect pests in open field and greenhouse production systems throughout the world. The high level of resistance to insecticides is a main factor that hinders continued use of insecticides for suppression of B. tabaci.

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