Construction and functional verification of size-reduced plasmids based on TMP resistance gene .

Plasmid size is one of the factors affecting transfection efficacy in most of the molecular genetic research studies. One effective approach for reducing plasmid size is to replace relatively large, conventional antibiotic resistance genes with the short-size gene. The successful construct of a series of based tool plasmids and their functional validation, via comparison with original plasmids, suggest that is a potent drug resistance selection marker.