Programmable Acetylation Modification of Bacterial Proteins by a Cas12a-Guided Acetyltransferase.

Protein lysine acetylation (PLA) is a crucial post-translational modification in organisms that regulates a variety of metabolic and physiological activities. Many advances have been made in PLA-related research; however, the quick and accurate identification of causal relationships between specific protein acetylation events and phenotypic outcomes at the proteome level remains challenging due to the lack of efficient targeted modification techniques. In this study, based on the characteristics of transcription-translation coupling in bacteria, we designed and constructed an in situ targeted protein acetylation (TPA) system integrating the dCas12a protein, guiding element crRNA, and bacterial acetylase At2.

Fungal CFEM effectors negatively regulate a maize wall-associated kinase by interacting with its alternatively spliced variant to dampen resistance.

The fungus Fusarium graminearum causes a devastating disease Gibberella stalk rot of maize. Our knowledge of molecular interactions between F. graminearum effectors and maize immunity factors is lacking.

The complete mitochondrial genome of the Daimio tethys (Lepidoptera: Hesperoidea: Hesperiidae).

The complete mitochondrial genome of Daimio tethys (Hesperoidea: Hesperiidae) is a circular molecule of 15,341 bp in length, containing 37 typical animal mitochondrial genes: 13 protein coding genes (ATP6, ATP8, COI-III, ND1-6, ND4L, Cytb), 2 rRNA genes, 22 tRNA genes and a non-coding AT-rich region. Its gene order and content are identical to all other available butterfly mitogenomes. All PCGs initiate with typical ATN codons, except for COI, which is initiated by the CGA codon as observed in other butterfly species.

The complete mitochondrial genome of Tirumala limniace (Lepidoptera: Nymphalidae: Danainae).

The complete mitochondrial nucleotide sequence of Tirumala limniace is 15,285 bp in size, containing typical metazoan 37 genes: 13 protein-coding genes (PCGs), 22tRNA genes, 2 ribosomal RNA genes and one noncoding AT-rich region. All the 13 PCGs initiated with ATN, except for COI gene which is started by CGA codon. Nine PCGs use the complete termination codon (TAA), whereas the COI, COII, ND4 and ND5 genes end with single T.

The complete mitochondrial genome of the Byasa alcinous (Lepidoptera: Papilionidae: Papilioninae).

The complete mitochondrial genome (mitogenome) of Byasa alcinous (Lepidoptera: Papilionidae: Papilioninae) is a circular molecule of 15,266 bp in length, containing 37 typical insect mitochondrial genes: 13 protein coding genes (PCGs), 2 rRNA genes, 22 tRNA genes and a non-coding AT-rich region. Its gene order and arrangement are identical to all other available butterfly mitogenomes. All PCGs start with a typical ATN initiation codon, except for COI, which is initiated by the CGA codon as observed in other butterfly species.

The complete mitochondrial genome of Triphysa phryne (Lepidoptera: Nymphalidae: Satyrinae).

The complete mitochondrial genome (mitogenome) sequence of Triphysa phryne (Lepidoptera: Nymphalidae: Satyrinae) was determined in this study. The mitogenome is 15,143 bp in length, containing 37 typical animal mitochondrial genes: 13 putative protein-coding genes (PCGs), 2 ribosomal RNAs, 22 transfer RNAs and a non-coding AT-rich region. Its gene content and order are identical to those of other lepidopteran mitogenomes.

The complete mitochondrial genome of Ideopsis similis (Lepidoptera: Nymphalidae: Danainae).

The complete mitochondrial nucleotide sequence of Ideopsis similis is 15,200 bp in size, containing typical metazoan 37 genes: 13 protein-coding genes (PCGs), 22 tRNA genes, 2 ribosomal RNA genes and 1 non-coding AT-rich region. All the 13 PCGs initiated with ATN, except for COI gene which started by CGA codon. Nine PCGs used the complete termination codon (TAA), whereas the COI, COII, ND4 and ND5 genes ended with single T.