Prokaryotic communities profiling of Indonesian hot springs using long-read Oxford Nanopore sequencing.

Objectives: Indonesia's location at the convergence of multiple tectonic plates results in a unique geomorphological feature with abundant hot springs. This study pioneers the metagenomic exploration of Indonesian hot springs, harbouring unique life forms despite high temperatures. The microbial community of hot springs is taxonomically versatile and biotechnologically valuable.

Indonesian marine and its medicinal contribution.

The archipelagic country of Indonesia is populated by the densest marine biodiversity in the world which has created strong global interest and is valued by both Indigenous and European settlements for different purposes. Nearly 1000 chemicals have been extracted and identified. In this review, a systematic data curation was employed to collate bioprospecting related manuscripts providing a comprehensive directory based on publications from 1988 to 2022.

Phenanthroindolizidine Alkaloids Isolated from as Potent Inhibitors of Inflammation, Spheroid Growth, and Invasion of Triple-Negative Breast Cancer.

Triple-negative breast cancer (TNBC), representing the most aggressive form of breast cancer with currently no targeted therapy available, is characterized by an inflammatory and hypoxic tumor microenvironment. To date, a broad spectrum of anti-tumor activities has been reported for phenanthroindolizidine alkaloids (PAs), however, their mode of action in TNBC remains elusive. Thus, we investigated six naturally occurring PAs extracted from the plant : -methyltylophorinidine () and its five derivatives tylophorinidine (), tylophoridicine E (), 2-demethoxytylophorine (), tylophoridicine D (), and anhydrodehydrotylophorinidine ().

Apoptotic Activity of New Oxisterigmatocystin Derivatives from the Marine-Derived Fungus NC06.

Sponge-derived fungi have recently attracted attention as an important source of interesting bioactive compounds. NC06 was isolated from the marine sponge . This fungus was cultured on rice medium and yielded four compounds including three new oxisterigmatocystins, namely, J, K, and L (, , and ), and one known compound, aspergillicin A ().

Pseudopterosin and -Methyltylophorinidine Suppress Cell Growth in a 3D Spheroid Co-Culture Model of Pancreatic Ductal Adenocarcinoma.

Current therapies for treating pancreatic ductal adenocarcinoma (PDAC) are largely ineffective, with the desmoplastic environment established within these tumors being considered a central issue. We established a 3D spheroid co-culture in vitro model using a PDAC cell line (either PANC-1 or Capan-2), combined with stellate cells freshly isolated from pancreatic tumors (PSC) or hepatic lesions (HSC), and human type I collagen to analyze the efficiency of the chemotherapeutic gemcitabine (GEM) as well as two novel drug candidates derived from natural products: pseudopterosin (PsA-D) and -methyltylophorinidine (TYLO). Traditional 2D in vitro testing of these agents for cytotoxicity on PANC-1 demonstrated IC values of 4.

Didymellanosine, a new decahydrofluorene analogue, and ascolactone C from sp. IEA-3B.1, an endophyte of .

Didymellanosine (1), the first analogue of the decahydrofluorene-class of natural products bearing a 13-membered macrocyclic alkaloid conjugated with adenosine, and a new benzolactone derivative, ascolactone C (4) along with eight known compounds (2, 3, 5-10), were isolated from a solid rice fermentation of the endophytic fungus sp. IEA-3B.1 derived from the host plant .

Expanding the chemical diversity of an endophytic fungus , an ascomycete associated with mistletoe, through an OSMAC approach.

An endophytic fungus (isolate MSp3-1), isolated from mistletoe (), was subjected to fermentation on solid Czapek medium. Chromatographic workup of the crude EtOAc extract yielded five new natural products (1-5). Subsequent application of the "One Strain, MAny Compounds" (OSMAC) strategy on this strain by the addition of a mixture of salts (MgSO, NaNO and NaCl) to solid Czapek medium induced the accumulation of nine additional new secondary metabolites (6-13, 16), with most of them (8, 10-12) not detectable in cultures lacking the salt mixture.

Indole Diterpenoids from an Endophytic Penicillium sp.

A chemical investigation of the endophyte Penicillium sp. (strain ZO-R1-1), isolated from roots of the medicinal plant Zingiber officinale, yielded nine new indole diterpenoids (1-9), together with 13 known congeners (10-22). The structures of the new compounds were elucidated by 1D and 2D NMR analysis in combination with HRESIMS data.