Manufacturing exosomes for wound healing: Comparative analysis of culture media.

Mesenchymal stem cell (MSC)-derived exosomes (EXs) have emerged as promising therapeutic agents for wound healing. However, the optimal conditions for manufacturing MSC-derived EXs that maximize their wound-healing potential have yet to be established. Hence, we compared the efficacy of five different MSC culture media, including three different serum-free, a platelet-supplemented, and a fetal bovine serum-supplemented media, in exosome manufacturing for wound healing applications.

Integration of estimated regional gene expression with neuroimaging and clinical phenotypes at biobank scale.

An understanding of human brain individuality requires the integration of data on brain organization across people and brain regions, molecular and systems scales, as well as healthy and clinical states. Here, we help advance this understanding by leveraging methods from computational genomics to integrate large-scale genomic, transcriptomic, neuroimaging, and electronic-health record data sets. We estimated genetically regulated gene expression (gr-expression) of 18,647 genes, across 10 cortical and subcortical regions of 45,549 people from the UK Biobank.

Rifampicin tolerance and growth fitness among isoniazid-resistant clinical isolates from a longitudinal study.

Antibiotic tolerance in reduces bacterial killing, worsens treatment outcomes, and contributes to resistance. We studied rifampicin tolerance in isolates with or without isoniazid resistance (IR). Using a minimum duration of killing assay, we measured rifampicin survival in isoniazid-susceptible (IS, n=119) and resistant (IR, n=84) isolates, correlating tolerance with bacterial growth, rifampicin minimum inhibitory concentrations (MICs), and isoniazid-resistant mutations.

Co-design personal sleep health technology for and with university students.

University students often experience sleep disturbances and disorders. Personal digital technologies present a great opportunity for sleep health promotion targeting this population. However, studies that engage university students in designing and implementing digital sleep health technologies are scarce.

Rifampicin tolerance and growth fitness among isoniazid-resistant clinical isolates: an in-vitro longitudinal study.

Antibiotic tolerance in leads to less effective bacterial killing, poor treatment responses and resistant emergence. Therefore, we investigated the rifampicin tolerance of isolates, with or without pre-existing isoniazid-resistance. We determined the rifampicin survival fraction by minimum duration of killing assay in isoniazid susceptible (IS, n=119) and resistant (IR, n=84) isolates.

Pollution characteristics, associated risks, and possible sources of heavy metals in road dust collected from different areas of a metropolis in Vietnam.

Road dust samples were collected from different areas in Ho Chi Minh City (HCMC)-the largest city in Vietnam to explore pollution characteristics, ecological and human health risks, and sources of heavy metals (HMs). Results revealed the level of HMs found in the samples from residential and industrial zones of HCMC in the order of Mn > Zn > Cu > Cr > Pb > Ni > Co > As > Cd, Zn > Mn > Cu > Cr > Pb > Ni > Co > As > Cd. Due to the high enrichment of Cu, Zn in residential areas and Cu, Pb, Zn in industrial areas, the HM contamination in these areas remained moderate to severe.

Knowledge Discovery in Ubiquitous and Personal Sleep Tracking: Scoping Review.

Background: Over the past few decades, there has been a rapid increase in the number of wearable sleep trackers and mobile apps in the consumer market. Consumer sleep tracking technologies allow users to track sleep quality in naturalistic environments. In addition to tracking sleep per se, some sleep tracking technologies also support users in collecting information on their daily habits and sleep environments and reflecting on how those factors may contribute to sleep quality.

The Correlation between Peripheral Blood Index and Immune Cell Expansion in Vietnamese Elderly Lung Cancer Patients.

(1) Background: The dysfunction and reduced proliferation of peripheral CD8 T cells and natural killer (NK) cells have been observed in both aging and cancer patients, thereby challenging the adoption of immune cell therapy in these subjects. In this study, we evaluated the growth of these lymphocytes in elderly cancer patients and the correlation of peripheral blood (PB) indices to their expansion. (2) Method: This retrospective study included 15 lung cancer patients who underwent autologous NK cell and CD8 T cell therapy between January 2016 and December 2019 and 10 healthy individuals.

The density of bone marrow mononuclear cells and CD34+ cells in patients with three neurologic conditions.

Background: This study aimed to identify the density of mononuclear cells (MNCs) and CD34+ cells in the bone marrow of patients with three neurologic conditions.

Methods: The study included 88 patients with three neurologic conditions: 40 with cerebral palsy (CP) due to oxygen deprivation (OD), 23 with CP related to neonatal icterus (NI), and 25 with neurological sequelae after traumatic brain injury. Bone marrow aspiration was conducted from the patients' bilateral anterior iliac crest under general anesthesia in an operating theater.

Differential Cytotoxicity of Curcumin-Loaded Micelles on Human Tumor and Stromal Cells.

Although curcumin in the form of nanoparticles has been demonstrated as a potential anti-tumor compound, the impact of curcumin and nanocurcumin in vitro on normal cells and in vivo in animal models is largely unknown. This study evaluated the toxicity of curcumin-loaded micelles in vitro and in vivo on several tumor cell lines, primary stromal cells, and zebrafish embryos. Breast tumor cell line (MCF7) and stromal cells (human umbilical cord vein endothelial cells, human fibroblasts, and human umbilical cord-derived mesenchymal stem cells) were used in this study.

Management factors influencing lecturers' research productivity in Vietnam National University, Hanoi, Vietnam: A structural equation modeling analysis.

Increasing lecturers' research productivity plays a crucial role in improving teaching quality and university prestige. This research aims to establish and test a model for evaluating management factors that affect the research productivity of Vietnamese university lecturers. Data were collected by surveying 398 lecturers and researchers at universities affiliated with the Vietnam National University, Hanoi (VNU).

Exploring the Kinh Vietnamese genomic database for the polymorphisms of the P450 genes towards precision public health.

Background: Human cytochrome P450 (s) genes are essential in metabolising drugs. Due to their high polymorphism, population-specific studies are of great interest.

Aim: This research examined the six genes, including , , , , , and in the Kinh Vietnamese (KHV) for population-scale precision medicine.

Plastic waste as a valuable resource: strategy to remove heavy metals from wastewater in bench scale application.

Single-use plastic waste is gradually considered a potential material for circular economy. Ion exchange resin obtained from polystyrene waste by sulfonating with HSO was used for heavy metal removal from electroplating wastewater. Batch mode experiments of Cu, Zn, and Cd were carried out to determine effect of pH, initial concentration, equilibrium time, and the isotherm and kinetic parameters; the stability of the resin in continuous operation was then evaluated.

Characteristics of polycyclic aromatic hydrocarbons in ambient air of a tropical mega-area, Ho Chi Minh City, Vietnam: concentration, distribution, gas/particle partitioning, potential sources and cancer risk assessment.

This is the first investigation on overall characteristics of 25 polycyclic aromatic hydrocarbons (PAHs) (15 PAHs regulated by US-EPA (excluding naphthalene) and 16 PAHs recommended by the European Union) in ambient air of Ho Chi Minh City, Vietnam. Their levels, congener profiles, gas/particle partitioning, potential sources of atmospheric PAHs (gas and particulate phases), and lung cancer risks in the dry and rainy seasons were examined. The ∑25 PAH concentration in the dry and rainy seasons ranged from 8.

Optimization of human umbilical cord blood-derived mesenchymal stem cell isolation and culture methods in serum- and xeno-free conditions.

Background: Although umbilical cord blood (UCB) is identified as a source of mesenchymal stem cells (MSCs) with various advantages, the success in cell isolation is volatile. Therefore, it is necessary to optimize methods of cord blood-derived MSC (UCB-MSC) isolation and culture. In this study, we evaluated the efficiency of UCB-MSC isolation and expansion using different commercially available serum- and xeno-free media and investigated the capacity of autologous serum and plasma as a supplement to support cell proliferation.

Automatic inference of demographic parameters using generative adversarial networks.

Population genetics relies heavily on simulated data for validation, inference and intuition. In particular, since the evolutionary 'ground truth' for real data is always limited, simulated data are crucial for training supervised machine learning methods. Simulation software can accurately model evolutionary processes but requires many hand-selected input parameters.

Most-Probable-Number-Based Minimum Duration of Killing Assay for Determining the Spectrum of Rifampicin Susceptibility in Clinical Mycobacterium tuberculosis Isolates.

Accurate antibiotic susceptibility testing is essential for successful tuberculosis treatment. Recent studies have highlighted the limitations of MIC-based phenotypic susceptibility methods in detecting other aspects of antibiotic susceptibilities in bacteria. Duration and peak of antibiotic exposure, at or above the MIC required for killing the bacterial population, has emerged as another important factor for determining antibiotic susceptibility.

Vγ9γδ T Cell Induction by Human Umbilical Cord Blood Monocytes-Derived, Interferon-α-Stimulated Dendritic Cells.

Dendritic cells (DC) are professional antigen-presenting cells that activate T cells to kill cancer cells. The extracellular products of DCs have also been reported to perform the same function. In this study, we examined the differentiation of umbilical cord blood monocytes into DCs in the presence of GM-CSF, and interferon (IFN)-α.

Variations in Antimicrobial Activities of Human Monocyte-Derived Macrophage and Their Associations With Tuberculosis Clinical Manifestations.

Macrophages play a significant role in preventing infection through antimicrobial activities, particularly acidification, and proteolysis. infection can lead to diverse outcomes, from latent asymptomatic infection to active disease involving multiple organs. Monocyte-derived macrophage is one of the main cell types accumulating in lungs following infection.

Status of water use and potential of rainwater harvesting for replacing centralized supply system in remote mountainous areas: a case study.

The failure of the centralized water supply system forced XY community to become more dependent on uncertain and unstable water sources. The results of surveying 50 households showed that 89.18% of total households depended on water collected from rivers, which contributed 58.

Differential Wound Healing Capacity of Mesenchymal Stem Cell-Derived Exosomes Originated From Bone Marrow, Adipose Tissue and Umbilical Cord Under Serum- and Xeno-Free Condition.

Exosomes are nano-scale and closed membrane vesicles which are promising for therapeutic applications due to exosome-enclosed therapeutic molecules such as DNA, small RNAs, proteins and lipids. Recently, it has been demonstrated that mesenchymal stem cell (MSC)-derived exosomes have capacity to regulate many biological events associated with wound healing process, such as cell proliferation, cell migration and blood vessel formation. This study investigated the regenerative potentials for cutaneous tissue, in regard to growth factors associated with wound healing and skin cell proliferation and migration, by exosomes released from primary MSCs originated from bone marrow (BM), adipose tissue (AD), and umbilical cord (UC) under serum- and xeno-free condition.

The role of microRNAs in the legume-Rhizobium nitrogen-fixing symbiosis.

Under nitrogen starvation, most legume plants form a nitrogen-fixing symbiosis with Rhizobium bacteria. The bacteria induce the formation of a novel organ called the nodule in which rhizobia reside as intracellular symbionts and convert atmospheric nitrogen into ammonia. During this symbiosis, miRNAs are essential for coordinating the various plant processes required for nodule formation and function.

Induction of Antitumor Immunity by Exosomes Isolated from Cryopreserved Cord Blood Monocyte-Derived Dendritic Cells.

(1) Background: Dendritic cell (DC) vaccination has shown outstanding achievements in cancer treatment, although it still has some adverse side effects. Vaccination with DC-derived exosomes has been thought to overcome the side effects of the parental DCs. (2) Method: We performed the experiments to check the ability of cryopreserved umbilical cord blood mononuclear cell-derived DCs (cryo CBMDCs) and their exosomes to prime allogeneic T cell proliferation and allogeneic peripheral blood mononuclear cell (alloPBMCs) cytotoxicity against A549 lung cancer cells.