Characterization of Isolated Aerobic Denitrifying Bacteria and Their Potential Use in the Treatment of Nitrogen-Polluted Aquaculture Water.

Ammonia and nitrite treatments are the critical steps that must be done to ensure the healthy growth of aquatic animals. The nitrification process is often used for nitrogen removal purposes due to its efficiency and environmentally friendly properties. However, the varied growth rate between ammonia and nitrite-oxidizing bacteria can cause nitrite accumulation, leading to the massive mortality of aquatic animals at high concentrations.

Evaluation of an automated insulated isothermal polymerase chain reaction system for rapid and reliable, on-site detection of African swine fever virus.

Objective: To evaluate the utility of an automated insulated isothermal PCR (iiPCR) system for rapid and reliable on-site detection of African swine fever virus (ASFV) in swine biological samples.

Sample: Lymph node, tissue homogenate, whole blood, serum, spleen, and tonsil samples collected from swine in North and South Vietnam.

Procedures: Analytic sensitivity of the iiPCR system was determined by serial dilution and analysis of 2 samples (swine tissue homogenate and blood) predetermined to be positive for ASFV.