Publications by authors named "Ngwane M"
Article Synopsis
- Development of a non-sputum test for tuberculosis (TB) using lipoarabinomannan (LAM) biomarker concentrations in urine and blood samples was studied to improve diagnostic accuracy, particularly for individuals with and without HIV.
- A diagnostic study in South Africa evaluated LAM levels in urine, plasma, and serum among adults with TB symptoms, finding urine LAM sensitivity of 62% and specificity of 99% using the S4-20 assay; higher sensitivity was observed in HIV-negative participants.
- The findings suggest that while non-sputum specimens can detect LAM for TB diagnosis, improving analyte concentration or signal amplification may be necessary to meet
Testing for mycobacterial lipoarabinomannan (LAM) in urine is a practical but insensitive alternative to sputum testing to diagnose tuberculosis (TB) in people with HIV (PWH). Here, we evaluated urine LAM testing alongside PCR-based tests for Mycobacterium tuberculosis (MTB) DNA in tongue swabs. We hypothesized that the two nonsputum samples would deliver complementary, not redundant, results.
View Article and Find Full Text PDFTransrenal urine cell-free DNA (cfDNA) is a promising tuberculosis (TB) biomarker, but is challenging to detect because of the short length (<100 bp) and low concentration of TB-specific fragments. We aimed to improve the diagnostic sensitivity of TB urine cfDNA by increasing recovery of short fragments during sample preparation. We developed a highly sensitive sequence-specific purification method that uses hybridization probes immobilized on magnetic beads to capture short TB cfDNA (50 bp) with 91.
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