Publications by authors named "Nguyen Q D Tien"
Article Synopsis
- The study aimed to purify a 42 kDa chitinase from Trichoderma asperellum SH16, produced in Nicotiana benthamiana, using a PEG/salt aqueous two-phase system.
- The optimal partitioning conditions identified were 300 g/L PEG 6000 combined with potassium phosphate (PP) showing better efficiency than sodium phosphate (SP), resulting in higher enzymatic activity after purification.
- The purified chitinase demonstrated significant antifungal activity against Sclerotium rolfsii, indicating its potential for use in agriculture and preserving fruits and vegetables postharvest.
Bioproduction of eriodictyol by Escherichia coli engineered co-culture.
World J Microbiol Biotechnol
May 2022
Eriodictyol (ED) is a flavonoid in the flavanones subclass. It is abundantly present in a wide range of medicinal plants, citrus fruits, and vegetables. In addition, ED owns numerous importantly medicinal bioactivities such as inhibition of proliferation, metastasis and induction of apoptosis in glioma cells or inhibition of glioblastoma migration, and invasion.
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- Vietnamese ginseng, also known as Ngoc Linh ginseng, is a valuable herb in Vietnam with various health benefits, including immune system enhancement and anti-cancer properties.
- The study successfully assembled the whole genome of Vietnamese ginseng, revealing it to be approximately 3 billion base pairs long and containing over 79,000 gene models, with many genes linked to triterpenoid biosynthesis identified.
- Researchers cloned and characterized three genes related to squalene monooxygenase and analyzed gene expression levels involved in terpenoid biosynthesis in different plant parts, contributing important data for future studies on ginsenoside production.
Chitinases are enzymes that catalyze the degradation of chitin, a major component of the cell walls of pathogenic fungi and cuticles of insects, gaining increasing attention for the control of fungal pathogens and insect pests. Production of recombinant chitinase in a suitable host can result in a more pure product with less processing time and a significantly larger yield than that produced by native microorganisms. The present study aimed to express the synthetic chi42 gene (syncodChi42), which was optimized from the chi42 gene of Trichoderma asperellum SH16, in Escherichia coli to produce 42 kDa chitinase (Ta-CHI42); then determined the activity of this enzyme, characterizations and in vitro antifungal activity as well as its immunogenicity in mice.
View Article and Find Full Text PDFBackground: Fibrinolytic enzymes, such as Nattokinases from Bacillus species are known to degrade the fibrin blood clots. They belong to serine protease group having commercial applications, such as therapeutic agents and functional food formulation.
Objective: The present study reports some characteristics and fibrinolytic activity of serine protease from B.