Genome Characteristics of the Endophytic Fungus sp. DC2 Isolated from (L.) G. Don.

sp. DC2 is an endophytic fungus that was isolated from the stem of (L.) G.

Draft genome sequencing of halotolerant bacterium sp. DM10 unravels plant growth-promoting potentials.

In this study, strain DM10 was isolated from mangrove roots and characterized as a halotolerant plant growth-promoting bacterium. Strain DM10 exhibited the ability to solubilize phosphate, produce siderophore, show 1-aminocyclopropane-1-carboxylic acid deaminase activity, and hydrolyze starch. The rice plants subjected to a treatment of NaCl (200 mM) and inoculated with strain DM10 showed an improvement in the shoot length, root length, and dried weight, when compared to those exposed solely to saline treatment.

Comparative analysis of microRNA expression profiles in shoot and root tissues of contrasting rice cultivars (Oryza sativa L.) with different salt stress tolerance.

Rice is the second-most important primary crop in the world and one of the most susceptible crops to salt stress. Soil salinization hinders seedling growth and decreases crop yield by inducing ionic and osmotic imbalances, photosynthesis disturbances, cell wall alterations, and gene expression inhibition. Plants have developed a range of defense mechanisms to adapt to salt stress.

Four novel mutations in the androgen receptor gene from Vietnamese patients with androgen insensitivity syndrome.

Background: Androgens and androgen receptor (AR) are critical regulators of the masculinization process in male sexual development. The absence of a functioning AR results in the development of the androgen insensitivity syndrome (AIS), a rare disorder of sexual development (DSD) characterized by the external genitalia feminization, gynecomastia, and impaired spermatogenesis.

Objective: To determine the AR gene mutations associated with male DSD in four unrelated Vietnamese patients.

Molecular Manipulation of the miR396 and miR399 Expression Modules Alters the Response of to Phosphate Stress.

In plant cells, the molecular and metabolic processes of nucleic acid synthesis, phospholipid production, coenzyme activation and the generation of the vast amount of chemical energy required to drive these processes relies on an adequate supply of the essential macronutrient, phosphorous (P). The requirement of an appropriate level of P in plant cells is evidenced by the intricately linked molecular mechanisms of P sensing, signaling and transport. One such mechanism is the posttranscriptional regulation of the P response pathway by the highly conserved plant microRNA (miRNA), miR399.

MicroRNA-Mediated Responses to Cadmium Stress in .

In recent decades, the presence of cadmium (Cd) in the environment has increased significantly due to anthropogenic activities. Cd is taken up from the soil by plant roots for its subsequent translocation to shoots. However, Cd is a non-essential heavy metal and is therefore toxic to plants when it over-accumulates.

Molecular Manipulation of MicroRNA397 Abundance Influences the Development and Salt Stress Response of .

() has been used extensively as a heterologous system for molecular manipulation to genetically characterize both dicotyledonous and monocotyledonous plant species. Here, we report on transformant lines molecularly manipulated to over-accumulate the small regulatory RNA microRNA397 (miR397) from the emerging C monocotyledonous grass model species (). The generated transformant lines, termed plants, displayed a range of developmental phenotypes that ranged from a mild, wild-type-like phenotype, to a severe, full dwarfism phenotype.

Robust and Reproducible -Mediated Transformation System of the C Genetic Model Species .

(green foxtail) has been identified as a potential experimental model system to genetically and molecularly characterise the C monocotyledonous grasses due to its small physical size, short generation time and prolific seed production, together with a sequenced and annotated genome. is the wild ancestor of the cropping species, foxtail millet (), with both species sharing a close evolutionary relationship with the agronomically important species, maize, sorghum, and sugarcane, as well as the bioenergy feedstocks, switchgrass, and . However, an efficient and reproducible transformation protocol is required to further advance the use of to study the molecular genetics of C monocotyledonous grasses.

Reference gene identification for reliable normalisation of quantitative RT-PCR data in .

Background: Quantitative real-time polymerase chain reaction (RT-qPCR) is the key platform for the quantitative analysis of gene expression in a wide range of experimental systems and conditions. However, the accuracy and reproducibility of gene expression quantification via RT-qPCR is entirely dependent on the identification of reliable reference genes for data normalisation. Green foxtail () has recently been proposed as a potential experimental model for the study of C photosynthesis and is closely related to many economically important crop species of the Panicoideae subfamily of grasses, including (maize), (sorghum) and (sugarcane).