Diversity-oriented synthesis of second generation guanidinium-rich transporters toward cell-selective penetration.

Cell-penetrating peptides (CPPs) hold significant promise for intracellular delivery of various cargo molecules such as therapeutics. However, the lack of selectivity remains a critical challenge and limits the clinical application of CPPs. Using an automated peptide synthesizer, we generated a diversity-oriented library of 256 peptidomimetics containing four modified peptoid guanidine-bearing monomers incorporated alternatively with four α-amino acids.

Material-independent film formation and autonomous degradation of Cu-tetrahydroxy-1,4-benzoquinone metal-organic complexes.

Metal-organic complexes (MOCs) have extensively been studied as prominent components in interface engineering. Once the designated missions of MOC films are achieved, or while they are still operational, it is preferred that the films undergo degradation on demand in certain circumstances. Current research on MOC-film degradation predominantly relies on chemical treatment, which can alter the states and conditions of specific systems.

Biphasic water-oil systems for functional augmentation of probiotic nanoencapsulated in luteolin-Fe shells.

Single-cell nanoencapsulation (SCNE) has great potential in the enhancement of therapeutic effects of probiotic microbes. However, the material scope has been limited to water-soluble compounds to avoid non-biocompatible organic solvents that are harmful to living cells. In this work, the SCNE of probiotic with water-insoluble luteolin and Fe ions is achieved by the vortex-assisted, biphasic water-oil system.

Insights on Structure-Passive Permeability Relationship in Pyrrole and Furan-Containing Macrocycles.

Macrocycles have recognized therapeutic potential, but their limited cellular permeability can hinder their development as oral drugs. To better understand the structure-permeability relationship of heterocycle-containing, semipeptidic macrocycles, a library was synthesized. These compounds were created by developing two novel reactions described herein: the reduction of activated oximes by LiBH and the aqueous reductive mono--alkylation of aldehydes using catalytic SmI and stoichiometric Zn.

A Micrometric Transformer: Compositional Nanoshell Transformation of Fe -Trimesic-Acid Complex with Concomitant Payload Release in Cell-in-Catalytic-Shell Nanobiohybrids.

Nanoencapsulation of living cells within artificial shells is a powerful approach for augmenting the inherent capacity of cells and enabling the acquisition of extrinsic functions. However, the current state of the field requires the development of nanoshells that can dynamically sense and adapt to environmental changes by undergoing transformations in form and composition. This paper reports the compositional transformation of an enzyme-embedded nanoshell of Fe -trimesic acid complex to an iron phosphate shell in phosphate-containing media.

Vortex-assisted, nanoarchitectonic manipulation of microparticles with flavonoid-Fe complex in biphasic water-oil systems.

Coordination-driven self-assembly of metal-ligand complexes is a powerful nanoarchitectonic tool for particle engineering, but its usability is limited when using two immiscible coating components. This paper reports that simple vortexing of a biphasic system of Fe ions in water and flavonoids in oil forms nanoshells on individual particles, thereby enabling the utilization of water-insoluble ligands as coating materials. Mechanistic studies suggest that the biphasic mass-transfer equilibrium of flavonoid-Fe species controls the shell formation, with the oil phase acting as a reservoir of coating precursors for continuous coating.

Characterization of PGua, a Guanidinium-Rich Peptoid that Delivers IgGs to the Cytosol via Macropinocytosis.

To investigate the structure-cellular penetration relationship of guanidinium-rich transporters (GRTs), we previously designed PGua, a five-amino acid peptoid containing a conformationally restricted pattern of eight guanidines, which showed high cell-penetrating abilities and low cell toxicity. Herein, we characterized the cellular uptake selectivity, internalization pathway, and intracellular distribution of PGua, as well as its capacity to deliver cargo. PGua exhibits higher penetration efficiency in HeLa cells than in six other cell lines (A549, Caco-2, fibroblast, HEK293, Mia-PaCa2, and MCF7) and is mainly internalized by clathrin-mediated endocytosis and macropinocytosis.

Primary cesarean section rate among full-term pregnant women with non-previous uterine scar in a hospital of Vietnam.

Aim: This article aims to determine the contributing indications for primary cesarean sections among full-term pregnant women with non-previous uterine scars and suggests several potential solutions to reduce the cesarean section rate.

Methods: This is a descriptive study with data being retrospectively collected from electronic medical records (EMRs) at Hanoi Obstetrics & Gynecology Hospital, Vietnam, in 2020. We studied 23,631 women at ≥37 weeks of gestation with non-previous uterine scars.

Enzyme-Mediated Kinetic Control of Fe-Tannic Acid Complexation for Interface Engineering.

Supramolecular self-assembly of Fe and tannic acid (TA) has received great attention in the fields of materials science and interface engineering because of its exceptional surface coating properties. Although advances in coating strategies often suggest that kinetics in the generation of interface-active Fe-TA species is deeply involved in the film formation, there is no acceptable elucidation for the coating process. In this work, we developed the enzyme-mediated kinetic control of Fe oxidation to Fe in a Fe-TA complex in the iron-gall-ink-revisited coating method.

Purging of inbreeding depression within a population of Oxalis alpina (Oxalidaceae).

Premise Of The Study: Variation among individuals in levels of inbreeding depression associated with selfing levels could influence mating system evolution by purging deleterious alleles, but empirical evidence for this association is limited.

Methods: We investigated the association of family-level inbreeding depression and presumed inbreeding history in a tristylous population of Oxalis alpina (Oxalidaceae).

Key Results: Mid-styled individuals possessed the greatest degree of self-compatibility (SC) and produced more autogamous capsules than short- or long-styled individuals.