Shotgun metagenomics sequencing data of root microbial community of Huanglongbing-infected .

Huanglongbing (HLB) is the most serious citrus disease in Vietnam. For the first time, this paper reported root microbial data of HLB-infected grown in Dak Lak Province, Vietnam, for further work toward controlling this disease. Roots of HLB-infected were collected, and the genomic DNA was isolated.

Preparation and Characterization of Chitosan/Starch Nanocomposites Loaded with Ampicillin to Enhance Antibacterial Activity against .

Chitosan/starch nanocomposites loaded with ampicillin were prepared using the spray-drying method by mixing various ratios of chitosan and starch. The morphology of chitosan/starch nanoparticles was studied using a scanning electron microscope (SEM), and the zeta potential value and size distribution were determined by a Nanoparticle Analyzer. The results show that the chitosan/starch nanocomposites have a spherical shape, smooth surface, and stable structure.

Enhancing the antibacterial activity of ampicillin loaded into chitosan/starch nanocomposites against AMR Staphylococcusaureus.

Ampicillin (Amp), an antibiotic, is widely used to treat bacterial infections in humans and livestock, but recently the rate of resistance has increased rapidly. The aim of this work was to enhancing the antibacterial effect of this compound against AMR Staphylococcus aureus via loading Amp into chitosan/starch nanocomposites by spray drying technique. The results showed that the different ratio of chitosan gel and starch gel used in preparing the nanocomposites can affect its properties and performance.

Shotgun metagenomic dataset of root endophytic microbiome of citrus ( L.).

Citrus ( L.) is one of the main fruit crops in Dak Lak Province of Vietnam; however, a dataset on the endophytic microbiome of this plant has yet to be discovered. This article presented the endophytic microbial dataset from roots of healthy L.

Production, Purification, and Characterization of a Cellulase from .

Cellulases are one of the most essential natural factors for cellulose degradation and, thus, have attracted significant interest for various applications. In this study, a cellulase from TKU051 was produced, purified, and characterized. The ideal fermentation conditions for cellulase productivity were 2% carboxymethyl cellulose (CMC) as the growth substrate, pH = 8, temperature of 31 °C, and 4 days of culturing.

Isolation, Plant Growth-Promoting Properties, and Whole-Genome Sequence of a Novel Paenibacillus Species.

This work aimed to isolate and characterize a novel chitin-degrading bacterium from Yok Don National Park, Vietnam, for crop production studies. Among the chitinolytic isolates, strain YSY-4.3 was selected, which grew rapidly and produced a large halo around the colony.

Data on gene cloning, expression, purification, and characterization of the glycoside hydrolase family 11 from .

RB.IBE29 is a chitinolytic bacterium originally isolated from the rhizospheric soil of black pepper grown in Vietnam. This bacterium is a strong biocontrol agent against plant pathogens and possesses a novel chitinase system.

Complete genome sequence data of chitin-degrading RB.IBE29.

This work reports the complete genome sequence of chitinolytic RB.IBE29 recently isolated from the rhizosphere of black pepper cultivated in the Central Highlands region of Vietnam. This bacterium had strong antagonistic activity against phytopathogens and possessed a novel chitinase system.

Assessment of the Chemical Profile and Potential Medical Effects of a Flavonoid-Rich Extract of L. Collected in the Central Highlands of Vietnam.

L. (EPL), a medicinal plant, is widely utilized in the central highlands of Vietnam. This study aims to assess the chemical profile and potential medical effects of an EPL extract rich in flavonoids.

Chitosanase Production from the Liquid Fermentation of Squid Pens Waste by .

Chitosanases play a significant part in the hydrolysis of chitosan to form chitooligosaccharides (COS) that possess diverse biological activities. This study aimed to enhance the productivity of TKU051 chitosanase by fermentation from chitinous fishery wastes. The ideal parameters for achieving maximum chitosanase activity were determined: a squid pens powder amount of 5.

Novel resources of chitinolytic bacteria isolated from Yok Don National Park, Vietnam.

Aims: This study focused on the isolation and characterization of chitinolytic bacteria from Yok Don National Park, Vietnam for future studies regarding biofertilizers and biocontrol agents.

Methods And Results: Chitinolytic bacteria were isolated from soils and chitin flakes soaked in river water at the National Park. On the basis of the halo zones caused by colloidal chitin degradation and colony morphologies, 12 chitinolytic strains were chosen from 15 700 isolates for various examinations.

Novel Anti-Acetylcholinesterase Effect of Champ. Extracts via Experimental and In Silico Studies.

Alzheimer's disease (AD) is the most common form of dementia, which is recorded as a global health issue. Natural acetylcholinesterase inhibitors (AChEIs) are considered a helpful therapy for the management of symptoms of patients with mild-to-moderate AD. This work aimed to investigate and characterize Champ.

Screening and Elucidation of Chemical Structures of Novel Mammalian α-Glucosidase Inhibitors Targeting Anti-Diabetes Drug from Herbals Used by E De Ethnic Tribe in Vietnam.

Among ten extracts of indigenous medicinal plants, the MeOH extract of Stapf. (TTS) showed the most efficient mammalian α-glucosidase inhibition for the first time. The data of screening bioactive parts used indicated that the TTS trunk bark and leaves extracts demonstrated comparable and higher effects compared to acarbose, a commercial anti-diabetic drug, with half-maximal inhibitory concentration (IC50) values of 181, 331, and 309 µg/mL, respectively.

Insights into antiradical mechanism and pro-oxidant enzyme inhibitor activity of walterolactone A/B 6--gallate-β-d-pyranoglucoside originating from Champ. using study.

The ability of a new compound, Wal, (walterolactone A/B 6--gallate-β-d-pyranoglucoside) originating from Champ. as a hydroperoxyl radical scavenger and pro-oxidant enzyme inhibitor was studied . Different mechanisms, reaction locations, and chemical species of Wal in aqueous solution were taken into consideration.

Metagenomic next-generation sequencing of the microbiome dataset from the surface water sample collected from Serepok River in Yok Don National Park, Vietnam.

The Central Highlands region is considered as the center with the highest biodiversity in Vietnam because it has the majority of national parks such as Yok Don, Chu Yang Sin, Bidoup-Nui Ba, Ta Dung, Chu Mon Ray, and Kon Ka Kinh and nature reserves such as Ngoc Linh, Kon Chu Rang, Ea So, Nam Ka, and Nam Nung with different ecosystems [1]. Of the national parks and nature reserves, Yok Don has the most different ecosystem. Yok Don is the second biggest national park, and it is the only national park that conserves dry deciduous dipterocarp forests in Vietnam [2].

Potential of the Liquid Fermentation of Fishery Waste by for Metalloprotease Production.

This study attempted to use fishery processing wastes to produce protease by TKU051. Of the tested wastes, tuna head powder (THP) was found to be the most effective carbon and nitrogen (C/N) source, and the optimal conditions were as follows: 0.811% THP, 0.

Novel α-Amylase Inhibitor Hemi-Pyocyanin Produced by Microbial Conversion of Chitinous Discards.

α-Amylase inhibitors (aAIs) have been applied for the efficient management of type 2 diabetes. The aim of this study was to search for potential aAIs produced by microbial fermentation. Among various bacterial strains, TUN03 was found to be a potential aAI-producing strain, and shrimp heads powder (SHP) was screened as the most suitable C/N source for fermentation.

Expression, purification, and basic properties of a novel domain structure possessing chitinase from Escherichia coli carrying the family 18 chitinase gene of Bacillus velezensis strain RB.IBE29.

Background: Bacillus velezensis possesses numerous chitinolytic enzymes; however, not much is known about the role of its chitinase molecules.

Methods And Results: In this study, the chiA gene, which encodes a novel domain structure possessing family 18 chitinase from B. velezensis RB.

Analysis of endophytic microbiome dataset from roots of black pepper ( L.) cultivated in the Central Highlands region, Vietnam using 16S rRNA gene metagenomic next-generation sequencing.

Vietnam is the most prominent black pepper producer and exporter in the world. In 2020, the cultivated area of black pepper in Vietnam was 132.000 hectares and its production was 270.

Molecular analysis of genes involved in chitin degradation from the chitinolytic bacterium Bacillus velezensis.

Bacillus velezensis RB.IBE29 is a potent biocontrol agent with high chitinase activity isolated from the rhizosphere of black pepper cultivated in the Central Highlands, Vietnam. Genome sequences revealed that this species possesses some GH18 chitinases and AA10 protein(s); however, these enzymes have not been experimentally characterized.

Utilization of Cassava Wastewater for Low-Cost Production of Prodigiosin via TNU01 Fermentation and Its Novel Potent α-Glucosidase Inhibitory Effect.

The purpose of this study was to reuse cassava wastewater (CW) for scaled-up production, via the fermentation of prodigiosin (PG), and to conduct an evaluation of its bioactivities. PG was produced at the yield of high 6150 mg/L in a 14 L-bioreactor system, when the designed novel medium (7 L), containing CW and supplemented with 0.25% casein, 0.

Proteases Production and Chitin Preparation from the Liquid Fermentation of Chitinous Fishery By-Products by .

Chitinous fishery by-products have great application in the production of various bioactive compounds. In this study, TKU051, a protease-producing bacterial strain, was isolated using a medium containing 1% squid pens powder (SPP) as the sole carbon/nitrogen (C/N) source. TKU051 was found to produce at least four proteases with molecular weights of 100 kDa, 57 kDa, 43 kDa, and 34 kDa (determined by the gelatin zymography method).

Production of Sucrolytic Enzyme by by the Bioconversion of Pomelo Albedo as a Carbon Source.

Recently, there has been increasing use of agro-byproducts in microbial fermentation to produce a variety of value-added products. In this study, among various kinds of agro-byproducts, pomelo albedo powder (PAP) was found to be the most effective carbon source for the production of sucrose hydrolyzing enzyme by TKU004. The optimal medium for sucrolytic enzyme production contained 2% PAP, 0.

Bioprocessing of Marine Chitinous Wastes for the Production of Bioactive Prodigiosin.

Recently, microbial prodigiosin (PG) has received much attention due to its numerous beneficial applications. The aim of this study was to establish the bioprocessing of marine chitinous wastes (MCWs) for the cost-effective preparation of PG. Of the MCWs, demineralized shrimp shell powders (de-SSP) were found to be a potential source of carbon/nitrogen (C/N) for PG production by bacterial fermentation using strains.