Obstructive sleep apnea improves with non-invasive hypoglossal nerve stimulation using temporal interference.

Background: Peripheral nerve stimulation is used in both clinical and fundamental research for therapy and exploration. At present, non-invasive peripheral nerve stimulation still lacks the penetration depth to reach deep nerve targets and the stimulation focality to offer selectivity. It is therefore rarely employed as the primary selected nerve stimulation method.

Focal non-invasive deep-brain stimulation with temporal interference for the suppression of epileptic biomarkers.

Introduction: Neurostimulation applied from deep brain stimulation (DBS) electrodes is an effective therapeutic intervention in patients suffering from intractable drug-resistant epilepsy when resective surgery is contraindicated or failed. Inhibitory DBS to suppress seizures and associated epileptogenic biomarkers could be performed with high-frequency stimulation (HFS), typically between 100 and 165 Hz, to various deep-seated targets, such as the Mesio-temporal lobe (MTL), which leads to changes in brain rhythms, specifically in the hippocampus. The most prominent alterations concern high-frequency oscillations (HFOs), namely an increase in ripples, a reduction in pathological Fast Ripples (FRs), and a decrease in pathological interictal epileptiform discharges (IEDs).

Noninvasive Stimulation of Peripheral Nerves using Temporally-Interfering Electrical Fields.

Electrical stimulation of peripheral nerves is a cornerstone of bioelectronic medicine. Effective ways to accomplish peripheral nerve stimulation (PNS) noninvasively without surgically implanted devices are enabling for fundamental research and clinical translation. Here, it is demonstrated how relatively high-frequency sine-wave carriers (3 kHz) emitted by two pairs of cutaneous electrodes can temporally interfere at deep peripheral nerve targets.

Limosilactobacillus caccae sp. nov., a new bacterial species isolated from the human gut microbiota.

Strain Marseille-P3519T isolated from the fecal flora of a 25-year-old healthy French woman was a Gram-positive anaerobic bacterium, non-motile and non-spore forming. The 16S rRNA gene sequence of Marseille-P3519 showed 97.73% of sequence similarity with Limosilactobacillus reuteri DSM 20016, the closest species, phylogenetically.

Multidrug-Resistant Klebsiella pneumoniae Clones from Wild Chimpanzees and Termites in Senegal.

Antibiotic resistance genes exist naturally in various environments far from human usage. Here, we investigated multidrug-resistant Klebsiella pneumoniae, a common pathogen of chimpanzees and humans. We screened antibiotic-resistant K.

Anaerococcus urinimassiliensis sp. nov., a new bacterium isolated from human urine.

To date there are thirteen species validly assigned to the genus Anaerococcus. Most of the species in this genus are anaerobic and of human origin. Anaerococcus urinimassiliensis sp.

Description of sp. nov., a new halophilic bacterium isolated from Senegalian human stool.

Using the taxonogenomics method, we describe strain Marseille-P3801, a new species previously isolated from a salty stool of a 20-year-old man from N'Diop, Senegal. It is a Gram-positive, aerobic and motile bacillus. The major fatty acids are C (59%), C (16%) and C (11%).

Detailed description of strain SIT17 a bacterium isolated from the human gut.

Strain SIT17 was isolated from the stool of a healthy 13-month-old Senegalese boy. It is a Gram-positive, anaerobic, rod-shaped, non-spore-forming and mobile bacterium. It exhibited 92.

Optimization and standardization of the culturomics technique for human microbiome exploration.

Culturomics is a high-throughput culture approach that has dramatically contributed to the recent renewal of culture. While metagenomics enabled substantial advances in exploring the microbiota, culturomics significantly expanded our knowledge regarding the bacterial gut repertoire through the discovery and the description of hundreds of new taxa. While this approach relies on the variation of culture conditions and media, we have tested so far more than 300 conditions since the beginning of culturomics studies.

gen. nov., sp. nov., a new bacterium isolated from the human microbiota.

timonensis gen. nov., sp.

Alcohol pretreatment of stools effect on culturomics.

Recent studies have used ethanol stool disinfection as a mean of promoting valuable species' cultivation in bacteriotherapy trials for Clostridium difficile infections (CDI) treatment with a particular focus on sporulating bacteria. Moreover, the culturomic approach has considerably enriched the repertoire of cultivable organisms in the human gut in recent years. This study aimed to apply this culturomic approach on fecal donor samples treated with ethanol disinfection to evidence potential beneficial microbes that could be used in bacteriotherapy trials for the treatment of CDI.

sp. nov., a new bacterium isolated from human gut microbiota.

sp. nov. strain Marseille-P3557 is a new species isolated from a stool of a Nigerian child with marasmus.

sp. nov., a new bacterium isolated from the stool of a healthy adult.

strain Marseille-P3763 (= CSURP3763) is a new species isolated from the stool of a heathy adult.

Taxono-genomics and description of sp. nov., a new bacterium isolated from stool of healthy patient.

Using the taxono-genomics concept, we describe here a strictly anaerobic Gram-positive bacillus. This strain was isolated from the stool sample of a 50-year-old healthy Bedouin woman. The 16S rRNA gene sequence analysis and the whole-genome sequencing showed that this isolate belonged to the genus in the family .

sp. nov., a new bacteria species isolated from the human gut microbiota.

sp. nov., strain Marseille-P2300 (= CSUR P2300; =DSM102072), is a new bacterial species from the phylum in the family This bacteria species was isolated from the human gut microbiota.

sp. nov., a new bacterium isolated from the human microbiota.

sp. nov., strain Marseille-P3755 (= CSUR P3755 = DSM 103513) is a new bacterial species from the phylum and the family which was isolated from the human gut microbiota.

Extensive culturomics of 8 healthy samples enhances metagenomics efficiency.

Molecular approaches have long led to the assumption that the human gut microbiota is dominated by uncultivable bacteria. The recent advent of large-scale culturing methods, and in particular that of culturomics have demonstrated that these prokaryotes can in fact be cultured. This is increasing in a dramatic manner the repertoire of commensal microbes inhabiting the human gut.

Metagenomic and culturomic analysis of gut microbiota dysbiosis during Clostridium difficile infection.

Recently, cocktail of bacteria were proposed in order to treat Clostridium difficile infection (CDI), but these bacteriotherapies were selected more by chance than experimentation. We propose to comprehensively explore the gut microbiota of patients with CDI compared to healthy donors in order to propose a consortium of bacteria for treating C. difficile.

gen. nov., sp. nov., a new bacterium isolated from the human gut microbiota.

gen. nov., sp.

Genome sequence and description of sp. nov., a new anaerobic bacterium isolated from the human gut.

sp. nov., strain Marseille-P2653 (= CSUR; P2653=DSM103120) is a new bacterial species belonging to the phylum in the family that was isolated from the human gut microbiota.

sp. nov., sp. nov. and sp. nov., three new bacterial species identified from human gut microbiota.

We report here the main characteristics of strain Marseille-P3740 (CSUR P3740), strain Marseille-P3763 (CSUR P3763) and strain Marseille-P3660 (CSUR P3660), which were isolated using culturomics from the human gut microbiota of healthy individuals living in Marseille.

Noncontiguous finished genome sequence and description of gen. nov., sp. nov., a new member of the family isolated from human colon.

Strain Marseille-P2645 was isolated in a colon sample from a Frenchwoman who underwent a colonoscopy. Bacterial cells were Gram negative, non-spore forming, mobile and strictly anaerobic. The genome of strain Marseille-P2645 is 3 950 441 bp long and contains 3374 protein-coding genes.

[Post-hysterectomy vesicovaginal fistula].

Objective: Describe the epidemiology, diagnosis and treatment of vesicovaginal fistula (VVF).

Patients And Methods: We conducted a retrospective descriptive study of all cases of VVF secondary to hysterectomy. The following parameters were studied: age, parity, indication for hysterectomy, risk factors, the consultation period, the anatomical type of VVF, the paraclinical, the surgical approach and results of the cure.

Gonadectomy induces site-specific differences in nociception in rats.

High prevalence of chronic orofacial pain in women and its relationship with ovarian states suggest that ovarian hormones may be involved in the control of orofacial nociception. Since the interaction between ovarian hormones and nociception seems more evident in the orofacial area than in many other parts of the body, a possible site specificity of an ovarian hormone effect on nociception was tested in rats. Two nociceptive tests were applied to three groups of male rats (n=46) and three groups of female rats (n=46), that were gonadectomised (n=17), sham-operated (n=15) or intact (n=14).