Fabrication of lateral flow immunoassay strip for rapid detection of acute hepatopancreatic necrosis disease.

Acute hepatopancreatic necrosis disease (AHPND) is a contagious disease for the shrimp cultivation, thus early detection of disease is an unmet need. This present study documented for the first time a simple lateral flow immunoassay (LFIA) strip using polyclonal antibodies was created for the rapid detection both of PirA and PirB protein simultaneously. LFIA method based on the principle of sandwich format.

Production of polyclonal antibody against the recombinant PirB protein of Vibrio parahaemolyticus.

Background: Acute hepatopancreatic necrosis disease (AHPND) is caused by toxin-producing strains of Vibrio parahaemolyticus which contain deadly binary toxins PirA and PirB encoded in pVA1 plasmid. The polyclonal antibodies against PirB protein could be used to develop immunochromatographic test strip for in-field diagnosis of AHPND.

Results: In this study, PirB gene was amplified, cloned, and expressed in E.

Generation and evaluation of polyclonal antibodies specific for ToxA from causing acute hepatopancreatic necrosis disease (AHPND) in shrimp.

Acute Hepatopancreatic Necrosis Disease (AHPND) is a newly emerging shrimp disease with mortality up to 100 percent caused by which carries a plasmid encoding for two toxins, ToxA and ToxB. In 2013, the Global Aquaculture Alliance (GAA) estimated shrimp farming decline in Asia accounted for 1-billion US dollar lost. Currently, diagnosis using PCR method does not meet the demand of detection, which is based on antigen-antibody interaction, has not been developed yet.