Novel highly-multiplexed AmpliSeq targeted assay for genetic surveillance use cases at multiple geographical scales.

Although the power of genetic surveillance tools has been acknowledged widely, there is an urgent need in malaria endemic countries for feasible and cost-effective tools to implement in national malaria control programs (NMCPs) that can generate evidence to guide malaria control and elimination strategies, especially in the case of . Several genetic surveillance applications ('use cases') have been identified to align research, technology development, and public health efforts, requiring different types of molecular markers. Here we present a new highly-multiplexed deep sequencing assay (Pv AmpliSeq).

Colorimetric Detection of Carcinogenic Alkylating Fumigants on a Nylon 6 Nanofibrous Membrane. Part II: Self-Catalysis of 2-Diethylaminoethyl-Modified Sensor Matrix for Improvement of Sensitivity.

A nylon 6 nanofibrous membrane (N6NFM) was covalently modified with 2-diethylaminoethylchloride (DEAE-Cl) to provide self-catalytic functions to facilitate the formation of color compounds in reactions of 4-( p-nitrobenzyl)pyridine with alkylating fumigants. The 2-diethylaminoethyl group on the DEAE-Cl-modified N6NFM (DEAE@N6NFM) enables effective elimination of hydrohalogenic acids from intermediates that were formed from reactions between the alkylating fumigants and NBP and consequently improve their detection sensitivities, especially for 1,3-dichloropropene at room temperature. Moreover, DEAE@N6NFM can be recycled and reused multiple times without obvious loss in the sensing functions or any noticeable material damage.

Partial mitochondrial DNA sequences suggest the existence of a cryptic species within the Leucosphyrus group of the genus Anopheles (Diptera: Culicidae), forest malaria vectors, in northern Vietnam.

Background: During the last decade, Southeast Asian countries have been very successful in reducing the burden of malaria. However, malaria remains endemic in these countries, especially in remote and forested areas. The Leucosphyrus group of the genus Anopheles harbors the most important malaria vectors in forested areas of Southeast Asia.