Structural and Kinetic Characterization of Hyperthermophilic NADH-Dependent Persulfide Reductase from .

NADH-dependent persulfide reductase (Npsr) has been proposed to facilitate dissimilatory sulfur respiration by reducing persulfide or sulfane sulfur-containing substrates to HS. The presence of this gene in the sulfate and thiosulfate-reducing DSM 4304 and other hyperthermophilic appears anomalous, as is unable to respire S and grow in the presence of elemental sulfur. To assess the role of Npsr in the sulfur metabolism of DSM 4304, the Npsr from was characterized.