Velocity magnitude estimation with linear arrays using Doppler bandwidth.

The dependence of pulsed wave Doppler bandwidth on parameters typical of linear transducer arrays used in commercial Duplex and color flow mapping systems is investigated experimentally. For a single flow line it is observed that this bandwidth generally depends not only on the scatterer velocity and the beam-to-flow angle, but also on the flow line range and orientation. This is due to the fact that in Duplex and color flow systems the transducer is differently focused in the scan and elevation planes and its aperture and focal lengths are often made to vary, depending on the distance of the flow line from the transducer.

Subharmonic backscattering from ultrasound contrast agents.

The ultrasonic contrast of blood in tissue, which is needed for ultrasonic estimation of tissue perfusion, can be increased by injecting the blood with bubbles or hollow microspheres. It has been shown that an even greater improvement in contrast can be obtained by using the subharmonic generated by irradiated microspheres. By obtaining analytical solutions to the modified RPNNP equation for a coated microbubble, the relationship between the physical parameters of the encapsulated bubble and the threshold pressure is established.

Subharmonic generation from ultrasonic contrast agents.

Ultrasonic contrast agents are used to enhance backscatter from blood and thus aid in delineating blood from surrounding tissue. However, behaviour of contrast agents in an acoustic field is nonlinear and leads to harmonic components in the backscattered signal. Various research groups have investigated second-harmonic emissions.

Onset delay of acoustic second harmonic backscatter from bubbles or microspheres.

A well-known method of enhancing blood detectability in ultrasound imaging of tissues detects the second harmonic of the incident radiation, which is generated by ultrasound contrast agents in the form of bubbles or microspheres that may have been injected into the blood. We report here a delay in the onset of the backscattered second harmonic with respect to the backscattered first harmonic for these agents. This effect, which should limit the axial resolution attainable with harmonic imaging, is investigated by simulation as well as experiment, and its dependence on the incident ultrasonic amplitude and microsphere parameters is established.

Advantages of subharmonic over second harmonic backscatter for contrast-to-tissue echo enhancement.

It is shown experimentally that backscatter from two ultrasonic contrast agents suspended in water or saline contains subharmonics of the incident frequency that are stronger than those backscattered at the same incident pressure from chicken breast. It is also shown that the ratio of subharmonic backscattered from contrast to that backscattered from tissue, is stronger than the ratio of backscattered second harmonic. In consequence, blood that contains contrast should be more easily detectable with respect to tissue if the subharmonic, rather than the second harmonic, is used for imaging.

Estimation of axial blood velocity using the Doppler equation corrected for broadening.

To estimate axial velocity for vessels that are so narrow that their width is comparable to the minimum length of the range cell, it has been customary to substitute the maximum Doppler frequency fmax into the classical Doppler equation [eqn (1)]. It was shown here that this ignores transit time broadening, which can lead to significant errors at large beam-to-flow angles. We use a relation [eqn (2)], which does take this broadening into account, and give in vivo experimental proof of this allows accurate estimation of the axial velocity even when the range cell extends across the whole vessel lumen.

Second harmonic characteristics of the ultrasound contrast agents albunex and FSO69.

Techniques necessary for measurement of the second harmonic of the insonifying frequency backscattered from ultrasonic contrast agents are described, and used to determine this characteristic for the agents Albunex and FSO69. The results confirm theoretical predictions that scattered second harmonic pressure is proportional to the square of the incident pressure. Because contrast agents of the type investigated improve discrimination of blood echoes against tissue echoes by means of the second harmonic of the insonifying frequency, these results allow a comparison of the relative merits of Albunex and FSO69 for harmonic imaging.

Demonstration of three-dimensional vector flow estimation using bandwidth and two transducers on a flow phantom.

A method of performing three-dimensional (3-D) velocity vector estimation with two transducers is demonstrated on a flow phantom using the Doppler spectra's mean frequencies and bandwidths. The results are compared with 3-D vector estimates computed from Doppler mean frequencies obtained with a five-transducer system. It is shown that the two-transducer vector Doppler system which uses bandwidth can improve on the accuracy of a three-transducer vector Doppler system which relies only on Doppler mean frequencies.

Improved blood velocity estimation using the maximum Doppler frequency.

In vessels whose diameter is smaller than the length of the range cell or measurement volume, the maximum blood velocity is often calculated from the maximum frequency of the Doppler spectrum, using the classical Doppler equation. It is shown that the accuracy of this procedure is significantly improved at large beam-to-flow angles, if a correction for transit time broadening is made. This finding is based on the demonstration that the maximum frequency of the Doppler spectrum depends only on the maximum velocity passing through the measurement volume, but in a manner which is a function both of the Doppler shift frequency as well as the transit time broadening associated with the passage of scatterers through the beam width.

On the spectral properties of Doppler thread phantoms.

It is shown that some of the threads used in Doppler phantoms have a repetitive structure which leads to peaks in the angular distribution of the backscattered power at beam axis-to-flow angles of theta = 90 degrees and approximately theta = 70 degrees. This nonuniform scattering does not significantly interfere with modelling the Doppler spectrum peak as a function of velocity and beam-to-thread angle, but makes it impossible to model the spectral width as a function of these parameters. A new plaited structure is described which has a periodicity too small to lead to subsidiary reflection peaks, and which has a more uniform backscattering profile than the other threads studied.

Comparison of conventional and transverse Doppler sonograms.

When measuring flow velocity using the conventional ultrasonic Doppler effect, beam axis-to-flow angles approaching 90 degrees are avoided as the Doppler spectrum frequency shift is known to go to zero at this angle. In this paper, the conventional Doppler technique is compared with the transverse Doppler method, in which the Doppler spectrum bandwidth is used to estimate flow, allowing flow to be probed at 90 degrees. The comparison is made using a moving thread flow phantom capable of executing various velocity profiles.

Invariance of the Doppler bandwidth with range cell size above a critical beam-to-flow angle.

For a sound beam impinging on a blood vessel, with a range cell much smaller than the vessel diameter, it is known that the breadth of the echo Doppler spectrum is proportional to the velocity of the flow through the range cell. As the range cell is lengthened to include a greater range of velocities, the spectrum is expected to widen proportionately. It is shown theoretically, and confirmed experimentally, that if the beam-to-flow angle is greater than a critical value, the Doppler spectrum bandwidth is independent of the length of the range cell, and depends only on the maximum velocity encompassed by it.

Second harmonic ultrasonic blood perfusion measurement.

In vitro and in vivo testing of a recently introduced method of evaluating blood perfusion is presented, where the Doppler shift of the second harmonic component of the backscattered echo is measured. Central to this measurement is the administration of a galactose-based contrast agent (Schering AG, Berlin, Germany, SHU-508 or derivative) which has been shown in vitro to exhibit extraordinary nonlinear backscattering properties. Two types of experiments are described: in vitro studies on excised sheep kidneys and in vivo studies on living rabbits.

Simulated capillary blood flow measurement using a nonlinear ultrasonic contrast agent.

This paper proposes a system for measuring slow, small volume blood flow, such as that found in the capillary beds. The method relies on the injection of a strongly nonlinear echocardiographic contrast agent, whose echoes are then analyzed by a modified Doppler process. The contrast agent is necessary to increase the signal-to-clutter ratio from the small blood volume, and to distinguish the blood movement from other moving structures, such as vessel walls or surrounding tissue.

Experimental proof of Doppler bandwidth invariance.

A line flow of scatterers crossing the sound field produced by a focused circular transducer at uniform velocity originates a quasi-triangular Doppler spectrum. It is known that the spectrum shape and width depend on the line flow to beam axis angle, as well as on the transducer geometry. It has recently been theoretically predicted that this spectrum width is independent of the flow line location in the sound field.

Doppler ultrasound technique for measuring capillary-speed flow velocities with strong stationary echoes.

Ultrasound Doppler is widely used for low measurement in both medicine and industry, having the advantages of being non-invasive and comparatively simple and therefore inexpensive. The technique has not however been used for capillary blood flow measurement, because of the relatively low velocities encountered and because of the presence of strong interfering signals from the encompassing tissue. An ultrasound Doppler system capable of measuring flow velocities of one millimetre per second in the presence of one thousand times stronger interfering signals is described, as well as test results using both thread and flow phantoms.

Invariance of the Doppler bandwidth with flow displacement in the illuminating field.

It is known that if single frequency continuously transmitted ultrasound or electromagnetic energy is reflected from "straight line flow," defined here as one or more scatters moving with constant velocity along an infinite straight line, the Doppler effect will shift the echo spectrum center frequency from the transmitted value, and broaden its bandwidth. It is proved that if such straight line flow is shifted laterally or in range anywhere in the field, i.e.

Bubble sizing with high spatial resolution.

The authors propose a technique that allows them to size bubbles with the same accuracy as with the double-frequency method and to locate them with the same range resolution as with the pulsed Doppler velocimeter. They demonstrate that the signal scattered by the bubble insonified by a high-frequency pulsed ultrasonic field and a low-frequency pumping field is a low-frequency signal sampled at the repetition frequency rate and in which the amplitude is maximum when the bubble resonates. However using a conventional Doppler flowmeter, the maximum amplitude is not detectable when the repetition frequency is a multiple of the pump frequency.

The future of clinical engineering in the 1990's.

The problems in hospitals which led to the development of the clinical engineering profession are described along with recent changes in the hospital environment. The authors discuss how the profession is adapting to these changes. Also discussed is the tendency for BMETs to move into clinical engineering roles.

Split-spectrum processing: analysis of polarity threshold algorithm for improvement of signal-to-noise ratio and detectability in ultrasonic signals.

A polarity thresholding algorithm that has recently been developed for split-spectrum processing for ultrasonic coherent noise reduction is theoretically analyzed to evaluate its performance. The probability density function (PDF) of the output of the algorithm is derived and used to calculate the theoretical signal-to-noise ratio (SNR) enhancement and the receiver operating characteristics. The performance limits of the algorithm are also established.

Rocky Mountain spotted fever in Georgia, 1961-75: analysis of social and environmental factors affecting occurrence.

For the period of 1961 through 1975, 10 geographic and sociologic variables in each of the 159 counties of Georgia were analyzed to determine how they were correlated with the occurrence of Rocky Mountain spotted fever (RMSF). Combinations of variables were transformed into a smaller number of factors using principal-component analysis. Based upon the relative values of these factors, geographic areas of similarity were delineated by cluster analysis.