Polyphasic characterization and genomic insights into Nocardioides turkmenicus sp. nov. isolated from a desert soil.

Strain KC13, a novel desert-adapted, non-motile, Gram-stain-positive, rod-shaped, aerobic bacterium, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan and characterised by a polyphasic approach. Phylogenetic analysis based on 16S rRNA sequences revealed that strain KC13 was a member of the genus Nocardioides, and formed a distinct cluster with Nocardioides luteus DSM 43366 (99.3% sequence identity), Nocardioides albus DSM 43109 (98.

Saccharopolyspora soli sp. nov., isolated from Northern Cyprus soil.

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain K220, was isolated from soil collected from Cape Andreas (Zafer Burnu), Northern Cyprus, and subjected to a polyphasic taxonomic approach. The organism was shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Saccharopolyspora. 16S rRNA gene sequence analysis of strain K220 showed that it is closely related to the type strains of Saccharopolyspora maritima 3SS5-12, Saccharopolyspora kobensis JCM 9109 and Saccharopolyspora hirsuta ATCC 27875 with 97.

sp. nov., isolated from the top soil layer on basaltic material in Turkey.

An actinobacterium, designated 14C53, was isolated from a soil sample on basaltic material from Samsun, Turkey. The growth ranges for NaCl concentration and pH of strain 14C53 were quite limited and the growth temperature range of the strain was 20-37 °C, with an optimum at 28 °C. Phylogenetic analysis of 16S rRNA gene sequences revealed that strain 14C53 was most closely related to A8036 (98.

Saccharopolyspora karakumensis sp. nov., Saccharopolyspora elongata sp. nov., Saccharopolyspora aridisoli sp. nov., Saccharopolyspora terrae sp. nov. and their biotechnological potential revealed by genome analysis.

Exploration of unexplored habitats for novel actinobacteria with high bioactivity potential holds great promise in the search for novel entities. During the course of isolation of actinobacteria from desert soils, four actinobacteria, designated as 5K548, 7K502, 16K309 and 16K404, were isolated from the Karakum Desert and their bioactivity potential as well as taxonomic provenances were revealed by comprehensive genome analyses. Pairwise sequence analyses of the 16S rRNA genes indicated that the four strains are representatives of putatively novel taxa within the prolific actinobacterial genus Saccharopolyspora.

Comprehensive genome analysis of a novel actinobacterium with high potential for biotechnological applications, Nonomuraea aridisoli sp. nov., isolated from desert soil.

During a study to isolate such actinobacteria with unique metabolic potential, a novel actinobacterium, designated KC333, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of the strain was investigated using a polyphasic approach. Phylogenetic analysis of the 16S rRNA gene sequence showed that the strain was most closely related to Nonomuraea terrae CH32 (99.

sp. nov. isolated from internal organs of apparently healthy rainbow trout.

Two yellow-pigmented isolates, F-60 and F-392, were isolated from the internal organs of an apparently healthy rainbow trout (). The strains were identified as members of the genus based on the results of 16S rRNA gene sequence analysis. Strains F-60 and F-392 had the highest 16S rRNA gene sequence identity level of 97.

A Novel 4H-Chromen-4-One Derivative from Marine Streptomyces ovatisporus S4702 as Potential Antibacterial and Anti-Cancer Agent.

Background: Marine actinomycetes are among indispensable sources of natural bioactive compounds with unique antimicrobial and anti-cancer activities.

Objective: Herein, it was aimed to elucidate the bioactive potential of a marine-derived Streptomyces ovatisporus S4702T, isolated previously.

Methods: Streptomyces ovatisporus S4702 was cultured in N-Z Amine broth, and extraction was carried out using different organic solvents.

sp. nov. and sp. nov., two novel with antimicrobial activity isolated from lake sediment.

Two novel , designated strains YC419 and YC504, were isolated from a sediment sample collected from Lake Yeniçağa, Bolu Province, Turkey. Chemotaxonomic and morphological characteristics of isolates were found to be typical of members of the genus . Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain YC419 was most closely related to NBRC 13094 (99.

Flavobacterium turcicum sp. nov. and Flavobacterium kayseriense sp. nov. isolated from farmed rainbow trout in Turkey.

During a study on culturable microorganisms from fish farms, four yellow-pigmented gram negative, rod shaped isolates, F-47, F-339, F-380 and F-400, were recovered from rainbow trout samples exhibiting clinical signs. Based on 16S rRNA gene sequence analysis, the strains were identified as members of the genus Flavobacterium. Strains F-47 and F-380 shared the highest 16S rRNA gene sequence identity level of 97.

sp. nov., isolated from soil.

A novel actinomycete, designated strain HC44, was isolated from a soil sample collected from Hacibektaş, Turkey, and characterized using a polyphasic approach. The strain had morphological characteristics and chemotaxonomic properties identical to those of members of the genus . Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that HC44 clustered with members of the genus and the highest 16S rRNA gene sequence similarity values were obtained with NBRC 13094 (97.

Streptomyces boncukensis sp. nov., isolated from saltern soil.

A novel Gram-stain positive, aerobic, non-motile Actinobacterium, designated strain SB3404, was isolated from saltern soil collected from Boncuk Saltern, Sungurlu-Çorum, Turkey, and subjected to a polyphasic taxonomic approach. The organism has shown to have phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain SB3404 showed that it is closely related to Streptomyces albus NBRC 13014 (97.

sp. nov., isolated from hazelnut orchard soil.

A novel actinobacteria, isolate A7024, was isolated from commercial hazelnut orchard soil sample which was collected at Duzce, West Black Sea region, Turkey. A polyphasic taxonomic study was carried out to determine the status of this isolate. The phylogenetic tree reconstructed using the neighbour-joining algorithm based on 16S rRNA gene sequences indicated that isolate A7024 was positioned within the members of the genus with the highest sequence similarity (97.

Streptomyces boluensis sp. nov., isolated from lake sediment.

A novel Gram-stain positive, aerobic, non-motile actinobacterium, designated strain YC537, was isolated from lake sediment collected from Yenicaga Lake, Bolu, Turkey, and subjected to a polyphasic taxonomic approach. The organism had phylogenetic, chemotaxonomic, cultural and morphological properties consistent with its classification in the genus Streptomyces. 16S rRNA gene sequence analysis of strain YC537 showed that it is closely related to the type strain of Streptomyces ziwulingensis F22 (97.

Shimazuella alba sp. nov. isolated from desert soil and emended description of the genus Shimazuella Park et al. 2007.

A novel, Gram-stain-positive bacterium, designated KC615, was isolated from desert soil which was collected from the Karakum Desert, Turkmenistan. Phylogenetic analysis based on an almost-complete 16S rRNA gene sequence showed that isolate KC615 formed a monophyletic clade with Shimazuella kribbensis KCTC 9933, sharing 98.2% similarity and polyphasic taxonomic studies confirmed the affiliation of the strain to the genus Shimazuella.

Nonomuraea basaltis sp. nov., a siderophore-producing actinobacteria isolated from surface soil of basaltic parent material.

A Gram-stain-positive, aerobic, spore-forming actinobacterial strain, designated 160415, was isolated from a surface soil sample, which was formed on basaltic parent material, collected from Samsun, Turkey. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain 160415 clustered closely with species of the genus Nonomuraea, and showed the highest sequence similarity to Nonomuraea zeae NEAU-ND5, Nonomuraea candida HMC10 and Nonomuraea turkmeniaca DSM 43926 with 99.1%, 98.

Genome-based classification of three novel actinobacteria from the Karakum Desert: sp. nov., sp. nov. and sp. nov.

Three isolates, 5K138, 8K307 and KC603, with typical morphological characteristics of members of the genus were obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliations of the strains. The strains showed chemotaxonomic properties consistent with their classification as representing members of the genus such as ll-diaminopimelic acid in the cell wall peptidoglycan, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol as major polar lipids as well as MK-9(H) as a major menaquinone.

sp. nov., isolated from desert soil and reclassification of as a later heterotypic synonym of .

A novel actinobacterial strain, designated 13K301, was isolated from a soil sample collected from the Karakum Desert, Turkmenistan. The taxonomic position of strain 13K301 was revealed by using a polyphasic approach. On the basis of 16S rRNA gene sequence analysis, strain 13K301 belongs to the genus and had highest sequence similarity to '' S10 (99.

Phylogenomic revision of the family , reclassification of as comb. nov. and a taxonomic home for the genus in the family .

In recent years, the results of genome-based phylogenetic analyses have contributed to microbial systematics by increasing the availability of sequenced microbial genomes. Therefore, phylogenomic analysis within large taxa in the phylum has appeared as a useful tool to clarify the taxonomic positions of ambiguous groups. In this study, we provide a revision of the actinobacterial family using a large collection of genome data and phylogenomics approaches.

Genome-based classification of Micromonospora craterilacus sp. nov., a novel actinobacterium isolated from Nemrut Lake.

A novel actinobacterial strain, designated NA12, was isolated from coastal sediment sample of Nemrut Lake, a crater lake in eastern Anatolia, Turkey. The taxonomic position of the strain was established using a polyphasic approach. Cultural and chemotaxonomic characteristics of the strain were consistent with its classification within the family Micromonosporaceae.

Polyphasic classification of strains isolated from the Karakum Desert and description of sp. nov., sp. nov., sp. nov. and sp. nov.

Five actinobacteria isolates, KC201, KC401, KC310, KC712 and 6K102, were recovered from the Karakum Desert during an investigation of novel actinobacteria with biotechnological potential. A polyphasic approach confirmed the affiliation of the strains to the genus . The strains showed chemotaxonomic and morphological properties consistent with their classification in the genus .

Micromonospora orduensis sp. nov., isolated from deep marine sediment.

A novel actinobacterial strain, designated S2509, was isolated from marine sediment collected by a dredge at a depth of 45 m along Melet River offshore of the southern Black Sea coast, Ordu, Turkey. The cell wall peptidoglycan of strain was found to contain meso-diaminopimelic acid and 3-OH-diaminopimelic acid. The whole cell sugars detected were arabinose, glucose, rhamnose, ribose and xylose.

sp. nov., isolated from the Karakum Desert.

An isolate, 13K206, with typical morphological characteristics of the genus was obtained during a study searching for novel actinobacteria with biosynthetic potential from the Karakum Desert. A polyphasic approach was adopted to determine taxonomic affiliation of the strain. The strain showed chemotaxonomical properties consistent with its classification in the genus such as - and 3-OH-Apm in the cell-wall peptidoglycan, xylose in whole-cell hydrolysate and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.

Kribbella turkmenica sp. nov., isolated from the Karakum Desert.

A novel actinobacterial strain, designated 16K104, was isolated from desert soil collected from the Karakum Desert and characterized using a polyphasic approach to clarify its taxonomic position. Strain 16K104 was found to have chemotaxonomic and morphological properties consistent with classification in the genus Kribbella. The strain shared the highest 16S rRNA gene sequence similarity with Kribbella albertanoniae BC640 (99.

Jiangella anatolica sp. nov. isolated from coastal lake soil.

A novel actinobacterial strain, designated GTF31, was isolated from a coastal soil sample of Gölcük Lake, a crater lake in southwest Anatolia, Turkey. The taxonomic position of the strain was established using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences and showed that the strain is closely related to Jiangella gansuensis DSM 44835 (99.