Publications by authors named "Neville De Young"
In the development and progression of cancer, tumor suppressor genes may be silenced by mechanisms such as methylation. Thus the discovery of new genes silenced by methylation may uncover new tumor suppressor genes, and improve our understanding of cancer biology. In this study we investigated the methylation of 19 genes in esophageal squamous cell carcinoma.
View Article and Find Full Text PDFBackground: Barrett's esophagus (BE) is the metaplastic replacement of squamous with columnar epithelium in the esophagus, as a result of reflux. It is the major risk factor for the development of esophageal adenocarcinoma (EAC). Methylation of CpG dinucleotides of normally unmethylated genes is associated with silencing of their expression, and is common in EAC.
View Article and Find Full Text PDFAim: To measure the frequency of DNA methylation of the tissue inhibitor of metalloproteinase 3 (TIMP3) promoter and relate this to any change of gene expression in esophageal squamous cell carcinoma in patients from a region of high incidence in China.
Methods: Cancer cell lines were treated with or without the demethylating reagent 5-aza-2'-deoxycytidine. Methylation of the TIMP3 promoter was assessed in three regions by melt curve analysis and its expression was assessed by real-time RT-PCR.
Article Synopsis
- Study examined how Metallothionein 3 (MT3) impacts growth in oesophageal cancer cells, focusing on its expression and promoter methylation.
- MT3 was not expressed in one cell line, and methylation was higher in this non-expressing line; treatment reduced methylation and increased expression in all lines.
- In patient samples, MT3 was methylated in 52% of tumors and linked to reduced expression, but this methylation did not correlate with survival or other clinical factors.
Background: Electronic voting systems have been used in various educational settings with little measurement of the educational impact on students. The goal of this study was to measure the effects of the inclusion of an electronic voting system within a small group tutorial.
Method: A prospective randomised controlled trial was run at the Royal Adelaide Hospital, a teaching hospital in Adelaide, Australia.