A Collaborative and Scalable Geospatial Data Set for Arctic Retrogressive Thaw Slumps with Data Standards.

Arctic permafrost is undergoing rapid changes due to climate warming in high latitudes. Retrogressive thaw slumps (RTS) are one of the most abrupt and impactful thermal-denudation events that change Arctic landscapes and accelerate carbon feedbacks. Their spatial distribution remains poorly characterised due to time-intensive conventional mapping methods.

Prospects for the development of the electronic prescription system in the conditions of the modern pharmaceutical market of Russia.

Background: This research aims to assess the level of awareness and readiness for the utilization of Electronic Prescription System (EPS) among medical professionals and patients, as well as to identify their opinions regarding the advantages and disadvantages of this system.

Methods: The study was conducted in Russia among 423 respondents, including medical professionals and patients, with an average age of 40 years.

Results: The awareness of EPS among medical professionals (20%) and patients (45.

[Balloon dilation treatment of primary obstructive megaureter in children].

Purpose: Investigation of the results of ureteral stricture single balloon dilation in children with primary obstructive megaureter.

Materials And Methods: Since 2012 to 2020 79 children (65 boys and 15 girls) with primary obstructive megaureter of II and III grades who had 92 affected ureters were operated on by method of ureteral stricture balloon dilation. Duration of postoperative stenting period was Me=68 [48; 91] days, bladder catheterization period - Me=15 [5; 61] days.

Luminescent polyelectrolytes with antiviral activity.

Radical polymerization was used to synthesize and characterize (co)polymers with sodium styrenesulfonate (NaSS), 4-methacryloylamidosalicylic acid (MASA), and -vinylpyrrolidone, which have a low cytotoxicity and a high antiviral activity against the human respiratory syncytial virus. The interaction of copolymers with Tb ions was studied. The complexes formed in dilute aqueous solutions at a concentration of MASA units ⩽ 1 · 10 mol L demonstrate a strong luminescence.

Evaluation of the Neuroprotective Effect of Magnesium-Bis-(2-Aminoethanesulfonic)-Butanedioate in Simulated Ischemic Stroke in Rats.

Sudden loss of blood flow to an area of the brain causes ischemic stroke, which leads to the loss of nerve function in the brain. The brain tissue leads to the death of brain cells in less than a few minutes due to the lack of oxygen and nutrients. This study aimed to evaluate the effectiveness of pharmacological correction of the consequences of ischemic stroke with a new derivative of taurine magnesium-bis-(2-aminoethanesulfonic)-butanedioate under laboratory code LKHT 3-17 in rats.

Investigation of Taurine Derivative Magnesium-Bis-(2-Aminoethanesulfonic)-Butadioateon Alleviation of Neurological Defects in Simulated Hemorrhagic Stroke in Rats.

Stroke or ischemia is caused by a blockage in a specific blood vessel that partially or completely reduces the blood flow to the brain. Nutritional factors such as antioxidants and healthy eating patterns are important variables in preventing stroke. Molecular composition properties such as molecular binding and screening can be used to evaluate the specific activity and morphological changes.

[SURFACE PLASMON RESONANCE: APPROACHES AND PERSPECTIVES OF APPLICATION FOR VIRUS-SPECIFIC INTERACTIONS INVESTIGATIONS].

In the review the published data on the use of surface plasmon resonance methods for the study of individual viral proteins as well as intact viruses are reviewed. . The principles of the method, its benefits and peculiarities of its use to study viruses are introduced.

[INFLUENCE OF DOXORUBICIN AND ETOPOSIDE ON THE CD 95 MEDIATED APOPTOSIS IN EBV INFECTED LYMPHOMA CELLS BL-41 AND DG-75].

The aim of work was to study the effect of anticancer drugs on the process of CD 95 mediated apoptosis in BL-41 and DG-75 infected with Epstein-Barr virus. Studies of the effect of anticancer drugs "Doxorubicin" "Ebewe" and "Vepesid" (Etoposide) on the apoptosis in EBV infected cells using cytomorphological methods, spectrophotometry and PCR carried out. The influence of the tested drugs in cell culture was assessed by calculating the CC50.

Study of adenovirus reproduction in different lymphoblastoid cell lines.

The comparative characteristic of the reproduction of adenovirus serotypes 2 and 5 (HAdV-C2 and -C5) in the various lymphoblastoid cell lines were studied. Rapid formation of infectious viruses in Raji, MP-1, Namalwa, BJAB, MT4 and Jurkat cells was marked and it was found to be close to the level of viruses during reproduction in permissive Hep-2 epithelial cells. Yield of infective adenovirus was low in B95-8 cells, which were chronically infected with Epstein-Barr virus (EBV).

[Modelling of the adeno-herpetic infection in lymphoblastoid cell cultures].

Viral infections take the key place in medical practice. A large group of diseases are caused by adenoviral and herpes infection. As a rule, the investigations carried out in scientific laboratories are directed to the study of certain aspects of the interaction between the virus and the cell on the model of single infection.

[AntiEBV activity of 6-azacytidine and its derivatives].

Epstein-Barr virus (EBV) causes several lymphoproliferative diseases, lesions of the central and peripheral nervous systems. Spectrum of etiotropic medicines against EBV is limited. The paper presents the anti-virus research of modified nucleoside with a wide spectrum of action - 6-azacytidine and its derivatives (2'-3'-seco-5-methyl-6-AC, 2',3'-dideoxy-2',3'-didehydro-6-AC and 2'-deoxy-6-AC) on the model of Epstein-Barr virus in Raji lymphoblastoid cells.

Synthesis and biological activity of the nucleoside analogs based on polyfluoroalkyl-substituted 1,2,3-triazoles.

This paper offers the results of a synthesis and study of cytotoxicity and the anti-Epstein-Barr virus (EBV) activity of new 2-deoxy-2-chloro-pyranosyl derivatives of 4-tosyl-5-trifluoromethyl-1,2,3-triazole obtained via the addition reaction of the corresponding 2-N-chlorotriazole to the double bond of 3,4,6-tri-O-acetyl-D-glucal. Nucleoside mimetics, derivatives of 4-tosyl-5-polyfluoroalkyl-1,2,3-triazoles containing fragments of 3-chloro-tetrahydrofuran, 3-chloro-tetrahydropyran, tetrahydropyran, dihydrofuran, dihydropyran, or acyclic substituents, were also studied. Evaluation of cytotoxicity (trypan blue and MTT methods) and anti-EBV activity (polymerase chain reaction (PCR) method) showed high selectivity indices for the compounds 4a, 4b, 5b, 6, and 8.

[Effect of isonicotinic acid derivates on reproduction of Epstein-Barr virus].

Current approaches to the treatment of herpes infection, particularly Epstein-Barr virus (EBV), include the use of etiotropic medicines, as well as sensitizing therapy. This virus plays an important role in the etiology of nasopharyngeal carcinoma, adenocarcinoma of the parotid glands, gastric carcinoma, Burkitt's lymphoma and lymphoproliferative syndromes [1, 2, 3]. The spectrum of drugs active against EBV remains very limited, and gancyclovir and acyclovir are used in medical practice, so the search of new compounds active against EBV remains urgent.

Inhibitors of Epstein-Barr virus reproduction--ribonucleosides of 3-substituted 1,2,4-triazino[5,6-b] [1,4] benzothiasines.

Studying of antiviral effect of condensed triazine derivatives--3-oxo- triazinobenzothiazine (the preparation No 1), its ribonucleoside (the preparation No 3) and ribonucleoside of its 3-thio-analogue (the preparation No 2) on reproduction of Epstein-Barr virus (EBV) in lymphoblastoid Raji cells is reported in present work. The cytotoxic action of these preparations on test cells was determined and CC50 index was 125 microg/ml for the preparation No 1, for preparation No 2 - 625 microg/ml, and for preparation No 3 - 750 microg/ml. Antiviral activity of the preparations was tested by inhibition level of accumulation of viral DNA in the cell culture, and effective concentration (EC50 index) was determined for each preparation.

Physico-chemical and biological properties of solvent/detergent treated immunoglobuin G preparations.

Protein composition homogeneity, fraction composition, molecular weight composition, anticomplementary activity, anti-A and anti-B haemagglutinins, specific activity, and pyrogens were studied in intramuscular and intravenous immunoglobulin G preparations, treated and non-treated with solvent/detergent mixture for virus inactivation. Physico-chemical and biological properties retention is shown. Quality of immunoglobulin preparations was improved due to purification by the methods of anion- and cation- exchange preparative low pressure liquid chromatography.

[Effect of proteflazid on the Epschtein-Barr virus].

Cytotoxicity and anti-EBV activity ofProteflasid (SPC Ekopharm, Kyiv) in two solvents: propylene glycol (PG) and syrup--in the culture of lymphoblastoid Raji cells were studied. It was determined that cytotoxic concentration (CC50) of Proteflasid in propylene glycol was 40 microg/ml, and in syrup--150 microg/ ml; effective concentration (EC50) of Proteflasid was equal for both solvents and was 0.1 microg/ml (under therapeutic action of Proteflasid) and 0.

Elaboration of optical immunosensors based on the surface plasmon resonance for detecting specific antibodies and antigens of Epstein-Barr virus and human adenovirus.

The study of antigen-antibody interaction on the model of Epstein-Barr virus (EBV) and second type adenovirus (Ad2) based on the surface plasmon resonance (SPR) was carried out. Kinetic and concentration dependences between virus antigens and specific antisera to them at different pH were determined. Experimental samples of biosensors for the detection by SPR method of virus (EBV and Ad2) antigens using monospecific antibodies, immobilized on the surface of gold, and also for detection of specific antibodies in the blood sera of patients with EBV or adenovirus infection were elaborated

[Modern tendencies in the study of human viruses].

The results of fundamental and applied researches of the Department of Molecular Biology of Viruses and Virus Reproduction Laboratory created on its basis, for the recent five years are generalized in this paper. The basic trends of research of fundamental regularities of virus-cell interaction, search of the anti-virus substances, new approaches to diagnosis of human viruses were analyzed. The spectrum of practical development of the Laboratory, which includes preparations for diagnostics and treatment of human diseases, is presented.

[Use of the surface plasmon resonance method for the virological investigations].

The literature data of the last decade concerning the use of the surface plasmon resonance method for detecting viral proteins as well as intact viruses has been cited. Also the principle of the method was stated and peculiarities of its use for the virus investigations considered. The conclusion was drawn about rich potentialities of the SPR method for investigation of different aspects of the virus interactions with specific agents as well as structure changes provoked by different external factors.

[Research of modulation of CD95-mediated apoptosis in lymphoblastic MP-1 and BJAB cells infected by adenovirus and Epstein-Barr virus].

Model systems of infecting limphoblastic MP-1 and BJAB cells by Epstein-Barr virus, 5 serotype adenovirus and double infection are developed. A rather high level of accumulation of DNA of these viruses in the cells in dynamics at monoinfection and inhibition interference at multi-infection was shown by PCR method. The influence of virus infection on proliferative activity was studied.

[Using the preparation "human immunoglobulin against herpes simplex virus type 1 for intramuscular injections" in the complex therapy of nervous system diseases].

The technology of obtaining of specific immunoglobulin for serotherapy of neuroinfection caused by virus herpes simplex 1 type was developed. The patients presented with the following diseases: arachnoencephalitis, encephalopolyradiculoneuritis, encephalomyelitis, encephalitis, arachnoiditis, polyneuropathy, encephalomyelopolyradiculoneuritis, meningoencephalitis. The study showed good tolerance and safety of the medicine, no adverse effects registered during the study.

[Specific immunoglobulins as effective drugs for treatment of virus infections: present state and urgent tasks in Ukraine].

The thesis about importance of specific immunoglobulin preparations in treatment of infectious diseases of viral and microbial etiology, uncontrollable infections, caused by representatives of herpes viruses family in particular, is developed in the paper. The types of immunoglobulin preparations, their advantages, technology peculiarities has been considered; the experience of application, including that of the authors have been analyzed. The necessity and expedience of intensive development of this branch of immunobiotechnology in Ukraine is proved, especially in connection with a high level of infectious morbidity in this country, concrete recommendations are given.

[Construction of the immunoenzyme test system "IFA-AtEBV-Strip" for detecting antibodies of the Epstein-Barr virus].

The first home immunoenzyme test-system for finding antibodies to the Epstein-Barr virus (EBV) has been created. The test-system laboratory prototype was called "IFA-AtEBV-strip". Production technology has been developed for specific components of immunoenzyme test system and all the analysis stage conditions have been optimized: the antigen sorption on plates, its interaction with the studied serum, finding out of the formed immune complex.

Therapeutical effect of modified adamantane copolymer compounds: study of molecular mechanisms.

Copolymers of N-polyvinylpyrrolidone-acrylic acid (AB-1) and adamantane derivatives are known to possess marked antiviral activity in in vitro and in ovo models. Among the constructed preparations of AB-1 modified by adamantane derivatives some, especially AB-4 (modified by deitiforin), were found to show more extended antiviral activity and to inhibit markedly virus reproduction in susceptible permissive cell cultures and chicken embryos. In AB-4 treated cells and allantoic sacs, virus titers (influenza virus, herpes virus, and HIV) and virus antigen concentration were decreased.