Comparison of digital and conventional methods of fit evaluation of partial removable dental prosthesis frameworks fabricated by selective laser melting.

Statement Of Problem: Three-dimensional (3D) printing technology may improve the fit of partial removable dental prosthesis frameworks made by selective laser melting. Conventionally, the gaps between definitive casts and prostheses are evaluated by using clinical replicas, but digital evaluations may provide a better alternative.

Purpose: The purpose of this in vitro study was to compare digital and conventional methods for evaluating the fit of partial removable dental prosthesis frameworks made by selective laser melting.

The effect of overnight storage conditions on complete denture colonization by Candida albicans and dimensional stability: A systematic review.

Statement Of Problem: Overnight removal of complete dentures is recommended to reduce the incidence of denture stomatitis. The effect of overnight storage conditions is unclear.

Purpose: The purpose of this systematic review was to assess the effect of overnight storage conditions on complete denture colonization by Candida albicans and to explore the effect of overnight storage conditions on the dimensional stability of complete dentures.

Management of a Class I malocclusion with traumatically avulsed maxillary central and lateral incisors.

This case report describes orthodontic space closure for managing an avulsed maxillary central incisor and a lateral incisor in a growing girl with a Class I deep bite malocclusion with moderate lower and mild upper crowding. The treatment approach moved a central incisor across the midline and substituted a lateral incisor for a central incisor, in combination with canine substitution. Veneers on all maxillary anterior teeth attained acceptable esthetics.

Reliable critical sized defect rodent model for cleft palate research.

Background: Suitable animal models are necessary to test the efficacy of new bone grafting therapies in cleft palate surgery. Rodent models of cleft palate are available but have limitations. This study compared and modified mid-palate cleft (MPC) and alveolar cleft (AC) models to determine the most reliable and reproducible model for bone grafting studies.

Cleft palate reconstruction using collagen and nanofiber scaffold incorporating bone morphogenetic protein in rats.

Background: Absorbable collagen sponge (ACS) loaded with bone morphogenetic protein-2 (BMP-2) is approved for selected clinical applications; however, burst release limits its widespread use. Therefore, nanofiber (NF)-based scaffold with ACS backbone was developed to sustain release of loaded BMP-2 to improve the outcomes of bone grafting in a rodent model of cleft palate.

Methods: BMP-2 was loaded on ACS scaffold and then NF hydrogel with different densities (1-2%) was added to sustain the BMP-2 release.

Modification of human BMSC with nanoparticles of polymeric biomaterials and plasmid DNA for BMP-2 secretion.

Background: Genetic modification of human bone marrow stem cells (hBMSCs) before administration to a patient is emerging as a viable approach to creating tailored cells that perform effectively in a clinical setting. To this end, safe delivery systems are needed that can package therapeutic genes into nanoparticles for cellular delivery.

Methods: We evaluated different plasmids on gene expression and compared the effective plasmids directly in hBMSCs.

Bisphosphonate-decorated lipid nanoparticles designed as drug carriers for bone diseases.

A conjugate of distearoylphosphoethanolamine-polyethylene glycol with 2-(3-mercaptopropylsulfanyl)-ethyl-1,1-bisphosphonic acid (thiolBP) was synthesized and incorporated into micelles and liposomes to create mineral-binding nanocarriers for therapeutic agents. The micelles and liposomes were used to encapsulate the anticancer drug doxorubicin (DOX) and a model protein lysozyme (LYZ) by using lipid film hydration (LFH) and reverse-phase evaporation vesicle (REV) methods. The results indicated that the micelles and LFH-derived liposomes were better at DOX loading than the REV-derived liposomes, while the REV method was preferable for encapsulating LYZ.

Osteogenic differentiation of human mesenchymal stem cells cultured with dexamethasone, vitamin D3, basic fibroblast growth factor, and bone morphogenetic protein-2.

Purpose: Human mesenchymal stem cells (hMSCs) are pursued for cell-based therapies of bone defects. Successful use of hMSCs will require them to be osteogenically differentiated before transplantation. This study was intended to determine the optimal combination(s) of supplements needed for inducing osteogenesis in hMSCs.

In vitro osteogenic induction of human gingival fibroblasts for bone regeneration.

Background And Objective: Periodontitis is an inflammatory disease causing bone loss, and is a primary cause of tooth loss. Gingival fibroblasts are readily available with minimal donor site morbidity and may be ideal for tissue engineering efforts in regenerating lost alveolar bone. Dexamethasone (Dex) is commonly employed for in vitro osteogenic induction of a variety of cells, but its effect on human gingival fibroblasts (HGF) is still controversial.

Anabolic effects of low-intensity pulsed ultrasound on human gingival fibroblasts.

Objective: Low-intensity pulsed ultrasound (LIPUS) demonstrated anabolic effects on cementoblasts, odontoblasts, and periodontal ligament cells. However, LIPUS effect on human gingival fibroblasts (HGF) remains to be investigated. Therefore, we evaluated the in vitro effects of LIPUS on HGF proliferation and differentiation to test its feasibility for periodontal therapy.