Zinc improved erythrocyte deformability and aggregation in patients with beta-thalassemia: An in vitro study.

Background: Thalassemia patients have reduced red cell deformability and decreased plasma zinc levels in their blood.

Objective: This study aimed to evaluate the effects of zinc (Zn) on the hemorheological parameters and antioxidant enzyme activities in β-thalassemia major (TM) and healthy volunteers (HV).

Methods: Hemorheological parameters were measured using LORCA (laser-assisted optical rotational cell analyzer) after adjusting the hematocrit to 40%.

Alterations of erythrocyte rheology and cellular susceptibility in end stage renal disease: Effects of peritoneal dialysis.

In this study, we investigated the effects of peritoneal dialysis on hemorheological and hematological parameters and their relations with oxidant and antioxidant status of uremic patients. Hemorheological parameters (erythrocyte deformability, erythrocyte aggregation, osmotic deformability, blood and plasma viscosity) were measured in patients with renal insufficiency undergoing peritoneal dialysis (PD) and volunteers. Erythrocyte deformability, osmotic deformability and aggregation in both autologous plasma and 3% dextran 70 were measured by laser diffraction ektacytometry.

Acute and long-term effects of hyperbaric oxygen therapy on hemorheological parameters in patients with various disorders.

Inhalation of 100% oxygen in a hyperbaric chamber has been accepted as a useful treatment for patients with various pathologies who suffer from hypoxia. The oxidative effects of hyperbaric oxygen (HBO) on RBCs have been investigated in animals but there is not enough data on hemorheological parameters in patients following HBO treatment (HBOT).In this study, we investigated the effect of HBO on hemorheological and haematological parameters during treatment.

In vitro effects of growth factors and interferon-alpha on busulfan cytotoxicity.

An experimental approach to increasing the effectiveness of leukemia treatment with S-phase-specific cytotoxics is to increase the cycling of leukemia cells with growth factors. However, growth factors may have a different relationship with non-cell-cycle-specific agents. The authors examined the effects of granulocyte colony-stimulating factor (G-CSF), granulocyte/macrophage colony-stimulating factor (GM-CSF), and interferon-alpha (INF-alpha) on the cytotoxic effects of the alkylating agent busulfan on the erythro-myeloid cell line K562.

In vitro effects of vitamin C and selenium on NK activity of patients with beta-thalassemia major.

In this study, the in vitro effects of vitamin C and selenium on natural killer (NK) cell activity of ss-thalassemia major patients was investigated. At first, significant decreased NK activity was found at E:T ratios of 1:1, 5:1, and 10:1 in whole thalassemia patients. Low-dose selenium treatment enhanced NK activity in patients but there was no change in the control group.

Effect of ionic strength of buffer on the measurement of erythrocyte electrophoretic mobility.

Background: Measurement of the electrophoretic mobility (EM) of biological cells provides information on the electrical properties of the cell surface. Different research groups have used different electrophoretic techniques. It is well known that the osmolality of the buffer used during an experiment exerts a major impact on EM, particularly in biological cells.