Background: Enhancing the antibacterial capabilities of dental materials by adding nanoparticles has been the subject of some research. However, the potential toxic effect of this material on the vital tissues should be investigated to avoid additional damage to the tissue.
Objective: This study aimed to validate the long-term cytotoxic and antibacterial properties of an epoxy resin-based endodontic sealer (AH Plus) with and without loading with silver gold nanoparticles (Nano Care Plus Silver Gold®).