Integrating detection and degradation of bisphenol A by photocatalytic fuel cell-driven photoelectrochemical sensor.

To ensure food safety and environmental protection, it is crucial to rapidly identify and remove bisphenol A (BPA), a plasticizer commonly used in the inner lining of food containers and beverage packaging. Here, a photocatalytic fuel cell (PFC)-integrated self-powered photoelectrochemical (PEC) sensor is constructed. Unlike conventional single PEC or PFC sensors, this PFC-integrated PEC sensor relies on not only the difference in Fermi energy levels between photoanode and photocathode but also charge accumulation resulted from the oxidation of BPA by photogenerated holes.

Double-enhanced sandwich electrochemiluminescence aptasensor based on g-CN-Au-luminol nanocomposites and ZnCuS nanosheets for highly sensitive detection of mucin 1.

The traditional luminol electrochemiluminescence (ECL) sensing suffers from low signal response and instability issues. Here, an Au/ZnCuS double-enhanced g-CN-supported luminol ECL aptasensor is constructed for the sensitive detection of human mucin 1 (MUC1). In this platform, g-CN of a large specific surface area is beneficial to load more luminol illuminants.

A simplified molecularly imprinted ECL sensor based on MnSnO nanocubes for sensitive detection of ribavirin.

Conventional single-signal or emerging sandwich-type double-signal electrochemiluminescence (ECL) immunosensors/aptasensors have offered accurate detection of small molecules, yet suffer from complicated setup, long processing time, and non-reusability. Here, we demonstrate a simplified molecularly imprinted ECL sensor based on MnSnO nanocubes. As an n-type semiconductor, MnSnO has numerous active sites that can capture electrons to accelerate chemical reactions, resulting in enhanced ECL activity and stability.

De Novo Green Fluorescent Protein Chromophore-Based Probes for Capturing Latent Fingerprints Using a Portable System.

Rapid visualization of latent fingerprints, preferably at their point of origin, is essential for effective crime scene evaluation. Here, we present a new class of green fluorescent protein chromophore-based fluorescent dyes (LFP-Yellow and LFP-Red) that can be used for real-time visualization of LFPs within 10 s. Compared with traditional chemical reagents for LFPs, these fluorescent dyes are completely water-soluble, exhibit low cytotoxicity, and are harmless to users.

A copper-platinum nanoplatform for synergistic photothermal and chemodynamic tumor therapy ROS outburst and GSH exhaustion.

A multifunctional nanoplatform is obtained by modifying copper hexacyanoferrate (Cu-HCF) nanozyme with hyaluronic acid (HA) and further loading platinum (Pt) nanoparticles. This Cu-HCF-HA@Pt platform shows peroxidase-like and glutathione oxidase-like dual-enzyme catalytic activities and photothermal properties, enabling synergistic chemodynamic and photothermal tumor therapy. HA binds to the CD44 receptor, which is highly expressed on the exterior surface of tumor cells, endowing the nanoplatform with tumor specificity.

N-doped TiO/TiC-driven self-photocatalytic molecularly imprinted ECL sensor for sensitive and steady detection of dexamethasone.

The conventional luminol-based electrochemiluminescence (ECL) biosensor suffers from hampered signal stability due to the self-decomposition of the HO co-reactant. Here, we propose an N-doped TiO/TiC heterojunction driven self-photocatalytic platform for ECL signal amplification and then combine it with molecular imprinting technology for sensitive and steady detection of dexamethasone (DXM). Unlike traditional cases involving specific catalysts or external electron injection, the initial luminescence of luminol in this new system is utilized as the excitation light of N-doped TiO/TiC photocatalyst to promote the conversation of dissolved oxygen to HO, supplying more co-reactants to improve ECL of luminol in turn.

InO/BiS S-scheme Heterojunction-Driven Molecularly Imprinted Photoelectrochemical Sensor for Ultrasensitive Detection of Florfenicol.

Florfenicol (FF) raises significant human health and environmental concerns due to its toxicity to the hematology system and the potential spread of antibiotic-resistant genes. Here, a highly sensitive molecularly imprinted photoelectrochemical (PEC) sensor, featuring an InO/BiS S-scheme heterojunction, is proposed to detect FF without an external voltage supply. Compared with conventional II-type heterojunctions, S-scheme heterojunctions efficiently promote carrier separation and enhance the redox capability of the photocatalytic system.

Triple-Mode Sensor Coupled by Photoelectrochemical, Electrochromic, and Spectral Signals for Sensitive Visualized Detection of Nonylphenol.

Conventional photoelectrochemical (PEC) biosensors suffer from the difficulty of visualizing rapid detection and limited accuracy due to a single-signal output. Here, we develop a PEC, electrochromic (EC), and spectral (ST) triple-mode platform for the sensitive visualized detection of nonylphenol (NP). First, the reasonably stepped Fermi energy level arrangement between the defective TiO anode and MoO cathode enables a remarkable photocurrent response (Mode 1).

Molybdenum Disulfide-Integrated Iron Organic Framework Hybrid Nanozyme-Based Aptasensor for Colorimetric Detection of Exosomes.

Tumor-derived exosomes are considered as a potential marker in liquid biopsy for malignant tumor screening. The development of a sensitive, specific, rapid, and cost-effective detection strategy for tumor-derived exosomes is still a challenge. Herein, a visualized and easy detection method for exosomes was established based on a molybdenum disulfide nanoflower decorated iron organic framework (MoS-MIL-101(Fe)) hybrid nanozyme-based CD63 aptamer sensor.

Self-powered molecular imprinted photoelectrochemical sensing platform of sialic acid employing WO/BiS photoanode and CuInS photocathode.

A dual-photoelectrode molecular imprinted photoelectrochemical (PEC) sensor is first built for the determination of sialic acid (SA) without additional energy supply. Specifically, WO/BiS heterojunction behaves as a photoanode to provide amplified and stable photocurrent for the PEC sensing platform, which is attributed to the matched energy levels of WO and BiS promoting the electron transfer and improving photoelectric conversion properties. CuInS micro-flowers functionalized by molecularly imprinted polymers (MIPs) are served as photocathode to recognize SA, avoiding the deficiency of high production cost and poor stability from biological enzymes, aptamers, or antigen-antibodies.

A sensitive dual-signal electrochemiluminescence immunosensor based on Ru(bpy)@HKUST-1 and CeSnO for detecting the heart failure biomarker NT-proBNP.

A sensitive dual-signal electrochemiluminescence (ECL) immunosensor was proposed based on Ru(bpy)@HKUST-1/TPA and CeSnO/KSO probes for detecting the NT-proBNP biomarker of heart failure. HKUST-1 with a high specific surface area facilitates the loading of more Ru(bpy), effectively improving the anodic signal intensity, while the emerging CeSnO emitter displays a potential-matching cathodic emission with moderate intensity. Two ECL probes were characterized with field emission scanning electron microscopy, X-ray diffraction, XPS, FT-IR spectroscopy and UV-Vis diffuse reflectance spectroscopy.

An endoplasmic reticulum targeting green fluorescent protein chromophore-based probe for the detection of viscosity.

The occurrence of endoplasmic reticulum (ER) stress is the main cause of a variety of biological processes that are closely related to numerous diseases. The homeostasis of the ER microenvironment can be disrupted under ER stress. In this research, by linking a pentafluorophenyl to the green fluorescent protein chromophore, we have developed a new ER-targeting fluorescent probe (GE-Y) for measuring changes of intracellular ER viscosity caused by ER stress.

An ultrasensitive cathodic electrochemiluminescence immunoassay for thrombomodulin based on Ru(bpy) encapsulated by MIL-NH-101(Al) nanocomposites.

The rapid and reliable determination of thrombomodulin (TM) is of great significance for the diagnosis of disseminated intravascular coagulation, thrombosis and others. This work exhibits an electrochemiluminescent (ECL) sensor, which was prepared using Ru(bpy) encapsulated by MIL-NH-101(Al) nanocomposites for the sensitive detection of the new-thrombus marker thrombomodulin (TM) for the first time. Specifically, on one hand, with the advantages of high specific surface area, large hollow porous structure and favorable biocompatibility, MIL-NH-101(Al) could load a large amount of luminescent Ru(bpy) and thereby greatly enhance the ECL signal of the immunosensor.

PDA-coated CPT@MIL-53(Fe)-based theranostic nanoplatform for pH-responsive and MRI-guided chemotherapy.

Theranostic nanoplatforms for multimodal diagnosis and treatment of tumors are a current research hotspot in the field of nanomedicine. MOF-based theranostic nanoplatforms integrating drug delivery with magnetic resonance imaging (MRI) have attracted broad attention in cancer diagnosis and therapy. However, due to the poor chemical and colloidal stability of MOFs, as well as their poor biocompatibility, MOF-based theranostic nanoplatforms still face critical challenges in cancer treatment applications.

A self-powered photoelectrochemical cathodic molecular imprinting sensor based on Au@TiO nanorods photoanode and CuO photocathode for sensitive detection of sarcosine.

Quantitative determination of sarcosine (SAR) in biological liquids is of great importance, as SAR has been recently suggested as a promising biomarker for prostate cancer diagnostics. Herein, a self-powered photoelectrochemical (PEC) molecular imprinted sensor integrated with photoanode (Au@TiO nanorods) and photocathode (CuO) is proposed for the first time towards the specific and sensitive detection of SAR. With the benefits of strong photocurrent driving force attributed to a large inherent deviation between the Fermi levels of photoanode and photocathode in this system, the photogenerated electrons of Au@TiO can rapidly transferred along the outer circuit and attracted by the holes in the valence band of the photocathode, forming a self-powered PEC system and improve the photocurrent of the cathode.

Photoelectrochemical determination of diclofenac using oriented single-crystalline TiO nanoarray modified with molecularly imprinted polypyrrole.

A novel molecular imprint photoelectrochemical (PEC) sensor has been prepared based on oriented single-crystalline TiO nanoarray (TNA) material for sensitive detection of diclofenac (DCF). The TNA obtained by the one-step hydrothermal method was characterized by XRD, SEM, and TEM. Polypyrrole film was formed on the TNA by electrochemical method, and DCF was imprinted on the polymer film as the template molecule.

Integrating potential-resolved electrochemiluminescence with molecularly imprinting immunoassay for simultaneous detection of dual acute myocardial infarction markers.

A novel potential-resolved molecularly imprinted electrochemical luminescence (ECL) immunosensor has been developed for the first time for the dual sensitive detection of markers of acute myocardial infarction (AMI): cardiac troponin I (cTnI) and myoglobin (Mb). In this work, cost-effective and robust molecularly imprinted polymer (MIP) as biomimetic antibody was used to construct the immunosensors through electropolymerization and elution to form polydopamine (PDA)-MIP modified electrode. In the presence of AMI biomarkers, two ECL probes including Ru(bpy)@ MOCs and MoS QDs functionalized by cTnI antibody and Mb aptamer could be specifically captured respectively.

Multifunctional Magnesium Organic Framework-Based Microneedle Patch for Accelerating Diabetic Wound Healing.

Diabetic wound healing is one of the major challenges in the biomedical fields. The conventional single drug treatments have unsatisfactory efficacy, and the drug delivery effectiveness is restricted by the penetration depth. Herein, we develop a magnesium organic framework-based microneedle patch (denoted as MN-MOF-GO-Ag) that can realize transdermal delivery and combination therapy for diabetic wound healing.

A dual-signal electrochemiluminescence immunosensor for high-sensitivity detection of acute myocardial infarction biomarker.

Based on two different types of luminescence systems (Ru﹡(bpy)/TPA and SnO NFs/KSO), a new type of electrochemiluminescence (ECL) immunosensor was prepared, which realized the detection of acute myocardial infarction biomarker cTnI. In this strategy, Ru(bpy), above all, was immobilized on the NH-MIL-125 as a capture probe. Subsequently, cTnI and SnO NFs was bonded to the electrode surface through the interaction between antigen and antibody in turn.

Molecularly imprinted photoelectrochemical sensing based on ZnO/polypyrrole nanocomposites for acrylamide detection.

A highly sensitive quenching molecular imprinting (MIP) photoelectrochemical (PEC) sensor was proposed to detect acrylamide (AM) by using the photoactive composite of ZnO and polypyrrole (PPy) as the PEC signal probe. ZnO, with high electron mobility, excellent chemical and thermal stability as well as good biocompatibility, was selected as the photoelectrically active material. A polypyrrole film was formed on the nanodisk ZnO by electrochemical polymerization, and the recognition site of AM was left on the surface of the PPy film by elution, enabling the specific detection of AM.

A dual-signal electrochemiluminescence immunosensor based on Ru(bpy)@3D-foam graphene and SnS dots for sensitive detection of gastric cancer biomarker CA 72-4.

A novel dual-signal electrochemiluminescence immunosensor with high sensitivity was successfully constructed for the sensitive detection of gastric cancer biomarker CA 72-4. The superior performance of the electrochemiluminescence immunosensor came from the self-calibration function of the dual-signal system "Ru(bpy)@3D-foam graphene/TPA" and "SnS dots/KSO". 3D-foam graphene not only has good electrical conductivity and ideal surface area, but also contains amino groups on its surface, which facilitate electron transfer and can carry a large number of luminous reagents.

A ratiometric electrochemiluminescence resonance energy transfer platform based on novel dye BODIPY derivatives for sensitive detection of lactoferrin.

A ratiometric electrochemiluminescence resonance energy transfer (ECL-RET) platform depended on novel dye BODIPY derivatives was proposed for rapid detection of lactoferrin. This ECL-RET platform is composed of aptamer decorated BODIPY composites and C@BSA, in which BODIPY derivative is the ECL probe and can generate significant resonance energy transfer with KSO. BODIPY derivative and KSO are used as built-in reference signal and calibration signal respectively to eliminate background signal and abnormal change signal by double signal self-calibration process.

Liquid exfoliated biocompatible WS@BSA nanosheets with enhanced theranostic capacity.

Ultrathin transition metal dichalcogenides (TMDs) seem to have a promising future in the field of theranostic agents due to their excellent near-infrared light absorption capacity and large specific surface area. Plenty of previous studies focused on the therapeutic effects of the materials, but were less concerned with the detailed studies of biocompatibility for clinical transformation. In this work, ultrathin WS2 nanosheets coated with bovine serum protein (BSA) (WS2@BSA NSs) were selected as experimental subjects with favorable biocompatibility to explore their potential as a theranostic agent.

Hollow ZnS-CdS nanocage based photoelectrochemical sensor combined with molecularly imprinting technology for sensitive detection of oxytetracycline.

A novel hollow ZnS-CdS nanocage-based molecularly imprinted photoelectrochemical (ZnS-CdS/rMIP PEC) sensor was designed for sensitive detection of oxytetracycline (OTC). O-phenylenediamine was electropolymerized onto hollow ZnS-CdS nanocages to form a polymer film, and then OTC molecules were imprinted on the polymer film through hydrogen bonding. When OTC was eluted, many specific recognition sites were formed on the polymer membrane for detecting OTC in samples.

A new mitochondria-targeting fluorescent probe for ratiometric detection of HO in live cells.

With this research we presented a ratiometric and mitochondria-target fluorescent probe (Mito-HT) for detection of HO both in vitro and in live cells. Mito-HT was constructed by direct conjugation of aryl boronate to fluorophore with three synthetic steps. The borate group is cleaved from Mito-HT in the presence of HO, resulting in the exposure of the hydroxyl group of the electron donating group.