Molecular investigation of infection sources and transmission chains of brucellosis in Zhejiang, China.

In the present study, a total of 7793 samples from 5 different types of hosts were collected and tested, with a seroprevalence of 2.4% (184/7793). Although the seroprevalence of human and animal brucellosis is relatively low, numbers of human brucellosis cases reported have increased continuously from 2004 to 2018.

[Risk Assessment of Snail Output Via the Mud Balls of Transplanted Seedlings in a Nursery Stock Park in Middle Region of Zhejiang Province].

Objective: To investigate the distribution of snails in a nursery stock park in the middle region of Zhejiang Province and assess the risk of snail output via the mud balls of transplanted seedlings, to provide scientific data for making strategies for snail control.

Methods: We selected three species of seedlings including Osmanthus fragrans (a large tree), Camellia sasanqua (a small tree), and Purpus privet (a type of shrub) in a nursery stock park in a snail-positive middle region of Zhejiang Province during 2014-2016 to calculate the areas of regions with snails and the density of living snails. In 30 trees of each species, the distribution of snails within the seedlings ground diameter (radius of investigation, 100 cm for Osmanthus fragrans; 30 cm for Camellia Sasanqua and Purpus Privet) and in different soil layers (surface and superficial layers, 0-3 cm; deep layer, 3-10 cm) was assessed.

[Analysis of cost-effectiveness of molluscacide combined with herbicide by spraying].

Objective: To explore an economical and efficient molluscicidal method suitable for large area of nursery stock field.

Methods: Two nursery stock fields with Oncomelania hupensis were selected as experimental sites, and an experimental group and a control group were set. In the experimental group, the molluscacide and herbicide were alternately used (a purification molluscicidal method) during the period of May to October, 2011.

[Observation of molluscicidal effect of black plastic film combined with carbon amide].

Objective: To evaluate the molluscicidal effect of black plastic film combined with carbon amide.

Methods: In Jiangdong Town, Jinhua City, the field with Oncomelania hupensis in the history was selected as experimental area and divided into 3 groups: Group One was administered with black plastic film combined with carbon amide; Group Two was administered with simple black plastic film; and Group Three was a control group.

Results: On the 3rd, 7th, 15th, 20th and 30th day after the experiment, the mortality rates of 0.

[Cost-effectiveness analysis of mechanical and manual soil-buried methods for Oncomelania hupensis control].

Objective: To explore a high molluscicidal efficient method in special Oncomelania hupensis snail environments.

Methods: In 2005 and 2006, in large special environments (rubble creek beaches and seepage barren hills with snails), the mechanical soil-buried method (excavator digging to bury deep snails) and manual soil-buried method were used respectively, and the results were compared for the cost-effectiveness.

Results: With the mechanical soil-buried method in 2006, the investment was 0.