[Clinical application of composite skin graft consisting of acellular allogenic dermis and autologous skin].

Objective: To study the procedures of applying composite skin graft consisting of acellular allogenic dermis and autologous skin in the treatment of full-thickness burns and scar formation.

Methods: Fifteen burn wounds in 8 patients were treated with the composite skin graft using one-stage grafting.

Results: Complete wound healing was achieved in 13 wounds.

[Effect of protein kinase on endothelial cytoskeleton induced by septic shock].

Objective: To study the effect of cGMP-dependent protein kinase (PKG) on the pathogenesis of septic shock.

Methods: Confluent endothelial cells were disintegrated and centrifugated to obtain cell lysates after being treated with LPS or PKG activator 8-Br-cGMP. PKG activity of lysates was measured with radioactive isotope label method in a reaction system of phosphorylation of specific substrate H2B by PKG, and the shape and the distribution of intracellular filamentous actin were detected by specific fluorescence staining.