Nitrogen-Containing Bisphosphonates Downregulate Cathepsin K and Upregulate Annexin V in Osteoclasts Cultured .

Introduction: Bisphosphonates are widely used in the treatment of osteoporosis; however, they are associated with the serious adverse event of bisphosphonate-related osteonecrosis of the jaw (BRONJ).

Aim: The aim of this study is to assess the effects of nitrogen-containing bisphosphonates (N-PHs) on the synthesis of IL-1, TNF-, sRANKL, cathepsin K, and annexin V in bone cells cultured .

Materials And Methods: Osteoblasts and bone marrow-derived osteoclasts were cultured , subjected to treatment with alendronate, risedronate, or ibandronate at a concentration of 10 M for 0 to 96 h and then assayed for IL-1, sRANKL, and TNF- production by ELISA.