Development of Vaginal Carriers Based on Chitosan-Grafted-PNIPAAm for Progesterone Administration.

Chitosan-based hydrogels possess numerous advantages, such as biocompatibility and non-toxicity, and it is considered a proper material to be used in biomedical and pharmaceutical applications. Vaginal administration of progesterone represents a viable alternative for maintaining pregnancy and reducing the risk of miscarriage and in supporting the corpus luteum during fertilization cycles. This study aimed to develop new formulations for vaginal administration of progesterone (PGT).

Design, Synthesis, In Silico and In Vitro Studies for New Nitric Oxide-Releasing Indomethacin Derivatives with 1,3,4-oxadiazole-2-thiol Scaffold.

Starting from indomethacin (IND), one of the most prescribed non-steroidal anti-inflammatory drugs (NSAIDs), new nitric oxide-releasing indomethacin derivatives with 1,3,4-oxadiazole-2-thiol scaffold (NO-IND-OXDs, ) have been developed as a safer and more efficient multitarget therapeutic strategy. The successful synthesis of designed compounds (intermediaries and finals) was proved by complete spectroscopic analyses. In order to study the interaction of NO-IND-OXDs with cyclooxygenase isoenzymes, a molecular docking study, using AutoDock 4.

New nitric oxide-releasing indomethacin derivatives with 1,3-thiazolidine-4-one scaffold: Design, synthesis, in silico and in vitro studies.

In this study we present design and synthesis of nineteen new nitric oxide-releasing indomethacin derivatives with 1,3-thiazolidine-4-one scaffold (NO-IND-TZDs) (6a-s), as a new safer and efficient multi-targets strategy for inflammatory diseases. The chemical structure of all synthesized derivatives (intermediaries and finals) was proved by NMR and mass spectroscopic analysis. In order to study the selectivity of NO-IND-TZDs for COX isoenzymes (COX-1 and COX-2) a molecular docking study was performed using AutoDock 4.

Chitosan-Graft-Poly(N-Isopropylacrylamide)/PVA Cryogels as Carriers for Mucosal Delivery of Voriconazole.

The objective of this study was to prepare and characterize physically crosslinked gel formulations of chitosan (CS)-graft-poly(N-isopropyl acrylamide) (PNIPAAm) and polyvinyl alcohol (PVA) for smart delivery of an antifungal drug, Voriconazole, for mucosal applications. For this purpose, cryogels of CS--PNIPAAm/PVA and CS/PVA were tested by means of texture profile analysis and rheology to determine optimal matrix properties for topical application. The ratio of 75/25 / % CS--PNIPAAm/PVA was selected to be used for formulation since it gave low compressibility and hardness (1.

Toxicity, Biocompatibility, pH-Responsiveness and Methotrexate Release from PVA/Hyaluronic Acid Cryogels for Psoriasis Therapy.

Poly(vinyl alcohol)/hyaluronic acid cryogels loaded with methotrexate were studied. The physical⁻chemical characterization of cryogels was performed by FT-IR spectroscopy, scanning electron microscopy, differential scanning calorimetry and dynamic mechanical thermal analysis. Acute toxicity and haematological parameters were determined by "in vivo" tests.

Fast RP-HPLC Method for the Determination of Bisoprolol.

Aim: To develop and validate a fast, robust, isocratic reversed-phase high performance liquid chromatographic method for the determination of bisoprolol in bulk and pharmaceutical formulations.

Material And Method: Optimum separation of bisoprolol was achieved using as stationary phase a Zorbax SB-C18 column (100×3 mm; 5µm). The mobile phase was a mixture of phosphate buffer (pH = 3.

A NEW SPECTROPHOTOMETRIC METHOD FOR THE ASSAY OF LISINOPRIL.

Aim: A new spectrophotometric method for the assay of lisinopril using 2,4-dinitrophenol as reagent is described.

Material And Methods: The method involved the addition of 2,4-dinitrophenol to lisinopril in methanol followed by absorbance measurement at 400 nm. Experimental conditions that provide wide linear range, maximum sensitivity, selectivity, accuracy and precisions were optimized.

ANTINFLAMMATORY ACTIVITY OF AN N, N'-DISALICYLIDENEMETHYLENDIAMINE-DERIVED SCHIFF BIS BASE AND ITS COPPER(II) COMPLEX.

Aim: The cooper(II) complex combination of N, N'-disalicylidenemethylenediamine and the Schiff bis base were investigated for anti-inflammatory activity.

Material And Methods: In vivo, the anti-inflammatory activity of the metallic complex in comparison with the activity of the Schiff bis base was tested by the method of Winter and co-workers using the Levy technique.

Results And Discussions: Our study on the anti-inflammatory activity of a new Schiff bis base and its complex cooper(II) combination showed that the Schiff bis bases exhibited significant anti-inflammatory action in acute experimental inflammation when compared to the control group.

Effect of apitherapy products against carbon tetrachloride-induced toxicity in Wistar rats.

The present paper aimed to evaluate the influence of apitherapy diet in Wistar rats with carbon tetrachloride induced hepatotoxicity, by the means of biochemical determinations and histopathological changes of liver, spleen, pancreas and testicular tissue. The experiment was carried out on six groups of male Wistar rats. Hepatic lesions were induced by intraperitoneal injection of carbon tetrachloride (dissolved in paraffin oil, 10% solution), 2 mL per 100 g, every two days, for two weeks.

Spectrophotometric method for estimation of bisoprolol fumarate in tablets.

Unlabelled: Bisoprolol fumarate is prescribed for the treatment of hypertension and angina pectoris.

Aim: The purpose of this study was to develop a simple, sensitive, accurate, and reproducible method for estimation of bisoprolol fumarate in tablets.

Material And Methods: The proposed method was based on a yellow colored complex formed with tropaeolin 00, extractable in dichloromethane with maximum absorbance at 412 nm.

Validation of a spectrophotometric assay method for bisoprolol using picric acid.

Unlabelled: Bisoprolol is a drug belonging to beta blockers drugs used primarily for the treatment of cardiovascular diseases.

Aim: A spectrophotometric method for quantitative determination of bisoprolol was developed based on the formation of a complex combination between bisoprolol and picric acid.

Material And Methods: The complex combination of bisoprolol and picric acid has a maximum absorbance peak at 420 nm.

Spectrometric method for the assay of ramipril using molybdophosphoric acid.

Unlabelled: Ramipril is a drug of the angiotensin converting enzyme inhibitor class.

Aim: A new molecular absorbance spectrometric method was developed for the assay of ramipril using molybdophosphoric acid in acidic medium.

Material And Methods: The reaction product showed a maximum absorbance at 361 nm.

[Construction and characterisation of a selective membrane electrode for the assay of lisinopril].

Unlabelled: In order to analyze lisinopril using a selective membrane electrode, the electro-active compound was obtained and included in the selective membrane. The new analysis method was developed and validated.

Material And Method: The electro-active compound was obtained through the precipitation of lisinopril in acidic media using silicowolframic acid solution.

Evaluation of some pharmaceutical formulations of lisinopril through dissolution testing.

Aim: Lisinopril is a drug of the angiotensin converting enzyme (ACE) inhibitor class that is primarily used in treatment of hypertension, congestive heart failure, heart attacks and also in preventing renal and retinal complications of diabetes. We compared the dissolution profiles of Lisinopril 20 mg tablets (Antibiotice S.A.

A new liquid chromatography-tandem mass spectrometry method for determination of bisoprolol in human plasma samples.

Liquid chromatography (LC) coupled with mass spectrometry (MS) detection is one of the most powerful analytical tools for organic compound analysis. The advantages of using LC/MS methods over HPLC methods include: selectivity, chromatographic integrity, peak assignment, structural information, and rapid method development. In this paper, a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the determination of bisoprolol in human plasma samples, using metoprolol as internal standard and liquid-liquid extraction procedure.

[Quantitative determination of adrenaline by visible spectrophotometric method].

Unlabelled: A sensible and rapid spectrophotometric assay for adrenaline was developed.

Material And Method: The method is based on the reaction with ammonium molybdate and sodium nitrite in acid medium.

Results: The obtained compound is stable, soluble in water and has the absorbtion maximum at 405 nm.

[Analytical application of phosphotungstic acid complexes. Synthesis and characterisation].

Unlabelled: Phosphotungstic acid forms salt type ionic association complexes with a large number of medicinal drugs that contain cation forming proton acceptor moieties.

Material And Method: Ranitidine, nizatidine and famotidine complexes with phosphotungstic acid were synthesized and subsequently characterized by their melting point, water solubility, specific absorbance, UV spectra and IR spectra.

Results: These sparingly soluble complexes were applied for the fabrication of membrane selective electrodes for quantitative potentiometric determination of ranitidine, nizatidine and famotidine.

[Turbidimetric assay of famotidine].

This paper proposes a new method for the quantitative determination of famotidine, based on its reaction with phosphomolybdic acid. The measurements were carried out at wavelength of 400 nm. This method has been validated and it has been applied to the determination of famotidine in pharmaceutical products.

[Membrane-selective electrodes for drug analysis. Note II].

Seven membrane-selective electrodes with PVC matrix and liquid-membrane for N-butylscopolamine with different electroactive materials are presented. These electrodes have a linear response in the concentration range of 10(-6) - 10(-2) M N-butylscopolamine bromide, with a detection limit between 4.58 x 10(-7)M and 1.

[UV spectrophotometric assay of famotidine in combination with picrolonic acid, picrolinate].

Famotidine, belonging to H2-antagonist group, is a compound containing a thiazolic moiety and it is used in peptic ulcer therapy. This paper debates the possibility of developing a new ultraviolet spectrophotometric assessment by using the reaction between famotidine and picrolonic acid. We carried out our determinations at 362 nm, where the absorbency of famotidine - picrolonic acid complex is maximal, and we have established the optimal reaction conditions.

[Schiff base type onno spectrophotometric determination of magnesium].

By condensing hydrazide salicylic acid with o-hydroxy carbonilic compounds it was obtained 2--hydroxy acetophenon--salicyl hidrazina, Schiff base--type ONNO. These Schiff bis bases present a good capacity of complexing the Mg (II) ions. The above mentioned Schiff base forms complex with Mg (II) cation, with maximum absorbance at 386 nm, and molar absorbtivity (epsilon) = 6.

[Membrane-selective electrode for atropine assay].

One membrane-selective electrode with PVC matrix for atropine is described, with atropine tetraphenylborate as electroactive material. This electrode has a linear response in the concentration range of 10(-2)-10(-5) M atropine sulphate, with a detection limit of 3.29 x 10(-6) M.

[Visible spectrophotometric assay of ranitidine].

Ranitidine, belonging to H2-antagonist group, is a compound containing a furanic moiety and is used in peptic ulcer therapy. This paper debates the possibility of developing a new visible spectrophotometric assessment by using the reaction between ranitidine and eosine. We carried out our determinations at 505 nm, where the absorbency of ranitidine-eosine complex is maximal, and we have established the optimal reaction conditions.