Consensus Statement on Brillouin Light Scattering Microscopy of Biological Materials.

Brillouin Light Scattering (BLS) spectroscopy is a non-invasive, non-contact, label-free optical technique that can provide information on the mechanical properties of a material on the sub-micron scale. Over the last decade it has seen increased applications in the life sciences, driven by the observed significance of mechanical properties in biological processes, the realization of more sensitive BLS spectrometers and its extension to an imaging modality. As with other spectroscopic techniques, BLS measurements not only detect signals characteristic of the investigated sample, but also of the experimental apparatus, and can be significantly affected by measurement conditions.

Calibration-free quantitative phase imaging in multi-core fiber endoscopes using end-to-end deep learning.

Quantitative phase imaging (QPI) through multi-core fibers (MCFs) has been an emerging in vivo label-free endoscopic imaging modality with minimal invasiveness. However, the computational demands of conventional iterative phase retrieval algorithms have limited their real-time imaging potential. We demonstrate a learning-based MCF phase imaging method that significantly reduced the phase reconstruction time to 5.

AI-driven projection tomography with multicore fibre-optic cell rotation.

Optical tomography has emerged as a non-invasive imaging method, providing three-dimensional insights into subcellular structures and thereby enabling a deeper understanding of cellular functions, interactions, and processes. Conventional optical tomography methods are constrained by a limited illumination scanning range, leading to anisotropic resolution and incomplete imaging of cellular structures. To overcome this problem, we employ a compact multi-core fibre-optic cell rotator system that facilitates precise optical manipulation of cells within a microfluidic chip, achieving full-angle projection tomography with isotropic resolution.

Fully refractive telecentric f-theta microscope based on adaptive elements for 3D raster scanning of biological tissues.

Various techniques in microscopy are based on point-wise acquisition, which provides advantages in acquiring sectioned images, for example in confocal or two-photon microscopy. The advantages come along with the need to perform three-dimensional scanning, which is often realized by mechanical movement achieved by stage-scanning or piezo-based scanning in the axial direction. Lateral scanning often employs galvo-mirrors, leading to a reflective setup and hence to a folded beam path.

Multimode Optical Interconnects on Silicon Interposer Enable Confidential Hardware-to-Hardware Communication.

Following Moore's law, the density of integrated circuits is increasing in all dimensions, for instance, in 3D stacked chip networks. Amongst other electro-optic solutions, multimode optical interconnects on a silicon interposer promise to enable high throughput for modern hardware platforms in a restricted space. Such integrated architectures require confidential communication between multiple chips as a key factor for high-performance infrastructures in the 5G era and beyond.

Chromatic aberration correction employing reinforcement learning.

In fluorescence microscopy a multitude of labels are used that bind to different structures of biological samples. These often require excitation at different wavelengths and lead to different emission wavelengths. The presence of different wavelengths can induce chromatic aberrations, both in the optical system and induced by the sample.

Securing Data in Multimode Fibers by Exploiting Mode-Dependent Light Propagation Effects.

Multimode fibers hold great promise to advance data rates in optical communications but come with the challenge to compensate for modal crosstalk and mode-dependent losses, resulting in strong distortions. The holographic measurement of the transmission matrix enables not only correcting distortions but also harnessing these effects for creating a confidential data connection between legitimate communication parties, Alice and Bob. The feasibility of this physical-layer-security-based approach is demonstrated experimentally for the first time on a multimode fiber link to which the eavesdropper Eve is physically coupled.

Quantitative phase imaging through an ultra-thin lensless fiber endoscope.

Quantitative phase imaging (QPI) is a label-free technique providing both morphology and quantitative biophysical information in biomedicine. However, applying such a powerful technique to in vivo pathological diagnosis remains challenging. Multi-core fiber bundles (MCFs) enable ultra-thin probes for in vivo imaging, but current MCF imaging techniques are limited to amplitude imaging modalities.

Real-time complex light field generation through a multi-core fiber with deep learning.

The generation of tailored complex light fields with multi-core fiber (MCF) lensless microendoscopes is widely used in biomedicine. However, the computer-generated holograms (CGHs) used for such applications are typically generated by iterative algorithms, which demand high computation effort, limiting advanced applications like fiber-optic cell manipulation. The random and discrete distribution of the fiber cores in an MCF induces strong spatial aliasing to the CGHs, hence, an approach that can rapidly generate tailored CGHs for MCFs is highly demanded.

Surveillance of few-mode fiber-communication channels with a single hidden layer neural network.

Multi- and few-mode fibers (FMFs) promise to enhance the capacity of optical communication networks by orders of magnitude. The key for this evolution was the strong advancement of computational approaches that allowed inherent complex light transmission to be surpassed, learned, or controlled, reined in by modal crosstalk and mode-dependent losses. However, complex light transmission through FMFs can be learned by a single hidden layer neural network (NN).

Nonlinear microscopy using impulsive stimulated Brillouin scattering for high-speed elastography.

The impulsive stimulated Brillouin microscopy promises fast, non-contact measurements of the elastic properties of biological samples. The used pump-probe approach employs an ultra-short pulse laser and a cw laser to generate Brillouin signals. Modeling of the microscopy technique has already been carried out partially, but not for biomedical applications.

Benchmarking analysis of computer generated holograms for complex wavefront shaping using pixelated phase modulators.

Wavefront shaping with spatial light modulators (SLMs) enables aberration correction, especially for light control through complex media, like biological tissues and multimode fibres. High-fidelity light field shaping is associated with the calculation of computer generated holograms (CGHs), of which there are a variety of algorithms. The achievable performance of CGH algorithms depends on various parameters.

Rapid computational cell-rotation around arbitrary axes in 3D with multi-core fiber.

Optical trapping is a vital tool in biology, allowing precise optical manipulation of nanoparticles, micro-robots, and cells. Due to the low risk of photodamage and high trap stiffness, fiber-based dual-beam traps are widely used for optical manipulation of large cells. Besides trapping, advanced applications like 3D refractive index tomography need a rotation of cells, which requires precise control of the forces, for example, the acting-point of the forces and the intensities in the region of interest (ROI).

Medical Imaging of Microrobots: Toward Applications.

Medical microrobots (MRs) have been demonstrated for a variety of non-invasive biomedical applications, such as tissue engineering, drug delivery, and assisted fertilization, among others. However, most of these demonstrations have been carried out in settings and under optical microscopy, being significantly different from the clinical practice. Thus, medical imaging techniques are required for localizing and tracking such tiny therapeutic machines when used in medical-relevant applications.

In situ measurement of the isoplanatic patch for imaging through intact bone.

Wavefront-shaping (WS) enables imaging through scattering tissues like bone, which is important for neuroscience and bone-regeneration research. WS corrects for the optical aberrations at a given depth and field-of-view (FOV) within the sample; the extent of the validity of which is limited to a region known as the isoplanatic patch (IP). Knowing this parameter helps to estimate the number of corrections needed for WS imaging over a given FOV.

Physical Layer Security in Multimode Fiber Optical Networks.

The light propagation through a multimode fiber is used to increase information security during data transmission without the need for cryptographic approaches. The use of an inverse precoding method in a multimode fiber-optic communication network is based on mode-dependent losses on the physical layer. This leads to an asymmetry between legitimate (Bob) and illegitimate (Eve) recipients of messages, resulting in significant SNR advantage for Bob.

Impulsive stimulated Brillouin microscopy for non-contact, fast mechanical investigations of hydrogels.

The mechanical properties of tissues and cells are increasingly recognized as an important feature for the understanding of pathological processes and as a diagnostic tool in biomedicine. Impulsive stimulated Brillouin scattering (ISBS) is promising to overcome shortcomings of other measurement methods such as invasiveness, low spatial resolution and long acquisition time. In this paper, we present for the first time ISBS measurements of hydrogels, which are model materials for biological samples.

Diffraction-limited axial scanning in thick biological tissue with an aberration-correcting adaptive lens.

Diffraction-limited deep focusing into biological tissue is challenging due to aberrations that lead to a broadening of the focal spot. The diffraction limit can be restored by employing aberration correction for example with a deformable mirror. However, this results in a bulky setup due to the required beam folding.

Self-calibration of lensless holographic endoscope using programmable guide stars.

Coherent fiber bundle (CFB)-based endoscopes enable optical keyhole access in applications such as biophotonics. In conjunction with objective lenses, CFBs allow imaging of intensity patterns. In contrast, digital optical phase conjugation enables lensless holographic endoscopes for the generation of pixelation-free arbitrary light patterns.

Transmission of Multiple Signals through an Optical Fiber Using Wavefront Shaping.

The transmission of multiple independent optical signals through a multimode fiber is accomplished using wavefront shaping in order to compensate for the light distortion during the propagation within the fiber. Our methodology is based on digital optical phase conjugation employing only a single spatial light modulator, where the optical wavefront is individually modulated at different regions of the modulator, one region per light signal. Digital optical phase conjugation approaches are considered to be faster than other wavefront shaping approaches, where (for example) a complete determination of the wave propagation behavior of the fiber is performed.

Wavefront shaping for imaging-based flow velocity measurements through distortions using a Fresnel guide star.

Imaging-based flow measurement techniques, like particle image velocimetry (PIV), are vulnerable to time-varying distortions like refractive index inhomogeneities or fluctuating phase boundaries. Such distortions strongly increase the velocity error, as the position assignment of the tracer particles and the decrease of image contrast exhibit significant uncertainties. We demonstrate that wavefront shaping based on spatially distributed guide stars has the potential to significantly reduce the measurement uncertainty.

Transmission of independent signals through a multimode fiber using digital optical phase conjugation.

Multimode fibers are attractive for a variety of applications such as communication engineering and biophotonics. However, a major hurdle for the optical transmission through multimode fibers is the inherent mode mixing. Although an image transmission was successfully accomplished using wavefront shaping, the image information was not transmitted individually for each of the independent pixels.

Volumetric HiLo microscopy employing an electrically tunable lens.

Electrically tunable lenses exhibit strong potential for fast motion-free axial scanning in a variety of microscopes. However, they also lead to a degradation of the achievable resolution because of aberrations and misalignment between illumination and detection optics that are induced by the scan itself. Additionally, the typically nonlinear relation between actuation voltage and axial displacement leads to over- or under-sampled frame acquisition in most microscopic techniques because of their static depth-of-field.

Optical dynamic deformation measurements at translucent materials.

Due to their high stiffness-to-weight ratio, glass fiber-reinforced polymers are an attractive material for rotors, e.g., in the aerospace industry.

On the speckle number of interferometric velocity and distance measurements of moving rough surfaces.

The minimum achievable systematic uncertainty of interferometric measurements is fundamentally limited due to speckle noise. Numerical and physical experiments, regarding the achievable measurement uncertainty of Mach-Zehnder based velocity and position sensors, are presented at the example of the laser Doppler distance sensor with phase evaluation. The results show that the measurement uncertainty depends on the number of speckles on the photo detectors.