Differential DNA Extraction from Lateral Flow Immunochromatographic Tests via the EZ1 Advanced XL System.

This differential extraction protocol details the steps for isolating DNA from sample pads used in lateral flow immunochromatographic (LFI) tests, particularly for cases involving mixed biological samples such as semen and menstrual blood, or other evidence related to sexual assault. This procedure utilizes a differential extraction technique applied to sample pads from immunochromatographic tests, where the sample pads serve as the substrate. The method involves two sequential lysis steps to effectively separate non-sperm and sperm fractions, enabling the targeted isolation of distinct cell types for downstream DNA analysis.

Sensorineural hearing loss in stroke: an age-matched retrospective study.

Objective: To compare characteristics between stroke populations with and without sensorineural hearing loss (SNHL) and assess the impact of SNHL on stroke outcome.

Methods: A retrospective study of patients admitted with stroke was carried out. Patients were divided into two groups, where group A were diagnosed with SNHL and group B were without SNHL.

The elements of end-of-life care provision in paediatric intensive care units: a systematic integrative review.

Background: Deaths in paediatric intensive care units (PICUs) are not uncommon. End-of-life care in PICUs is generally considered more challenging than other settings since it is framed within a context where care is focused on curative or life-sustaining treatments for children who are seriously ill. This review aimed to identify and synthesise literature related to the essential elements in the provision of end-of-life care in the PICU from the perspectives of both healthcare professionals (HCPs) and families.

Psychosocial and Support Needs of the Main Caregiver for Adolescents and Young Adults Undergoing Treatment for Cancer.

Background: Family relationships and social networks are critically important to adolescents and young adults (AYAs) with cancer, impacting their experience and well-being throughout the cancer trajectory. A cancer diagnosis impacts the development of independence and an adult identity, which can present challenges to psychosocial well-being needs and relationships between caregivers and AYAs.

Objective: The aim of this study was to explore the psychosocial and support needs of the main caregivers of AYAs.

Moral distress in nurses in developing economies: an integrative literature review.

Background: Moral distress has been extensively studied in developed economies; however, not much in terms of studies has been carried out in developing economies.

Objective: To review the literature reporting the experience of moral distress in nurses in health care settings in developing economies.

Design: An integrative literataure review was used.

A novel pathway of levodopa metabolism by commensal Bifidobacteria.

The gold-standard treatment for Parkinson's disease is levodopa (L-DOPA), which is taken orally and absorbed intestinally. L-DOPA must reach the brain intact to exert its clinical effect; peripheral metabolism by host and microbial enzymes is a clinical management issue. The gut microbiota is altered in PD, with one consistent and unexplained observation being an increase in Bifidobacterium abundance among patients.

Barriers to healthcare and a 'triple empathy problem' may lead to adverse outcomes for autistic adults: A qualitative study.

Autistic people live with more mental and physical health conditions and, on average, die younger than non-autistic people. Despite widespread commitments to tackling these issues, autistic people still report various barriers to accessing healthcare. This article aims to explore the area in depth, from the perspective of autistic people.

Contrasting epistemologies: Biomedicine, narrative medicine and indigenous story medicine.

Background: Narrative Medicine (NM) and Indigenous Story Medicine both use narrative to understand and effect health, but their respective conceptualizations of narrative differ.

Aims: I contrast the concept of narrative in NM with that of Indigenous Story Medicine.

Materials And Methods: The article relies Western narrative theorists as well as Indigenous epistemologists to frame a discussion-by-contrast of the Judeo-Christian creation myth with a Haundenosaunee Creation Story.

Moral distress experienced by neonatal intensive and paediatric care nurses in Northern Ghana: a qualitative study.

Background: Moral distress has been studied widely in nursing but not in developing economies.

Aim: To investigate how moral distress is experienced by nurses working in neonatal intensive care and paediatric wards in Northern Ghana and to determine support measures offered by nurse managers.

Method: Qualitative descriptive method.

As 'synapses' become 'protoplasmic kisses': Symmetry across clinical reasoning and poetry.

Context: We argue that biomedicine at root is not primarily instrumental, but shares aesthetic, ethical and political values with poetry. Yet an instrumentalist bias in medical pedagogy can lead to frustration of biomedicine's potential. Such unfulfilled potential is exposed when making a comparison with poetry, a knowledge system that expressly engages a range of value systems.

A Survey of Canadian Massage Therapists Experiences of Work-Related Pain.

An investigation into the work-related pain (WRP) experienced by Massage Therapists (MTs) in Ontario, Canada was undertaken using voluntary completion of an online questionnaire following broad distribution of requests to take part in this study. Data from respondents were included for analysis if they provided informed consent and were a currently or previously registered MT in the region. With voluntary participation, self-selection respondent bias must be considered in evaluating reported results.

Physicians' perspectives on processes for emergency mental health transfers from university health clinics to hospitals in Ontario, Canada: a qualitative analysis.

Background: In Ontario, Canada, there is variability in how students experiencing a mental health crisis are transferred from university health clinics to hospital for emergent psychiatric assessment, particularly regarding police involvement and physical restraint use. We sought to understand existing processes for these transfers, and barriers to and facilitators of change.

Methods: Between July 2018 and January 2019, we conducted semistructured qualitative interviews by telephone or in person with physicians working at Ontario university health clinics.

Barriers to healthcare and self-reported adverse outcomes for autistic adults: a cross-sectional study.

Objectives: Autistic people experience poor physical and mental health along with reduced life expectancy compared with non-autistic people. Our aim was to identify self-reported barriers to primary care access by autistic adults compared with non-autistic adults and to link these barriers to self-reported adverse health consequences.

Design: Following consultation with the autistic community at an autistic conference, , we developed a self-report survey, which we administered online through social media platforms.

A logical development: biomedicine's fingerprints are on the instrument of close reading in Charonian Narrative Medicine.

Narrative Medicine as originated by Rita Charon began as an attempt to redress the unopposed biomedicalisation of the medical profession. Although the movement has been self-positioned as a corrective to deliver an ideal of care, it began within the rhetorical framework of biomedicine and not outside of it. Thus, Narrative Medicine justifies itself in biomedical terms, invoking instrumental rationales for its use.

Decision-making experiences of health professionals in withdrawing treatment for children and young people: A qualitative study.

Objective: To explore factors that influence professionals in deciding whether to withdraw treatment from a child and how decision making is managed amongst professionals as an individual and as a team.

Study Design: Semi-structured interviews were conducted with a purposive sample of health professionals working at a UK Children's Hospital, with children with life-limiting illnesses whose treatment has been withdrawn. Data were transcribed verbatim, anonymized and analysed using a thematic framework method.

Normobaric Oxygen Therapy in Acute Stroke: A Systematic Review and Meta-Analysis.

Purpose: Normobaric oxygen (NBO) is potentially a readily accessible neuroprotective therapy. We undertook a systematic review to assess NBO in acute stroke.

Methods: MEDLINE, EMBASE, and CENTRAL databases were searched to December 2020.

Sedentary behaviour in non-ambulant children and young people with physical disabilities: a systematic search and review protocol.

Introduction: Non-ambulant children and young people with physical disabilities are at high risk of experiencing negative health outcomes associated with sedentary time. A previous scoping review summarising evidence relating to sedentary behaviours of children with physical disabilities identified the need for validated methods of measuring physical activity of children who use wheelchairs and evaluation of interventions to reduce sedentary time. The scoping review did not assess the quality of evidence relating to this topic, therefore its validity remains unclear.