Recombinant Trichoderma harzianum endoglucanase I (Cel7B) is a highly acidic and promiscuous carbohydrate-active enzyme.

Trichoderma filamentous fungi have been investigated due to their ability to secrete cellulases which find various biotechnological applications such as biomass hydrolysis and cellulosic ethanol production. Previous studies demonstrated that Trichoderma harzianum IOC-3844 has a high degree of cellulolytic activity and potential for biomass hydrolysis. However, enzymatic, biochemical, and structural studies of cellulases from T.

Biosurfactant production by rhodococcus erythropolis and its application to oil removal.

The influence of different nutrients on biosurfactant production by Rhodococcus erythropolis was investigated. Increasing the concentration of phosphate buffer from 30 up through 150 mmol/L stimulated an increase in biosurfactant production, which reached a maximum concentration of 285 mg/L in shaken flasks. Statistical analysis showed that glycerol, NaNO3, MgSO4 and yeast extract had significant effects on production.

Biosurfactant production by Rhodococcus erythropolis grown on glycerol as sole carbon source.

The production of biosurfactant by Rhodococcus erythropolis during the growth on glycerol was investigated. The process was carried out at 28 degrees C in a 1.5-L bioreactor using glycerol as carbon source.

RSM analysis of the effects of the oxygen transfer coefficient and inoculum size on the xylitol production by Candida guilliermondii.

Biotechnology production of xylitol is an excellent alternative to the industrial chemical process for the production of this polyalcohol. In this work the behavior of Candida guilliermondii yeast was studied when crucial process variables were modified. The K(L)a (between 18 and 40/h) and the initial cell mass (between 4 and 10 g) were considered as control variables.

Evaluation of cell recycle on Thermomyces lanuginosus Xylanase A production by Pichia pastoris GS 115.

This work aims to evaluate cell recycle of a recombinant strain of Pichia pastoris GS115 on the Xylanase A (XynA) production of Thermomyces lanuginosus IOC-4145 in submerged fermentation. Fed-batch processes were carried out with methanol feeding at each 12 h and recycling cell at 24, 48, and 72 h. Additionally, the influence of the initial cell concentration was investigated.