Publications by authors named "Negar Hosseini"
Article Synopsis
- Research on human cerebellar development has been limited due to the absence of a cell-based model that accurately reflects the cellular diversity and functions of the human cerebellum.
- The authors introduce a new protocol for creating human cerebellar organoids (hCerOs) from pluripotent stem cells, which successfully replicate key features of the fetal cerebellum and allow for the differentiation of major neurons like granule and Purkinje cells within a month.
- This hCerO model provides a valuable tool for studying cerebellar development and related diseases, enabling various assays and long-term cell survival and maturation, making it accessible for technicians with cell culture skills.
Research on human cerebellar development and disease has been hampered by the need for a human cell-based system that recapitulates the human cerebellum's cellular diversity and functional features. Here, we report a human organoid model (human cerebellar organoids [hCerOs]) capable of developing the complex cellular diversity of the fetal cerebellum, including a human-specific rhombic lip progenitor population that have never been generated in vitro prior to this study. 2-month-old hCerOs form distinct cytoarchitectural features, including laminar organized layering, and create functional connections between inhibitory and excitatory neurons that display coordinated network activity.
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- - Genes linked to synaptic function are often associated with autism spectrum disorder (ASD), but the exact role of these genes in early brain development is not well understood.
- - The study focuses on SYNGAP1, a critical ASD risk gene, demonstrating its expression in human radial glia cells and how its deficiency disrupts brain cell organization and maturation.
- - Findings suggest that disorders related to SYNGAP1 might develop through mechanisms unrelated to synapses, emphasizing the importance of examining neurodevelopmental disorder genes across various human cell types and stages.
Ethnopharmacological Relevance: Pistachio hull has traditionally been used to treat peptic ulcer, hemorrhoids, oral and cutaneous wounds.
Aim Of The Study: On the basis of its traditional uses and previous pharmacological reports, a bioassay guided fractionation procedures on pistachio (Pistacia vera L.) hulls was performed to define the fractions and bioactive compound that are responsible for wound healing activity of hulls.
Background: Non-antifungal drugs appear promising in treatment of opportunistic infections of Candida spp. that are often resistant to current antifungals.
Methods: The broth macrodilution method (NCCLS M27-P document) was used to compare the antifungal activity of trifluoperazine, propranolol, and lansoprazole with that of ketoconazole and amphotericin B, using 50 yeast isolates from the GI tract.