Anther Culture in Jatropha curcas L.: A Tree Species.

The obstacles to breeding programs in Jatropha are the long reproductive cycle with a juvenile phase that lasts several months, the highly heterozygous nature of the genome, the large canopy size, and self-incompatibility that is a long-term process which requires multiple cycles of self-pollination to achieve complete homozygosity. In vitro plant tissue culture-based tools such as haploids and doubled haploid techniques can increase the selection efficiency, resulting into selection of superior plants with complete homozygosity in one generation. It bypasses the complications of greenhouse field evaluation or off-season generation advancement, which takes about 8-10 generations in traditional breeding with the time line of 10-12 years.

De Novo Sequencing and Hybrid Assembly of the Biofuel Crop L.: Identification of Quantitative Trait Loci for Geminivirus Resistance.

is an important perennial, drought tolerant plant that has been identified as a potential biodiesel crop. We report here the hybrid de novo genome assembly of generated using Illumina and PacBio sequencing technologies, and identification of quantitative loci for Mosaic Virus (JMV) resistance. In this study, we generated scaffolds of 265.