Waste office paper: A potential feedstock for cellulase production by a novel strain Bacillus velezensis ASN1.

This paper reports the cellulase (FPase) production by newly isolated Bacillus velezensis ASN1 using waste office paper (WOP) as feedstock and optimization of production conditions through two level factorial design, steepest ascent/descent and second order response surface methodology (RSM). Various fermentation parameters, like chemical factors (potassium dihydrogen phosphate, potassium chloride, yeast extract, magnesium sulphate, sodium nitrate, Tween 80, and waste office paper), physical factors (temperature, pH and time) and biological factor (inoculum size) were examined using two level full factorial design to check the key factors significantly affecting the cellulase production. The central composite design (CCD) was used to optimize the vital fermentation parameters, such as carbon (WOP), nitrogen, pH, and inoculum concentration in the medium for achieving higher cellulase production.

Biorefinery production of poly-3-hydroxybutyrate using waste office paper hydrolysate as feedstock for microbial fermentation.

Waste paper, a major fraction of municipal solid waste, has a potential to serve as renewable feedstock for the biorefineries of fuels, chemicals and materials due to rich in cellulose and abundant at low cost. This study evaluates the possibility of waste office paper (WOP) to serve as a potential feedstock for the biorefinery production of poly (3-hydroxybutyrate). In this study, the WOP was pretreated, enzymatically saccharified and the hydrolysate was used for PHB production.

Production of polyhydroxybutyrate from wheat bran hydrolysate using Ralstonia eutropha through microbial fermentation.

The increasing global demand for sustainable resources necessitates the complete utilization of feedstock. Wheat bran consists of significant amount of cellulose and hemicellulose which can be used as a renewable resource for production of fermentable sugars. In this study, alkaline pretreated wheat bran was enzymatically hydrolyzed using cellulase of Trichoderma reesei (37 FPU/g) and β - glucosidase of Aspergillus niger (50 CBU/g).

Production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 and its broad antibacterial spectrum.

Objective: To study the production, purification and characterization of bacteriocin from Lactobacillus murinus AU06 isolated from marine sediments and its broad spectrum of inhibition against fish pathogens.

Methods: The selected strain was used in production, purification and characterized of bacteriocin. In addition, purified bacteriocin was tested for its antimicrobial activity against fish pathogens.

Thermostable, haloalkaline cellulase from Bacillus halodurans CAS 1 by conversion of lignocellulosic wastes.

An extracellular thermostable, haloalkaline cellulase by bioconversion of lignocellulosic wastes from Bacillus halodurans CAS 1 was purified to homogeneity with recovery of 12.54% and purity fold 7.96 with the molecular weight of 44 kDa.

Optimization, purification and characterization of novel thermostable, haloalkaline, solvent stable protease from Bacillus halodurans CAS6 using marine shellfish wastes: a potential additive for detergent and antioxidant synthesis.

A protease producing marine bacterium, Bacillus halodurans CAS6 isolated from marine sediments, was found to produce higher enzyme by utilizing shrimp shell powder. Optimum culture conditions for protease production were 50 °C, pH 9.0, 30 % NaCl and 1 % shrimp shell powder (SSP) and the protease purified with a specific activity of 509.

Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity.

Marine waste is an abundant renewable source for the recovery of several value added metabolites with potential industrial applications. This study describes the production of chitinase on marine waste, with the subsequent use of the same marine waste for the extraction of antioxidants. A chitinase-producing bacterium isolated from seafood effluent was identified as Alcaligenes faecalis AU02.