Synchronised Video, Motion Capture and Force Plate Dataset for Validating Markerless Human Movement Analysis.

The BioCV dataset is a unique combination of synchronised multi-camera video, marker based optical motion capture, and force plate data, observing 15 healthy participants (7 males, 8 females) performing controlled and repeated motions (walking, running, jumping and hopping), as well as photogrammetry scan data for each participant. The dataset was created for the purposes of developing and validating the performance of computer vision based markerless motion capture systems with respect to marker based systems.

Automating Video-Based Two-Dimensional Motion Analysis in Sport? Implications for Gait Event Detection, Pose Estimation, and Performance Parameter Analysis.

Background: Two-dimensional (2D) video is a common tool used during sports training and competition to analyze movement. In these videos, biomechanists determine key events, annotate joint centers, and calculate spatial, temporal, and kinematic parameters to provide performance reports to coaches and athletes. Automatic tools relying on computer vision and artificial intelligence methods hold promise to reduce the need for time-consuming manual methods.

Label-free detection and profiling of individual solution-phase molecules.

Most chemistry and biology occurs in solution, in which conformational dynamics and complexation underlie behaviour and function. Single-molecule techniques are uniquely suited to resolving molecular diversity and new label-free approaches are reshaping the power of single-molecule measurements. A label-free single-molecule method capable of revealing details of molecular conformation in solution would allow a new microscopic perspective of unprecedented detail.

Examination of 2D frontal and sagittal markerless motion capture: Implications for markerless applications.

This study examined if occluded joint locations, obtained from 2D markerless motion capture (single camera view), produced 2D joint angles with reduced agreement compared to visible joints, and if 2D frontal plane joint angles were usable for practical applications. Fifteen healthy participants performed over-ground walking whilst recorded by fifteen marker-based cameras and two machine vision cameras (frontal and sagittal plane). Repeated measures Bland-Altman analysis illustrated that markerless standard deviation of bias and limits of agreement for the occluded-side hip and knee joint angles in the sagittal plane were double that of the camera-side (visible) hip and knee.

Live-cell three-dimensional single-molecule tracking reveals modulation of enhancer dynamics by NuRD.

To understand how the nucleosome remodeling and deacetylase (NuRD) complex regulates enhancers and enhancer-promoter interactions, we have developed an approach to segment and extract key biophysical parameters from live-cell three-dimensional single-molecule trajectories. Unexpectedly, this has revealed that NuRD binds to chromatin for minutes, decompacts chromatin structure and increases enhancer dynamics. We also uncovered a rare fast-diffusing state of enhancers and found that NuRD restricts the time spent in this state.

Neuroimaging, clinical and life course correlates of normal-appearing white matter integrity in 70-year-olds.

We investigate associations between normal-appearing white matter microstructural integrity in cognitively normal ∼70-year-olds and concurrently measured brain health and cognition, demographics, genetics and life course cardiovascular health. Participants born in the same week in March 1946 (British 1946 birth cohort) underwent PET-MRI around age 70. Mean standardized normal-appearing white matter integrity metrics (fractional anisotropy, mean diffusivity, neurite density index and orientation dispersion index) were derived from diffusion MRI.

Label-free observation of individual solution phase molecules.

The vast majority of chemistry and biology occurs in solution, and new label-free analytical techniques that can help resolve solution-phase complexity at the single-molecule level can provide new microscopic perspectives of unprecedented detail. Here, we use the increased light-molecule interactions in high-finesse fiber Fabry-Pérot microcavities to detect individual biomolecules as small as 1.2 kDa with signal-to-noise ratios >100, even as the molecules are freely diffusing in solution.

Timing of physical activity across adulthood on later-life cognition: 30 years follow-up in the 1946 British birth cohort.

Background: To assess how timing, frequency and maintenance of being physically active, spanning over 30 years in adulthood, is associated with later-life cognitive function.

Methods: Participants (n=1417, 53% female) were from the prospective longitudinal cohort study, 1946 British birth cohort. Participation in leisure time physical activity was reported five times between ages 36 and 69, categorised into: not active (no participation in physical activity/month); moderately active (participated 1-4 times/month); most active (participated 5 or more times/month).

A comprehensive assessment of age at menopause with well-characterized cognition at 70 years: A population-based British birth cohort.

Objectives: Associations between age at menopause and cognition post-menopause are examined to determine whether relationships are stronger for certain cognitive domains.

Study Design: Women from the Medical Research Council National Survey of Health and Development and its neuroscience sub-study, Insight 46, were included if they had known age at menopause (self-reported via questionnaire) and complete cognitive outcome data at age 69 (n = 746) or at Insight 46 wave I (n = 197). Multivariable linear regression analyses adjusting for life course confounders were run; interactions with menopause type (natural/surgical) and APOE-ε4 status were examined; and the potential contribution of hormone therapy was assessed.

The development and evaluation of a fully automated markerless motion capture workflow.

This study presented a fully automated deep learning based markerless motion capture workflow and evaluated its performance against marker-based motion capture during overground running, walking and counter movement jumping. Multi-view high speed (200 Hz) image data were collected concurrently with marker-based motion capture (criterion data), permitting a direct comparison between methods. Lower limb kinematic data for 15 participants were computed using 2D pose estimation, our 3D fusion process and OpenSim based inverse kinematics modelling.

Backward Double Integration is a Valid Method to Calculate Maximal and Sub-Maximal Jump Height.

The backward double integration method uses one force plate and could calculate jump height for countermovement jumping, squat jumping and drop jumping by analysing the landing phase instead of the push-off phase. This study compared the accuracy and variability of the forward double integration (FDI), backwards double integration (BDI) and Flight Time + Constant (FT+C) methods, against the marker-based rigid-body modelling method. It was hypothesised that the jump height calculated using the BDI method would be equivalent to the FDI method, while the FT+C method would have reduced accuracy and increased variability during sub-maximal jumping compared to maximal jumping.

Applications and limitations of current markerless motion capture methods for clinical gait biomechanics.

Background: Markerless motion capture has the potential to perform movement analysis with reduced data collection and processing time compared to marker-based methods. This technology is now starting to be applied for clinical and rehabilitation applications and therefore it is crucial that users of these systems understand both their potential and limitations. This literature review aims to provide a comprehensive overview of the current state of markerless motion capture for both single camera and multi-camera systems.

A Comparative Study of High-Contrast Fluorescence Lifetime Probes for Imaging Amyloid in Tissue.

Optical imaging of protein aggregates in living and post-mortem tissue can often be impeded by unwanted fluorescence, prompting the need for novel methods to extract meaningful signal in complex biological environments. Historically, benzothiazolium derivatives, prominently Thioflavin T, have been the state-of-the-art fluorescent probes for amyloid aggregates, but their optical, structural, and binding properties typically limit them to applications. This study compares the use of novel uncharged derivative, PAP_1, with parent Thioflavin T as a fluorescence lifetime imaging probe.

Development, evaluation and application of a novel markerless motion analysis system to understand push-start technique in elite skeleton athletes.

This study describes the development, evaluation and application of a computer vision and deep learning system capable of capturing sprinting and skeleton push start step characteristics and mass centre velocities (sled and athlete). Movement data were captured concurrently by a marker-based motion capture system and a custom markerless system. High levels of agreement were found between systems, particularly for spatial based variables (step length error 0.

The accuracy of several pose estimation methods for 3D joint centre localisation.

Human movement researchers are often restricted to laboratory environments and data capture techniques that are time and/or resource intensive. Markerless pose estimation algorithms show great potential to facilitate large scale movement studies 'in the wild', i.e.

Single-molecule fluorescence detection of a tricyclic nucleoside analogue.

Fluorescent nucleobase surrogates capable of Watson-Crick hydrogen bonding are essential probes of nucleic acid structure and dynamics, but their limited brightness and short absorption and emission wavelengths have rendered them unsuitable for single-molecule detection. Aiming to improve on these properties, we designed a new tricyclic pyrimidine nucleoside analogue with a push-pull conjugated system and synthesized it in seven sequential steps. The resulting -linked 8-(diethylamino)benzo[][1,8]naphthyridin-2(1)-one nucleoside, which we name ABN, exhibits = 20 000 M cm and = 0.

Can Markerless Pose Estimation Algorithms Estimate 3D Mass Centre Positions and Velocities during Linear Sprinting Activities?

The ability to accurately and non-invasively measure 3D mass centre positions and their derivatives can provide rich insight into the physical demands of sports training and competition. This study examines a method for non-invasively measuring mass centre velocities using markerless human pose estimation and Kalman smoothing. Marker (Qualysis) and markerless (OpenPose) motion capture data were captured synchronously for sprinting and skeleton push starts.

Wild-type sTREM2 blocks Aβ aggregation and neurotoxicity, but the Alzheimer's R47H mutant increases Aβ aggregation.

TREM2 is a pattern recognition receptor, expressed on microglia and myeloid cells, detecting lipids and Aβ and inducing an innate immune response. Missense mutations (e.g.

A comprehensive biomechanical analysis of the barbell hip thrust.

Barbell hip thrust exercises have risen in popularity within the biomechanics and strength and conditioning literature over recent years, as a method of developing the hip extensor musculature. Biomechanical analysis of the hip thrust beyond electromyography is yet to be conducted. The aim of this study was therefore to perform the first comprehensive biomechanical analysis the barbell hip thrust.

Fusing Accelerometry with Videography to Monitor the Effect of Fatigue on Punching Performance in Elite Boxers.

Wearable sensors and motion capture technology are accepted instruments to measure spatiotemporal variables during punching performance and to study the externally observable effects of fatigue. This study aimed to develop a computational framework enabling three-dimensional inverse dynamics analysis through the tracking of punching kinematics obtained from inertial measurement units and uniplanar videography. The framework was applied to six elite male boxers performing a boxing-specific punch fatigue protocol.

vLUME: 3D virtual reality for single-molecule localization microscopy.

vLUME is a virtual reality software package designed to render large three-dimensional single-molecule localization microscopy datasets. vLUME features include visualization, segmentation, bespoke analysis of complex local geometries and exporting features. vLUME can perform complex analysis on real three-dimensional biological samples that would otherwise be impossible by using regular flat-screen visualization programs.

A Comparative Photophysical Study of Structural Modifications of Thioflavin T-Inspired Fluorophores.

The benzothiazolium salt, Thioflavin T (ThT), has been widely adopted as the "gold-standard" fluorescent reporter of amyloid Its properties as a molecular rotor result in a large-scale (∼1000-fold) fluorescence turn-on upon binding to β-sheets in amyloidogenic proteins. However, the complex photophysics of ThT combined with the intricate and varied nature of the amyloid binding motif means these interactions are poorly understood. To study this important class of fluorophores, we present a detailed photophysical characterization and comparison of a novel library of 12 ThT-inspired fluorescent probes for amyloid protein (PAPs), where both the charge and donor capacity of the heterocyclic and aminobenzene components have been interrogated, respectively.

Single-molecule visualization of DNA G-quadruplex formation in live cells.

Substantial evidence now exists to support that formation of DNA G-quadruplexes (G4s) is coupled to altered gene expression. However, approaches that allow us to probe G4s in living cells without perturbing their folding dynamics are required to understand their biological roles in greater detail. Herein, we report a G4-specific fluorescent probe (SiR-PyPDS) that enables single-molecule and real-time detection of individual G4 structures in living cells.