Publications by authors named "Nedka G Panayotova"
Article Synopsis
- * They generated over 427 million long-read sequences and found that longer, more accurate sequences yield better transcript detection, while increased read depth enhances quantification.
- * The study suggests that using reference-based tools works best for well-annotated genomes and recommends incorporating extra data to better identify rare transcripts, providing a benchmark for improving transcriptome analysis techniques in the future.
Long-read sequencing has proven the necessity for high-quality genomic assemblies of reference species, including enigmatic ctenophores. Obtaining high-molecular-weight genomic DNA is pivotal to this process and has proven highly problematic for many species. Here, we discuss different methodologies for gDNA isolation and present a protocol for isolating gDNA for several members of the phylum Ctenophora.
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- The Long-read RNA-Seq Genome Annotation Assessment Project (LRGASP) Consortium aimed to evaluate long-read sequencing for analyzing transcripts by generating over 427 million sequences from various species.
- The findings highlighted that longer, accurate sequences yield better transcript identification, while increased read depth enhances quantification accuracy, particularly in well-annotated genomes.
- The study serves as a benchmark for transcriptome analysis strategies and suggests using additional data for detecting rare transcripts or employing reference-free methods.