High-resolution local trabecular strain within trabecular structure under cyclic loading.

Trabecular bone structure is a complex microstructure consisting of rods and plates, which poses challenges for its mechanical characterization. Digital image correlation (DIC) offers the possibility to characterize the strain response on the surface of trabecular bone. This study employed DIC equipped with a telecentric lens to investigate the strain state of individual trabeculae within their trabecular structure by assessing the longitudinal strain of the trabeculae at both the middle and near the edges of the trabeculae.

A novel specimen shape for measurement of linear strain fields by means of digital image correlation.

Strains on the surface of engineering structures or biological tissues are non-homogeneous. These strain fields can be captured by means of Digital Image Correlation (DIC). However, DIC strain field measurements are prone to noise and filtering of these fields influences measured strain gradients.

Decellularized Cartilage Extracellular Matrix Incorporated Silk Fibroin Hybrid Scaffolds for Endochondral Ossification Mediated Bone Regeneration.

Tissue engineering strategies promote bone regeneration for large bone defects by stimulating the osteogenesis route via intramembranous ossification in engineered grafts, which upon implantation are frequently constrained by insufficient integration and functional anastomosis of vasculature from the host tissue. In this study, we developed a hybrid biomaterial incorporating decellularized cartilage extracellular matrix (CD-ECM) as a template and silk fibroin (SF) as a carrier to assess the bone regeneration capacity of bone marrow-derived mesenchymal stem cells (hBMSC's) via the endochondral ossification (ECO) route. hBMSC's were primed two weeks for chondrogenesis, followed by six weeks for hypertrophy onto hybrid CD-ECM/SF or SF alone scaffolds and evaluated for the mineralized matrix formation in vitro.