Characterization of biosurfactants produced by the oil-degrading bacterium Rhodococcus erythropolis S67 at low temperature.

Production of trehalolipid biosurfactants by Rhodococcus erythropolis S67 depending on the growth temperature was studied. R. erythropolis S67 produced glycolipid biosurfactants such as 2,3,4-succinoyl-octanoyl-decanoyl-2'-decanoyl trehalose and 2,3,4-succinoyl-dioctanoyl-2'-decanoyl trehalose during the growth in n-hexadecane medium at 26 and 10 °C, despite the different aggregate state of the hydrophobic substrate at low temperature.

Asymmetric real-time PCR and multiplex melting curve analysis with TaqMan probes for detecting PIK3CA mutations.

The data in this article are related to the research article entitled "Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations" Botezatu et al. [1]. Somatic mutations in the PIK3CA gene ("hot spots" in exons 9 and 20) are found in many human cancers, and their presence can determine prognosis and a treatment strategy.

Optimization of melting analysis with TaqMan probes for detection of KRAS, NRAS, and BRAF mutations.

The TaqMan probes that have been long and effectively used in real-time polymerase chain reaction (PCR) may also be used in DNA melting analysis. We studied some factors affecting efficiency of the approach such as (i) number of asymmetric PCR cycles preceding DNA melting analysis, (ii) choice of fluorophores for the multiplex DNA melting analysis, and (iii) choice of sense or antisense TaqMan probes for optimal resolution of wild-type and mutant alleles. We also determined ΔTm (i.

[Horizontal transfer of catabolic plasmids in the process of naphthalene biodegradation in model soil systems].

The process of naphthalene degradation by indigenous, introduced, and transconjugant strains was studied in laboratory soil microcosms. Conjugation transfer of catabolic plasmids was demonstrated in naphthalene-contaminated soil. Both indigenous microorganisms and an introduced laboratory strain BS394 (pNF142::TnMod-OTc) served as donors of these plasmids.

[Effect of catabolic plasmids on physiological parameters and efficiency of oil destruction by bacteria of the genus Pseudomonas].

The ability of microbial degraders of polycyclic aromatic hydrocarbons to grow at 24 degrees C in liquid mineral medium supplemented with oil as the sole source of carbon and energy was studied. Growth characteristics (CFU) and the level of oil destruction by plasmid-bearing and plasmid-free strains were determined after seven days of cultivation. The presence of catabolic plasmids in the degrader strains, including rhizosphere pseudomonads, was shown to increase cell growth and enhance the level of oil degradation.

[Methods of optimizing and objective criteria for evaluating the efficacy of chemo-surgical treatment of malignant epithelial tumors of the ovaries].

In a group of 811 patients with epithelial malignancies of the ovaries, the diagnostic and therapeutic procedures used were either standard or they involved elements of optimization based on certain objective criteria. The reliability of diagnosis and treatment efficacy in the two subgroups were compared. Stage I-II tumors made up 37.

[Outpatient care of patients with ovarian cancer after primary treatment and the prevention of recurrence].

According to the scheme worked out by the staff of the 1st oncogynecologic department of the Institute, all patients with ovarian cancer in whom primary treatment was effective should be followed up. Clinical and cytologic examination should be carried out every 2 months during the first 1.5-2 years when recurrence is most likely to develop.

[Malignant tumors of the genitalia in girls].

A review of the literature and analysis of 40 cases of genital malignancies girls are presented. 31 patients had ovarian tumor, 2--cervical, 4--vaginal and 3--tumor of the vulva. Most embryonal rhabdomyosarcoma of the vagina or the vulva occurred in patients under 4 whereas ovarian neoplasms (mostly germ and sex cord tumors) were observed at prepubertal and pubertal age.

[Degree of differentiation of ovarian cancer and its clinical significance].

The study was concerned with morphological criteria for assessing the degree of differentiation of ovarian cancer and establishing their relationship with clinical course in 323 patients. The degree of ovarian cancer differentiation was found to influence the course and prognosis considerably.

[Use of the toxin-tissue receptor reaction for detecting toxic substances of the causative agents of acute intestinal diseases].

The possibility of using erythrocytic ganglioside diagnostic reagents (EGDR) for the detection of V. cholerae, E. coli and S.

[Evaluation of the role of chemotherapy adjuvant to surgery in patients with cancer of the ovary, 1st stage].

The paper discusses the results of a retrospective analysis of the data on the evaluation of the efficacy of chemotherapy as an adjuvant to surgery performed in 167 patients with stage I ovarian cancer who were treated at the Institute and Oncological Dispensary in the city of Gomel in 1963-1979. The evaluation was based on the comparison of 5-year survival rates and the frequency of recurrences in the following three study groups: surgery, surgery + chemotherapy and surgery + chemotherapy + prophylactic chemotherapy. It was found that chemotherapy courses given within 2.

[Foremost tasks in the problem of trophoblastic disease].

Among the most urgent problems of trophoblastic disease are: (1) timely diagnosis which may be assured by histological examination of material obtained by cutterage from uterine cavity whenever discharge is tinged with blood; (2) obligatory determination of the marker of the disease-chorionic gonadotropin in urine; and (3) chemotherapy with due account of "factors of risk". All these problems may be solved chiefly by organizational means and, first of all, by setting up endocrinological laboratories in city districts.

[10 years' experience of the activities of the WHO International Cooperative Center for Clinical Study of Ovarian Tumors].

The data are reported on the many-year activities of the WHO Collaborating Centre on clinical study of ovarian cancer with regard to international cooperation of 10 countries. The organization, main trends and extent of the work performed are referred to, which made it possible to give an idea of the state of diagnosis and treatment of ovarian cancer in the world and enriched the experience of scientific cooperation in this field.

[Polychemotherapy in patients with ovarian cancer].

The authors give a prospective randomized clinical estimation of the efficacy of two schedules of polychemotherapy in the complex treatment of 36 patients with far-advanced malignant ovarian tumors and their recurrences. An incomplete remission of various duration was gained in 4/5 of primary patients and in 3/5 of those with recurrences. The studies concerned failed to show any evident advantages of polychemotherapy over monochemotherapy.

[Evaluation of a method of chemosurgical treatment of malignant ovarian tumors].

Retrospectively, an efficacy of the two main variants of the primary combined therapy for serous cystadenocarcinoma was estimated: "surgery+chemotherapy" (552 patients) and "chemotherapy+surgery" (253 patients). Both variants resulted in rather high incidence (83.5% and 81.

[Characteristics of the metastasis of endometrioid cancer of the ovaries into the greater omentum].

The study is based on the authors' personal material (81 patients). It was found that malignant endometrioid ovarian tumors invade metastatically the greater omentum more rarely than serous carcinomas--in 42% according to our findings, versus 60--80% as reported in the literature with regard to serous neoplasms. Metastases of endometrioid carcinoma into the omentum are more frequently observed in patients over 40.