Draft genome sequencing of MBBL3, a probiotic strain isolated from healthy cow milk.

We sequenced the whole genome of MBBL3, isolated from healthy cow milk. The draft genome of MBBL3 is 2,971,195 bp with 95.61× coverage, spanned over 106 contigs.

Corrigendum to "Unveiling distinct genetic features in multidrug-resistant isolated from mammary tissue and gut of mastitis induced mice" [Heliyon 10(5) (February 2024) e26723].

[This corrects the article DOI: 10.1016/j.heliyon.

A preliminary study of gene expression changes in Koalas Infected with Koala Retrovirus (KoRV) and identification of potential biomarkers for KoRV pathogenesis.

Background: Koala retrovirus (KoRV), a major pathogen of koalas, exists in both endogenous (KoRV-A) and exogenous forms (KoRV-A to I and K to M) and causes multiple disease phenotypes, including carcinomas and immunosuppression. However, the direct association between the different KoRV subtypes and carcinogenesis remains unknown. Differentially expressed gene (DEG) analysis of peripheral blood mononuclear cells (PBMCs) of koalas carrying both endogenous (KoRV-A) and exogenous (KoRV-A, B, and C) subtypes was performed using a high-throughput RNA-seq approach.

Computational identification of -derived phytochemicals as potential inhibitors of nonstructural protein 1 (NSP1) in dengue virus serotype-2.

Background: Dengue virus (DENV) infection, spread by mosquitoes, is a significant public health concern in tropical and subtropical regions. Among the four distinct serotypes of DENV (DENV-1 to DENV-4), DENV-2 is associated with the highest number of fatalities worldwide. However, there is no specific treatment available for dengue patients caused by DENV-2.

Draft genome sequencing of multidrug-resistant strains, isolated from dairy cows with clinical mastitis and their farm environment.

We sequenced the genomes of three multidrug-resistant strains namely 11CM-M1, 11CM-F1, and 11CM-S1, isolated from milk, feces, and farm soil samples collected from dairy cows with clinical mastitis. The assembled draft genomes of these strains were approximately 5.6 Mbp, with a GC content of 62.

Draft genome sequencing of multidrug-resistant strains isolated from dairy cows with clinical mastitis and their farm environment.

We report, for the first time, the draft genomes of four multidrug-resistant strains isolated from milk (2M1), feces (2F1 and 2F2), and farm soil (2S1) samples of dairy cows with clinical mastitis. The assembled genomes of these strains were 5.7 Mbp, 62.

Draft genome sequence of BAW48 isolated from arsenic-contaminated tubewell water in Bangladesh.

We report the draft genome of strain BAW48, a bacterium with a genome size of 6,877,653 bp. This genome comprises gene clusters for arsenic conversion, such as arsenic resistance (), arsenite oxidation (), and arsenate reduction (), along with genes for heavy metal and antibiotic resistance.

Draft genome sequence of an arsenotrophic strain isolated from soil in Bangladesh.

We report the draft genome of an arsenotrophic BAS32 isolated from arsenic (As)-contaminated soil in Bangladesh. This genome contains several predicted gene clusters for As-conversion, namely, As resistance (HCsO), arsenite-oxidizing (BA), and arsenate-reducing (RCDAB) gene clusters along with antibiotic resistance genes (ARGs).

Whole-genome sequencing of probiotic strains isolated from raw milk of healthy cows.

We report the draft genomes of two strains MBBL1 and MBBL2, isolated from raw milk of healthy cows. The genome of MBBL1 is 2,681,695 bp with 57.41× coverage, and MBBL2 is 2,681,119 bp with 99.

Pantothenate kinase: A promising therapeutic target against pathogenic species.

Current treatment of clostridial infections includes broad-spectrum antibiotics and antitoxins, yet antitoxins are ineffective against all species. Moreover, rising antimicrobial resistance (AMR) threatens treatment effectiveness and public health. This study therefore aimed to discover a common drug target for four pathogenic clostridial species, , , , and through an core genomic approach.

In-silico study unveils potential phytocompounds in Andrographis paniculata against E6 protein of the high-risk HPV-16 subtype for cervical cancer therapy.

Despite therapeutic advancements, cervical cancer caused by high-risk subtypes of the human papillomavirus (HPV) remains a leading cause of cancer-related deaths among women worldwide. This study aimed to discover potential drug candidates from the Asian medicinal plant Andrographis paniculata, demonstrating efficacy against the E6 protein of high-risk HPV-16 subtype through an in-silico computational approach. The 3D structures of 32 compounds (selected from 42) derived from A.

Foodborne bacteria in milk and milk products along the water buffalo milk chain in Bangladesh.

Controlling foodborne pathogens in buffalo milk is crucial for ensuring food safety. This study estimated the prevalence of nine target genes representing seven critical foodborne bacteria in milk and milk products, and identified factors associated with their presence in buffalo milk chain nodes in Bangladesh. One hundred and forty-three milk samples from bulk tank milk (n = 34), middlemen (n = 37), milk collection centers (n = 37), and milk product shops (n = 35) were collected and analyzed using RT-PCR.

Soil bacteriome diversity and composition of rooftop and surface gardens in urban and peri-urban areas of Bangladesh.

Soil microbiome science, rapidly evolving, predominantly focuses on field crop soils. However, understanding garden soil microbiomes is essential for enhancing food production sustainability in garden environments. This study aimed to unveil the bacteriome diversity and composition in rooftop garden soils (RGS) and surface garden soils (SGS) across urban (Dhaka North and Dhaka South City Corporations) and peri-urban (Gazipur City Corporation) areas of Dhaka Division, Bangladesh.

Draft genome sequencing of strains isolated from bovine mastitis.

We performed whole-genome sequencing of four multidrug-resistant strains isolated from milk (4M1), feces (4F1 and 4F2), and farm soil (4S1) of mastitic dairy cows. The draft genomes of strains 4M1, 4F1, 4F2, and 4S1 were approximately 4.2 Mbp, with 39.

Unveiling the gut bacteriome diversity and distribution in the national fish hilsa (Tenualosa ilisha) of Bangladesh.

The field of fish microbiome research has rapidly been advancing, primarily focusing on farmed or laboratory fish species rather than natural or marine fish populations. This study sought to reveal the distinctive gut bacteriome composition and diversity within the anadromous fish species Tenualosa ilisha (hilsa), which holds the status of being the national fish of Bangladesh. We conducted an analysis on 15 gut samples obtained from 15 individual hilsa fishes collected from three primary habitats (e.

Draft genome sequencing of strains isolated from cow milk.

We sequenced the genomes of strains MBBL4 and MBBL6, isolated from raw milk samples of healthy cows. The draft genomes of the MBBL4 and MBBL6 were 1,896,831 bp and 1,849,397 bp, respectively, and were fragmented into 58 and 42 contigs, with coverages of 118.2× and 128.

Draft genome sequence of isolated from naturally fermented buffalo milk curd.

This study reports the draft genome of strain BSMRAU-M1L5, isolated from artisanal buffalo milk curd in Bangladesh. The draft genome spans 1,776,471 bp, with 50× coverage and 96 contigs.

Unveiling distinct genetic features in multidrug-resistant isolated from mammary tissue and gut of mastitis induced mice.

is one of the major pathogens causing mastitis in lactating mammals. We hypothesized that from the gut and mammary glands may have similar genomic characteristics in the causation of mastitis. To test this hypothesis, we used whole genome sequencing to analyze two multidrug resistant strains isolated from mammary tissue (G2M6U) and fecal sample (G6M1F) of experimentally induced mastitis mice.

Genomic insights into antibiotic resistance genes in strains isolated from artisanal buffalo milk curd in Bangladesh through whole-genome sequencing.

We sequenced the genome of strains BSMRAU-M1L6 and BSMRAU-M1L13 isolated from artisanal buffalo milk curd in Bangladesh. The draft genomes of BSMRAU-M1L6 and BSMRAU-M1L13 are 1,869,891 and 1,890,611 bp, respectively, with 50.0× coverage (both) and 65 and 75 contigs, respectively.

Genomic features and pathophysiological impact of a multidrug-resistant Staphylococcus warneri variant in murine mastitis.

Non-aureus staphylococci (NAS) represent a major etiological agent in dairy animal mastitis, yet their role and impact remain insufficiently studied. This study aimed to elucidate the genomic characteristics of a newly identified multidrug-resistant NAS strain, specifically Staphylococcus warneri G1M1F, isolated from murine feces in an experimental mastitis model. Surprisingly, NAS species accounted for 54.