Utilization of Galerkin finite element strategy to investigate comparison performance among two hybrid nanofluid models.

The utilization of Fourier's law of heat conduction provides the parabolic partial differential equation of thermal transport, which provides the information regarding thermal transport for the initial time, but during many practical applications, this theory is not applicable. Therefore, the utilization of modified heat flux model is to be used. This work discusses the utilization of non-Fourier heat flux model to investigate thermal performance of tri-hybrid nanoparticles mixture immersed in Carreau Yasuda material past over a Riga plate by using Hamilton Crosser and Yamada Ota models considering the variable thermos-physical characteristics.

Protocol for competition and sequencing of mycobacterium isolated from infected guinea pigs.

We describe steps for gDNA isolation from mycobacterium strains isolated from guinea pig lungs. We detail steps for infection of guinea pigs with , followed by growth, gDNA isolation, and whole genome sequencing. We also describe an competition experiment to determine the selective advantage of one strain over another.

Nb/Starch-Doped ZnO Nanostructures for Polluted Water Treatment and Antimicrobial Applications: Molecular Docking Analysis.

Nb/starch-doped ZnO quantum dots (QDs) were prepared by a coprecipitation route. A fixed quantity of starch (st) and different concentrations (2 and 4%) of niobium (Nb) were doped in a ZnO lattice. To gain a better understanding of synthesized nanostructures, a systematic study was carried out utilizing several characterization methods.

Evaluation of the whole proteome to design a novel mRNA-based vaccine against multidrug-resistant .

, a Gram-negative bacterium, is one of the known disease-causing pathogens. It is resistant to ampicillin, macrolides, cephalosporins, cefotaxime, and ceftazidime. The only antibiotic that has been proven to be effective against is gentamicin.

A Cascaded Mutliresolution Ensemble Deep Learning Framework for Large Scale Alzheimer's Disease Detection Using Brain MRIs.

Alzheimer's is progressive and irreversible type of dementia, which causes degeneration and death of cells and their connections in the brain. AD worsens over time and greatly impacts patients' life and affects their important mental functions, including thinking, the ability to carry on a conversation, and judgment and response to environment. Clinically, there is no single test to effectively diagnose Alzheimer disease.

and missense variants implicate defective neurotransmission in early-onset inherited schizophrenias.

Background: Schizophrenia is characterized by hallucinations, delusions and disorganized behaviour. Recessive or X-linked transmissions are rarely described for common psychiatric disorders. We examined the genetics of psychosis to identify rare large-effect variants in patients with extreme schizophrenia.

Association of CDKAL1 gene polymorphism (rs10946398) with gestational diabetes mellitus in Pakistani population.

Background: CDK5 regulatory subunit associated protein 1 like 1 (CDKAL1) encodes a tRNA modifying enzyme involved in the proper protein translation and regulation of insulin production encoded by the CDKL gene. Sequence variations in the CDKAL1 gene lead to the misreading of the Lys codon in proinsulin, resulting in decreased glucose-stimulated proinsulin production. Various polymorphic sequence variants of the CDKAL1 gene such as rs7754840, rs7756992, rs9465871, and rs10946398 are reported to be associated with type 2 diabetes mellitus and gestational diabetes mellitus (GDM) incidence.

Neurodevelopment assessment of small for gestational age children in a community-based cohort from Pakistan.

Background: Children born small for gestational age (SGA) may experience more long-term neurodevelopmental issues than those born appropriate for gestational age (AGA). This study aimed to assess differences in the neurodevelopment of children born SGA or AGA within a periurban community in Pakistan.

Methods: This was a prospective cohort study in which study participants were followed from the pilot Doppler cohort study conducted in 2018.

Comparative investigation of the catalytic application of α/β/γ-MnO nanoparticles synthesized by green and chemical approaches.

Three phases (α, β, and γ) of manganese dioxide (MnO) are successfully stabilized in a single entity for the first time. For this purpose, Citrullus colocynthis (bitter apple) extract is used as a natural surfactant in green synthesis. MnO nanoparticles were synthesized in the presence and absence of plant extracts under the same conditions.

Chitosan and carbon nitride doped barium hydroxide nanoparticles served as dye degrader and bactericidal potential: A molecular docking study.

Chitosan (CS) and different concentration of graphitic carbon nitride (g-CN) (0.02 wt% and 0.04 wt%) doped barium hydroxide (Ba(OH)) nanoparticles (NPs) were harvested through co-precipitation route.

An outcome of emergency vascular surgery performed by general surgeons; our experience in a university hospital of Pakistan and can they substitute vascular surgeons?

Objective: To measure the outcome of emergency vascular surgery performed by general surgeons, and to identify preventable causes of mortality.

Methods: The retrospective study was conducted at the General Surgery Department of Mayo Hospital, King Edward Medical University, Lahore, Pakistan, and comprised data between January 2014 and May 2019 related to cases regardless of age and gender that required emergency vascular surgery after diagnosis by a consultant surgeon at the surgical emergency. The cases were analysed from admission till discharge.

Klippel Feil Syndrome presenting with tricuspid regurgitation and cardiopulmonary distress secondary to dysplastic thoracic cage and spine: A case report.

Background: Klippel Feil syndrome is a rare multifactorial disease that occurs due to a combination of genetic and environmental factors. It is a complex disease that requires lifelong treatment by multidisciplinary teams.

Case Report: We present a case of a 15-year-old girl who presented with fever and shortness of breath and was found to have Klippel Feil Syndrome with a unique presentation of tricuspid regurgitation with cardiopulmonary distress secondary to dysplastic thoracic cage and spine.

Assessment and Bioaccumulation of Heavy Metals in Fish Feeds, Water, and Some Tissues of Cyprinus carpio Cultured in Different Environments (Biofloc Technology and Earthen Pond System).

Fish feed quality is the main determinant of fish flesh quality, so it is important for successful aquaculture. The current study determines the concentration of heavy metals in fish feeds (A and B), water, and their bioaccumulation in gills, liver, and muscle of C. carpio cultured in different environments (biofloc technology and earthen pond systems).

An overview of biomedical applications of choline geranate (CAGE): a major breakthrough in drug delivery.

A number of studies are on the way to advancing the field of biomedical sciences using ionic liquids (ILs) and deep eutectic solvents (DESs) in view of their unique properties and inherent tunability. These significant solvents tend to enhance the physical properties of the drug, increase their bioavailability and promote the delivery of recalcitrant drugs to the body. One such widely investigated tempting multipurpose IL/DES system is choline geranate (CAGE), which has gained significant interest due to its biocompatible and highly potent antiseptic behavior, which also facilitates its sanitizing ability to combat the coronavirus.

Tragacanth gum coating suppresses the disassembly of cell wall polysaccharides and delays softening of harvested mango (Mangifera indica L.) fruit.

Mango is a climacteric fruit and is prone to high perishability. The rapid softening and ripening (due to degradation and disassembly of cell wall polysaccharides) are the major limitations in extending the storability of the harvested mango fruits. Various types of gum-based edible coatings have been reported for the shelf life extension of mango fruits.

Foliar application of ascorbic acid enhances growth and yield of lettuce () under saline conditions by improving antioxidant defence mechanism.

Lettuce (Lactuca sativa L.) production is low because of different environmental stresses. Salt stress significantly reduces lettuce growth and yield.

Significance of Capping Agents of Colloidal Nanoparticles from the Perspective of Drug and Gene Delivery, Bioimaging, and Biosensing: An Insight.

The over-growth and coagulation of nanoparticles is prevented using capping agents by the production of stearic effect that plays a pivotal role in stabilizing the interface. This strategy of coating the nanoparticles' surface with capping agents is an emerging trend in assembling multipurpose nanoparticles that is beneficial for improving their physicochemical and biological behavior. The enhancement of reactivity and negligible toxicity is the outcome.

Photocatalytic and antibacterial activity of graphene oxide/cellulose-doped TiO quantum dots: molecular docking studies.

Graphene oxide (GO) and cellulose nanocrystal (CNC)-doped TiO quantum dots (QDs) were effectively synthesized by employing the co-precipitation method for the degradation of dyes and antimicrobial applications. A series of characterizations, , XRD, FTIR, UV-visible spectroscopy, EDS, FE-SEM, and HR-TEM, was used to characterize the prepared samples. A reduction in PL intensity was observed, while the band gap energy ( ) decreased from 3.

MoS/cellulose-doped ZnO nanorods for catalytic, antibacterial and molecular docking studies.

Cellulose nanocrystals (CNCs) and molybdenum disulphide (MoS) incorporated into ZnO nanorods (NRs) were synthesized a chemical precipitation route at room temperature. All concerned samples were characterized to examine their optical properties, elemental composition, phase formation, surface morphology and functional group presence. The aim of this research was to enhance the catalytic properties of ZnO by co-doping with various concentrations of CNCs and MoS NRs.

China-Pakistani economic corridor project bring the international trade, healthcare, self-efficacy, and social performance facility to Gilgit city, Pakistan.

This paper aims to identify the impact of China-Pakistani economic corridor project bring the international trade, healthcare, self-efficacy, and social performance facility to Gilgit city, Pakistan. A questionnaire survey was carried out on the scholar presently studying at different universities in china. The 50% scholar estimation silk road enhanced, and 11% have no idea about the increasing of the healthcare opportunity in Gilgit.

Green synthesis of coumarin derivatives using Brønsted acidic pyridinium based ionic liquid [MBSPy][HSO] to control an opportunistic human and a devastating plant pathogenic fungus .

An eco-friendly simple protocol has been devised for the preparation of coumarin derivatives using doubly Brønsted acidic task specific ionic liquid (TSIL) as a catalyst. Solvent-free conditions were employed for the reaction of different substituted phenols with β-ketoester in TSIL to produce corresponding substituted coumarin derivatives in good to excellent yields at ambient conditions; at room temperature and with reduced reaction times. The ionic liquid catalyst can be recycled and reused up to five times.

Facile synthesis of starch and tellurium doped SrO nanocomposite for catalytic and antibacterial potential: In silico molecular docking studies.

A chemical co-precipitation route was used to synthesize novel strontium oxide (SrO), SrO-starch composite and various tellurium (Te) concentrations were incorporated in SrO-starch composite. This study aims to enhance the catalytic activities and bactericidal behavior of SrO, SrO-starch composite with different percentage concentrations of Te doping and a fixed amount of starch nanoparticles. XRD affirmed that the dopant contribution was investigated to improve crystallinity.

Mutation screening of the CYP1B1 gene reveals thirteen novel disease-causing variants in consanguineous Pakistani families causing primary congenital glaucoma.

Background: Primary congenital glaucoma (PCG) is a heterogeneous rare recessively inherited disorder prevalent in regions with high consanguinity. Disease phenotype is associated with increased intra ocular pressure and is a major cause of childhood blindness. Sequence variations in Cytochrome P450 1B1 (CYP1B1) gene are a major cause of PCG.

Biallelic variants in CHST3 cause Spondyloepiphyseal dysplasia with joint dislocations in three Pakistani kindreds.

Background: Skeletal dysplasia is a heterogeneous group of disorders. Spondyloepiphyseal dysplasias comprise one subgroup. Deficiency of carbohydrate sulfotransferase 3 has been reported in a small number of patients with recessively inherited spondyloepiphyseal dysplasia with joint dislocation, short stature and scoliosis.

Mutational analysis in sodium-borate cotransporter SLC4A11 in consanguineous families from Punjab, Pakistan.

Aim: To identify the molecular basis of Congenital Hereditary Endothelial Dystrophy CHED caused by mutations in SLC4A11, in the consanguineous Pakistani families.

Methods: A total of 7 consanguineous families affected with Congenital Hereditary Endothelial Dystrophy were diagnosed and registered with the help of ophthalmologists. Blood samples were collected from affected and unaffected members of the enrolled families.