Detection of genomic variants in BRCA1 and BRCA2 across gastric cancer patients using next generation sequencing.

Objectives: To explore the landscape of BRCA1/2 mutations in gastric cancer patients.

Methods: Next-generation sequencing (NGS), Sanger sequencing, reverse transcription quantitative polymerase chain reaction (RT-qPCR), Immunohistochemistry, The Cancer Genome Atlas (TCGA), gnomAD, and DAVID.

Results: With 95% of bases boasting a phred score surpassing 30 and a minimum coverage depth of 500X, our NGS approach ensures high-quality data acquisition.

COMPLETE HEART BLOCK IN PATIENTS PRESENTING WITH ACUTE ANTERIOR WALL MYOCARDIAL INFARCTION.

Background: Complete heart block (CHB) is a relatively common complication in post-acute myocardial infarction (AMI) patients. Data on the incidence and consequences of CHB in patients with ST-segment elevation myocardial infarction (STEMI) are limited. Besides serving as an indicator of widespread myocardial injury, the development of CHB in STEMI is also associated with a higher risk of sudden cardiac death.

Frequency, Risk Factors, and Methods of Deliberate Self-Harm Observed in Medical Students and Doctors in Pakistan.

Objective: To determine the frequency of deliberate self-harm (DSH), risk factors leading to DSH, and the methods adopted by medical students and young doctors to execute DSH.

Method: A cross-sectional study was conducted on medical college students and young doctors aged 18-26 years. After approval by the parent institute's ethical board, different medical students and doctors from Pakistani medical colleges of both male and female populations were recruited through non-probability sampling.

Impact of Teaching Methods on Clinical Reasoning in Forensic Medicine: A Quasi-Experimental Study.

Objective: To compare the effectiveness of flipped classroom and video-assisted learning techniques with didactic lectures in promoting clinical reasoning skills in Forensic Medicine.

Study Design: Quasi-experimental study. Place and Duration of the Study: Department of Forensic Medicine, Dow International Medical College and Dow University of Health Sciences, Karachi, Pakistan, from May to October 2023.

Can large language models provide accurate and quality information to parents regarding chronic kidney diseases?

Rationale: Artificial Intelligence (AI) large language models (LLM) are tools capable of generating human-like text responses to user queries across topics. The use of these language models in various medical contexts is currently being studied. However, the performance and content quality of these language models have not been evaluated in specific medical fields.

Unveiling the unexplored novel signatures for osteoporosis via a detailed bioinformatics and molecular experiments based approach.

Background: Osteoporosis (OP) stands as a prevalent bone ailment affecting the elderly, globally. The identification of reliable diagnostic markers crucially aids OP clinical management.

Methods: Utilizing the GEO database (GSE35959), we acquired expression profiles for OP and normal samples.

Determination of Risk Factors for Nausea and Vomiting in Children After Appendectomy.

Purpose: This study aimed at investigating the risk factors for postoperative nausea and vomiting (PONV) in pediatric patients undergoing appendectomy.

Design: This is a prospective, descriptive, cross-sectional study.

Methods: The study involved 163 children aged 5 to 18 years who underwent appendectomy in the pediatric surgery clinic of a tertiary hospital between December 2022 and June 2023.

Correlation between levels for stress with level of empathy in undergraduate medical students.

Objective: To determine the correlation between levels of stress with level of empathy in all five years of undergraduate medical students of a private medical college in Pakistan.

Methods: This descriptive correlation study was conducted at Al Tibri Medical College, Karachi from 15 June to 14 November 2021. Of the 500 students in the medical school, 408 participants filled out the questionnaires through online Google Forms.

Incorporation of zinc sulfide nanoparticles, Acinetobacter pittii and Bacillus velezensis to improve tomato plant growth, biochemical attributes and resistance against Rhizoctoniasolani.

Green nanobiotechnology and beneficial bacterial strains as biofertilizers are crucial in agriculture to achieve food security. Both these strategies have been individually studied in improving plant resistance against phytopathogens along with enhancing plant productivity. Therefore, objective of this study was to explore the eco-friendly and cost-effective approach of utilizing plant growth promoting and disease suppressing bacterial strains and nanoparticles, individually as well as in combination, as bio-stimulants to improve plant growth, antioxidant defense system, nutrition and yield of tomato.

Biocontrol of Rhizoctonia solani in basmati rice by the application of Lactobacillus and Weissella spp.

Rice is a staple food crop and is a major source of employment and income in the world. However, the attack of fungal disease poses a serious threat to the crop growth and productivity and leads toward yield loses. Therefore, current study was performed to evaluate the biocontrol potential of Lactobacillus and Weissella spp.

Heavy metals contamination in vegetables irrigated with wastewater: a case study of underdeveloping regions of Pakistan.

Irrigation of food crops with untreated wastewater leads to the accumulation of heavy metals in their edible parts. Exposure to toxic elements through consumption of contaminated vegetables even at very low concentration is becoming a serious problem for human health in many countries. This issue of heavy metals contamination needs great attention especially in Pakistan where irrigation by wastewater has become a common practice in urban and periurban areas.

Effects of menthol gum chewing on postoperative nausea, vomiting, and length of hospital stay in children undergoing appendectomy: A randomized controlled trıal.

Purpose: This study aimed to determine the effects of menthol gum chewing on nausea, vomiting, and length of hospital stay after appendectomy in children.

Background: Postoperative nausea and vomiting (PONV) can be induced by general anesthesia. Several drugs are available to reduce the risk of PONV; however, their cost and side effects limit their clinical use.

Food Polyphenols and Type II Diabetes Mellitus: Pharmacology and Mechanisms.

Type II diabetes mellitus and its related complications are growing public health problems. Many natural products present in our diet, including polyphenols, can be used in treating and managing type II diabetes mellitus and different diseases, owing to their numerous biological properties. Anthocyanins, flavonols, stilbenes, curcuminoids, hesperidin, hesperetin, naringenin, and phenolic acids are common polyphenols found in blueberries, chokeberries, sea-buckthorn, mulberries, turmeric, citrus fruits, and cereals.

Toxoplasmosis-Awareness and Knowledge of Pregnant Women in Rural Areas of Malakand Region, Pakistan.

Background: The current study was carried out between October 2017 and October 2018 to explore knowledge, attitudes, practices, and information sources regarding toxoplasmosis among pregnant women in Malakand region, the northwestern part of Pakistan. The current study was carried out between October 2017 and October 2018.

Methods: A structured questionnaire was used to interview the women after taking verbal informed consent.

Forensic Age Estimation of Individuals from Computed Tomography Analysis of Medial Clavicular Epiphysis in Pakistani Population.

Objective: To determine the association of chronological age through ossification of the medial clavicular epiphysis using computed tomography (CT) scan.

Study Design: An Observational study. Place and Duration of the Study: Dow Institute of Radiology, Dow University Hospital, Karachi, Pakistan, from February 2021 till October 2021.

Structural and Functional Characterization of Novel Phosphotyrosine Phosphatase Protein from (Pupal Retina).

A novel pair of protein tyrosine phosphatases in (pupal retina) has been identified. Phosphotyrosyl protein phosphatases (PTPs) are structurally diverse enzymes increasingly recognized as having a fundamental role in cellular processes including effects on metabolism, cell proliferation, and differentiation. This study presents identification of novel sequences of PTPs and their comparative homology modeling from (Dr-PTPs) and complexation with the potent inhibitor HEPES.

The Transmission Dynamics of a Compartmental Epidemic Model for COVID-19 with the Asymptomatic Population via Closed-Form Solutions.

Unlike previous viral diseases, COVID-19 has an "asymptomatic" group that has no symptoms but can still spread the disease to others at the same rate as symptomatic patients who are infected. In the literature, the mass action or standard incidence rates are considered for compartmental models with asymptomatic compartment for studying the transmission dynamics of COVID-19, but the quarantined adjusted incidence rate is not. To bridge this gap, we developed a Susceptible Asymptomatic Infectious Quarantined (SAIQ) model with a Quarantine-Adjusted (QA) incidence to investigate the emergence and containment of COVID-19.

Common Risk Factors Involved In The Development Of Myocardial Infarction In Adults Younger Than 45 Years Of Age.

Background: Pakistan has got a very high prevalence of myocardial infarction (MI). MI presents at pretty young age in this country. The objective of present study was to determine the frequency of common risk factors associated with early development of MI in middle aged adults of less than 45 years.

A novel homozygous truncating variant in PPFIBP1 further delineates PPFIBP1-associated neurodevelopmental disorder.

Neurodevelopmental disorders (NDDs) are classified as a group of disorders affecting function and development of the brain and having wide clinical variability. Herein, we describe two affected individuals segregating a recessive NDD. The affected individuals exhibited phenotypes such as global developmental delay (GDD), intellectual disability (ID), microcephaly and speech delay.

Rhizosphere bacteria associated with Chenopodium quinoa promote resistance to Alternaria alternata in tomato.

Microorganisms can interact with plants to promote plant growth and act as biocontrol agents. Associations with plant growth-promoting rhizobacteria (PGPR) enhance agricultural productivity by improving plant nutrition and enhancing protection from pathogens. Microbial applications can be an ideal substitute for pesticides or fungicides, which can pollute the environment and reduce biological diversity.

An Insight into Animal Glutamate Receptors Homolog of and Their Potential Applications-A Review.

Most excitatory impulses received by neurons are mediated by ionotropic glutamate receptors (iGluRs). These receptors are located at the apex and play an important role in memory, neuronal development, and synaptic plasticity. These receptors are ligand-dependent ion channels that allow a wide range of cations to pass through.