Association of COVID-19 With Emergence of Comorbidities: A Hospital-Based Cohort Study.

Background: In 2019, the emergence of SARS-CoV-2 marked the beginning of the COVID-19 global pandemic, which reached its peak in 2020. Initially designated as a novel coronavirus, SARS-CoV-2 emerged as a respiratory illness and later began causing multi-organ complications in recovered patients.

Methods: This article presents a hospital-based retrospective cohort study conducted via telephone interviews with patients in a tertiary hospital.

Estimation of Prevalence of Misophonia Among High School Students in India.

Objective: Misophonia, an intriguing psychological disorder is characterized by intense emotional responses to specific sounds produced by others, has gained prominence for its distinct attributes and profound impact on individuals' emotional and psychological well-being. This study addresses the scarcity of research on misophonia's prevalence and severity among high school students, aiming to shed light on the unique challenges faced by this demographic in India.

Methods: The study enrolled 597 high school students, including 269 females and 328 males, aged 14 to 16, with no history of psychological or otological problems or medication use.

Sinococuline, a bioactive compound of Cocculus hirsutus has potent anti-dengue activity.

Dengue virus (DENV) infection has increased worldwide, with over 400 million infections annually, and has become a serious public health concern. Several drug candidates, new and repurposed, have failed to meet the primary efficacy endpoints. We have recently shown that Aqueous Extract of the stem of Cocculus hirsutus (AQCH) was effective in vitro and in vivo against DENV and was safe in humans.

Pathophysiologic approach to understanding and successfully treating idiopathic edema: Unappreciated importance of nocturia.

Idiopathic edema (IE), a disorder of females, is characterized by edema and weight gains exceeding 1.4 kg while assuming an upright position followed by nocturia and returning to a non-edematous baseline weight in the morning. There is no successful treatment of IE and the importance of nocturia needs to be emphasized.

-Derived Phytopharmaceutical Drug Has Potent Anti-dengue Activity.

Dengue is a serious public health concern worldwide, with ∼3 billion people at risk of contracting dengue virus (DENV) infections, with some suffering severe consequences of disease and leading to death. Currently, there is no broad use vaccine or drug available for the prevention or treatment of dengue, which leaves only anti-mosquito strategies to combat the dengue menace. The present study is an extension of our earlier study aimed at determining the and protective effects of a plant-derived phytopharmaceutical drug for the treatment of dengue.

The association between serum chloride levels and chronic kidney disease progression: a cohort study.

Background: Limited data suggest serum chloride levels associate with mortality in heart failure, chronic kidney disease (CKD), and pulmonary arterial hypertension. Randomized trials have also shown that administration of crystalloid intravenous fluids with lower chloride concentration may have better renal outcomes. However, chloride has not been studied longitudinally for CKD progression.

Exploring cellular behavior under transient gene expression and its impact on mAb productivity and Fc-glycosylation.

Transient gene expression (TGE) is a methodology employed in bioprocessing for the fast provision of recombinant protein material. Mild hypothermia is often introduced to overcome the low yield typically achieved with TGE and improve specific protein productivity. It is therefore of interest to examine the impact of mild hypothermic temperatures on both the yield and quality of transiently expressed proteins and the relationship to changes in cellular processes and metabolism.

High-throughput quantitation of Fc-containing recombinant proteins in cell culture supernatant by fluorescence polarization spectroscopy.

Measurement of recombinant protein product titer critically underpins all biopharmaceutical manufacturing process development, as well as diverse research and discovery activity. Here, we describe a simple rapid (<2 min per 96 samples) 96-well microplate-based assay that enables high-throughput quantitation of recombinant immunoglobulin G and Fc-containing IgG derivatives in mammalian cell culture supernatant over a wide dynamic range of 2.5-80 mg/L, using microplate fluorescence polarization (FP) spectroscopy.

Evaluation of strategies to control Fab light chain dimer during mammalian expression and purification: A universal one-step process for purification of correctly assembled Fab.

Fabs are an important class of antibody fragment as both research reagents and therapeutic agents. There are a plethora of methods described for their recombinant expression and purification. However, these do not address the issue of excessive light chain production that forms light chain dimers nor do they describe a universal purification strategy.

Seasonal changes in affective state measured prospectively and retrospectively.

Background: The study was designed to investigate whether seasonal mood and behavioural changes are detectable prospectively in a non-clinical population in the way they have been reported in retrospective studies. The specificity of any seasonal fluctuation in affective state was also investigated by measuring anxiety as well as depression.

Method: To measure seasonal fluctuations in affect and behaviour prospectively, 25 women were interviewed every month for one year using four scales (depression, anxiety, stress, and behavioural change).

Prevalence of genital pathogens among female prostitutes in New York City and in Rotterdam.

The authors studied the prevalence of genital microorganisms among 300 female prostitutes in brothels in New York City and 60 female prostitutes attending a sexually transmitted diseases clinic in Rotterdam, The Netherlands. Rates of isolation of Neisseria gonorrhoeae, Chlamydia trachomatis, Mycoplasma hominis, and Ureaplasma urealyticum in the two cities were 9.3% and 8.

Infections with non-penicillinase-producing Neisseria gonorrhoeae treated with cefuroxime: treatment failures.

Two men who acquired acute gonococcal urethritis in The Netherlands and who were treated with cefuroxime (1.5 g given intramuscularly) failed to respond to this therapy. Pre- and post-treatment isolates of Neisseria gonorrhoeae from these patients had MICs of 0.

[Ano-rectal diseases observed at a venereal disease polyclinic].

The writers show how the polyclinic for venereal diseases at the Djkzigt hospital in Rotterdam is run. Their statistical study shows how they were struck by the large number of pederasts, and by the frequent occurrence of dermatological diseases amongst the various proctological lesions; e.g; acuminate condylomata.

Antibiotic sensitivities of gonococci isolated in Rotterdam and results of treatment with cefuroxime.

In a study of the efficacy of cefuroxime in the treatment of 278 cases of acute gonorrhoea (including rectal and pharyngeal infections and infections due to penicillinase-producing Neisseria gonorrhoeae), high cure rates were found for both male (98.8%) and female patients (98.6%).

Gonorrhea in Rotterdam caused by penicillinase-producing gonococci.

Recently the prevalence of strains of penicillinase-producing Neisseria gonorrhoeae (PPNG) in the Netherlands has increased. A study of demographic and clinical data from patients with gonorrhoea due to PPNG strains, and of the biological characteristics of the strains isolated in Rotterdam, show that Asian strains of PPNG seem at present to be endemic in the Netherlands. Spectinomycin was found to be effective in the treatment of patients with gonorrhoeea due to PPNG strains.

Rising incidence of chancroid in Rotterdam. Epidemiological, clinical, diagnostic, and therapeutic aspects.

The incidence of chancroid in Rotterdam has increased by more than five-fold during 1977-78. In a retrospective study of 53 patients with chancroid seen at this clinic during this period, the results of smears were positive in 82% and of cultures in 84% (of those for whom cultures had been performed). Symptoms were generally mild.

Clotrimazole (Canesten) in the treatment of candidal balanitis in men. With incidental observations on diabetic candidal balanoposthitis.

Clotrimazole 1% (Canesten) cream was used in a trial comprising 138 men with candidal balanitis. Eighty-six (91%) out of 94 men were asymptomatic after seven days and 57 (98%) of 58 men were asymptomatic after three weeks' treatment. After seven days, Candida albicans could no longer be cultured from 86 (90%) of 96 men, nor from 55 (95%) of 58 men three weeks after treatment.

Infection of the uterine cervix with Chlamydia trachomatis.

For identification of those variables in the history and in the clinical and routine laboratory examination that are most likely to indicate infection of the uterine cervix with Chlamydia trachomatis, 284 women attending a venereal disease clinic were studied, were studied, of whom 58 (20.4%) yielded the organism from the cervix. Women with chlamydial cervical infection showed no distinctive symptoms.

Triple tetracycline (Deteclo) in the treatment of chlamydial infection of the female genital tract.

The efficacy of treating genital Chlamydia in women using Deteclo 300 mg twice daily for seven and 21 days has been studied. Forty-four patients were treated for seven days and 20 for 21 days. Seven days of treatment was as effective in eliminating Chlamydia trachomatis from the female genital tract as 21 days.

Isolation of Chlamydia trachomatis from women attending a clinic for sexually transmitted diseases.

Attempts were made to isolate Chlamydia trachomatis from the cervix of 300 women attending a clinic for sexually transmitted diseases in Leeds. The women were divided into four groups; (1) 130 were consorts of men suffering from non-specific urethritis; (2) 66 were suffering from gonorrhoea, or were consorts of men suffering from this disease; (3) 56 were suffering from other sexually transmitted diseases; (4) 48 had no evidence of STD. The overall isolation rate of Chlamydia trachomatis was 20%.

Diagnosis of gonorrhoea in women.

250 consecutive female contacts of men with gonorrhoea were examined in Lydia Department of Genito-urinary Medicine, St Thomas' Hospital, London, and 165 (66 per cent.) were found to be positive for gonorrhoea. Over 99 per cent.

Evaluation of cryo-stimulation test for the diagnosis of cold urticaria.

A cryo-stimulation test performed on 300 allergic and non-allergic individuals showed that this test is positive in most of the cold urticaria patients but negative in patients having other allergic or non-allergic disorders. The chief causes of false positive reactions were a state of atopy and dermographism.

Idiopathic acquired cold urticaria in North India.

Forty-three otherwise healthy patients mostly between the ages of 10 and 40 were seen in North India during a two-year period with urticaria which developed on taking a cold water bath, exposure to cold winds, evaporation of rain water or sweat in that order of frequency. There was no familial predisposition to cold urticaria or atopic disorders. The refrigeration test for cryo-proteins was negative.