Medium Optimization by Response Surface Methodology for Improved Cholesterol Oxidase Production by a Newly Isolated NAM-19 Strain.

Cholesterol oxidase is an alcohol oxidoreductase flavoprotein with wide biotechnological applications. The current work describes the isolation of a potential cholesterol oxidase producing streptomycete from Egyptian soil. The isolated strain produced cholesterol oxidase in submerged culture using a medium containing glucose, yeast extract, malt extract, and CaCO with the addition of cholesterol as an inducer.