Hepatitis B-surface antigen (HBsAg) vaccine fabricated chitosan-polyethylene glycol nanocomposite (HBsAg-CS-PEG- NC) preparation, immunogenicity, controlled release pattern, biocompatibility or non-target toxicity.

Antigen delivery framework utilising polymer-based nanomaterial is broadly used in biomedicine because of their high efficacy, antigenicity and biocompatibility. In this study, hepatitis B surface antigen fabricated chitosan-polyethylene glycol nanocomposite (HBsAg-CS-PEG NC) was prepared by in situ ionic gelation method which brought about highly stable nanoformulation that was characterised by electron microscopy, atomic force microscopy (AFM) Fourier transform infrared spectroscopy (FTIR). Particles morphology by electron microscopy studies reveals spherical, electron-dense, nanostructure with 100-120 nm.