Production and purification of mannan oligosaccharide with epithelial tight junction enhancing activity.

Background: Mannanan oligosaccharide (MOS) is well-known as effective supplement food for livestock to increase their nutrients absorption and health status, but the structure and identification of bioactive MOS remain unclear. In this study, MOS production was accomplished, using enzymatic hydrolysis of pretreated coconut meal substrate with recombinant mannanase.

Methods: The mannanase gene was cloned from AE24, then expressed in BL21.