Publications by authors named "Nausica Otero"
Article Synopsis
- This study evaluated the growth and functionality of human corneal endothelial cells (hCEC) sourced from elderly donors (>60 years) using a specific preservation method, aiming to determine their potential for clinical use despite not meeting standard criteria for corneal transplants.* -
- Cells were cultured under controlled conditions, utilizing collagenase and trypsin to isolate them, and various assays (WST-1, morphometric analysis, immunocytochemistry, TEER) were conducted to assess their growth, morphology, and functionality over time.* -
- Results showed equivalent growth profiles in hCEC from different preservation methods, maintained specific cell shapes and markers initially, but decreased in functionality after the first passage, indicating challenges in sustaining endothelial properties post-culture
Since the start of the pandemic, the tissue donation in Catalonia (Spain) has decreased drastically. At the beginning of the lockdown (from March to May 2020) there was a drop of around 70% in donation of corneas and of approximately 90% in donation of placentas. Despite the fast updating of standard operating procedures, we had big difficulties in different points.
View Article and Find Full Text PDFPurpose: To investigate the tolerability, security and long-term efficacy of lyophilized amniotic membrane (LAM) as an alternative to cryopreserved amniotic membrane in pterygium surgery.
Material And Methods: Prospective case series of patients with primary nasal pterygium who undergone pterygium surgery and LAM implant either with sutures or glue. Postoperative follow-up was until month 24.
Objectives: To evaluate microbiological culture rate and positivity of corneoscleral rim and cornea storage media as well as possible risk factors for contamination with real-world data.
Methods: Data of consecutive cornea donors implanted in the reference centre from January 2013 to January 2018 were reviewed. Information about cornea characteristics (donor demographic data, endothelial cell density, type of cornea conservation, days of storage, and precut vs full-thickness tissue), and microbiological culture information (corneoscleral rim vs storage sample, positive result) were statistically analysed.