The impact of acquired immunodeficiency in a patient with b-cell chronic lymphocytic leukemia on the course of coronavirus disease (COVID-19): a case report.

Chronic lymphocytic leukemia (CLL) is a lymphoproliferative disorder that affects the bone marrow, lymph nodes, and spleen, and is associated with certain complications. One of the complications of CLL is acquired immunodeficiency, leading to a predisposition for prolonged respiratory viral and bacterial infections. This publication presents a clinical case of a prolonged and severe course of COVID-19 in a patient with CLL, who was successfully treated with immunoglobulin replacement therapy.

PATIENTS' SAFETY: APPLICATION OF SIMULATION METHODS FOR THE TRAINING OF SPECIALISTS IN "ENT" SPECIALTY AT THE POSTGRADUATE LEVEL.

Objective: The aim: The aim of the study was to analyze the results of the practical implementation of simulation training in educational practice at the postgraduate level at Bogomolets National Medical University, majoring in "otorhinolaryngology".

Patients And Methods: Materials and methods: The study of the opinion of intern doctors regarding the acquisition of practical skills on the clinical basis of the internship was conducted at the Department of Otorhinolaryngology (Bogomolets National Medical University). The survey was conducted according to a developed questionnaire with questions on the assessment of the acquisition of competencies and practical skills in the speciality "otorhinolaryngology" during the extramural part of the internship.

CURRENT CHALLENGES FOR THE HEALTH CARE SYSTEM DUE TO THE LACK OF MEDICAL STAFF AND THE CONTINUOUS PROFESSIONAL DEVELOPMENT OF DOCTORS.

Objective: The aim: Research of the ways of adequate solving of problems with understuffed healthcare system and problems with continuous professional development of doctors.

Patients And Methods: Materials and methods: We have used a official statistical data for the period from 2009-2020 years. This data was analyzed with usage of systemic approach and statistical epidemiological analysis.

COVID-19 AS A STRESS TEST OF HEALTHCARE ESTABLISHMENTS EFFECTIVENESS AND RELIABILITY MEASURED NATIONALLY AND GLOBALLY.

Objective: The aim: The authors aimed to estimate the healthcare environment risks and safety problems of the medical staff and patients, methods of neutralizing the negative public health effects and to suggest the new approaches to improved effectiveness and reliability of the healthcare establishments functioning under the emergencies.

Patients And Methods: Materials and methods: The study includes data of questioning of 163 healthcare workers of certain institutions in Ukraine using the questionnaire of the Agency for Healthcare Research and Quality ( the USA) on adherence to the patients' safety culture. In this study only the data on the patients' safety culture "response to mistakes" are represented.

Technology for evaluating the ability of probiotic strains to accumulate copper ions and using bacteria.

Background And Aim: Microorganisms of the genus comprising probiotics could have an antitoxic effect that is manifested in the active excretion of toxic substances from the body, as well as heavy metals. This study was conducted to assess the effects of copper ions on probiotic strains based on the members of the genus and .

Materials And Methods: The following probiotic preparations were selected for this experiment: "Sporobacterin," "Bactisubtil," and "Vetom-2.

SAFETY OF PATIENTS AND MEDICAL STAFF IN CURRICULA AND TRAINING PROGRAMS FOR MEDICAL PROFESSIONALS IN UKRAINE.

Objective: The aim: The Canada and Poland experience in the educational field in the practical implementation of international practices for the prevention of patient safety incidents is significant, taking into consideration the effectiveness of health care models. We have to analyze the documents on the training of medical professionals in terms of safety as a first step in introducing into the domestic (Ukrainian) medical educational practice of existing international experience in teaching patient safety.

Patients And Methods: Materials and methods: The research was based on international documents on patient safety, thematic scientific publications, plans, and programs for the training of medical professionals in Ukraine.

Escherichia albertii EA046 (O9) harbors two polysaccharide gene clusters for synthesis of the O-antigen by the Wzx/Wzy-dependent pathway and a mannan shared by Escherichia coli O8 by the Wzm/Wzt-dependent pathway.

O antigen is a polysaccharide chain of a lipopolysaccharide on the outer membrane of Gram-negative bacteria. O-antigen-based serotyping and molecular typing are widely used for epidemiological and surveillance purposes. Two polysaccharides were isolated by Sephadex G-50 gel-permeation chromatography following mild acid degradation of the lipopolysaccharide of Escherichia albertii EA046 assigned to serotype O9.

The O-polysaccharide of Escherichia coli F5, which is structurally related to that of E. coli O28ab, provides cells only weak protection against bacteriophage attack.

Several types of Escherichia coli O-antigens form highly effective shields protecting the bacterial cell surface and preventing bacteriophages from interacting directly with their secondary (terminal) receptors. However, it is not clear if O-antigens of various types (O-serotypes) differ in their anti-phage protection efficacy. Here, we describe a new E.

Colitose-containing O-polysaccharide structure and O-antigen gene cluster of Escherichia albertii HK18069 related to those of Escherichia coli O55 and E. coli O128.

A 3,6-dideoxy-l-xylo-hexose (colitose)-containing partially O-acetylated branched polysaccharide was obtained by mild acid hydrolysis (2% HOAc, 100 °C, 2 h) of the lipopolysaccharide of Escherichia albertii HK18069 followed by gel-permeation chromatography on Sephadex G-50 Superfine. Part of colitose residues (~40%) was cleaved upon hydrolysis, and the full cleavage was achieved by prolonged hydrolysis (8 h) under the same conditions and resulted in a modified linear polysaccharide. Structure of the O-polysaccharide of E.

Erratum to: "Structural Relationships Between Genetically Closely Related O-Antigens of Escherichia coli and Shigella spp." [Biochemistry (Moscow), 81, 600 (2016)].

This corrects the article DOI: 10.1134/S0006297916060067.

Structural studies on the O-polysaccharide of Escherichia coli O57.

Mild acid hydrolysis of the lipopolysaccharide of Escherichia coli O57 afforded an O-polysaccharide, which was isolated by gel permeation chromatography (GPC) and studied by sugar analysis, Smith degradation and solvolysis with trifluoroacetic acid, along with 2D H and C NMR spectroscopy. The O-polysaccharide was found to contain d-Glc, d-Gal, d-GalA, d-GlcNAc, and l-FucNAc, as well as O-acetyl groups. Smith degradation of the O-deacetylated polysaccharide destroyed side-branch β-Glсp and α-GalpA to give a modified linear polysaccharide.

Structural and genetic relatedness of the O-antigens of Escherichia coli O50 and O2.

An O-specific polysaccharide (O-antigen) was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O50 followed by gel chromatography on Sephadex G-50. The following structure of the tetrasaccharide repeat was established by sugar analysis and 1D and 2D H and C NMR spectroscopy: →3)-α-l-Rhap-(1 → 2)-α-l-Rhap-(1 → 3)-β-l-Rhap-(1 → 4)-β-d-GlcpNAc-(1→ The linear O50 polysaccharide has the same structure as the main chain of the branched O polysaccharide of E. coli O2 studied earlier [Jansson et al.

Structure and gene cluster of the O-antigen of Escherichia coli O54.

Mild acid hydrolysis of the lipopolysaccharide of Escherichia coli O54 afforded an O-polysaccharide, which was studied by sugar analysis, solvolysis with anhydrous trifluoroacetic acid, and H and C NMR spectroscopy. Solvolysis cleaved predominantly the linkage of β-d-Ribf and, to a lesser extent, that of β-d-GlcpNAc, whereas the other linkages, including the linkage of α-l-Rhap, were stable under selected conditions (40 °C, 5 h). The following structure of the O-polysaccharide was established: →4)-α-d-GalpA-(1 → 2)-α-l-Rhap-(1 → 2)-β-d-Ribf-(1 → 4)-β-d-Galp-(1 → 3)-β-d-GlcpNAc-(1→ The O-antigen gene cluster of E.

Studies on the O-polysaccharide of Escherichia albertii O2 characterized by non-stoichiometric O-acetylation and non-stoichiometric side-chain l-fucosylation.

An O-polysaccharide was isolated from the lipopolysaccharide of Escherichia albertii O2 and studied by chemical methods and 1D and 2D H and C NMR spectroscopy. The following structure of the O-polysaccharide was established: . The O-polysaccharide is characterized by masked regularity owing to a non-stoichiometric O-acetylation of an l-fucose residue in the main chain and a non-stoichiometric side-chain l-fucosylation of a β-GlcNAc residue.

Structure and genetics of a glycerol 2-phosphate-containing O-specific polysaccharide of Escherichia coli O33.

An O-specific polysaccharide was isolated by mild acid degradation of the lipopolysaccharide of Escherichia coli O33 followed by gel-permeation chromatography on Sephadex G-50. The polysaccharide was found to contain glycerol 2-phosphate (Gro-2-P), and the following structure of its tetrasaccharide repeat was established by sugar analysis, dephosphorylation, and 1D and 2D H and C NMR spectroscopy: The O33-antigen gene cluster was analyzed and found to be essentially consistent with the O-polysaccharide structure.

Structure elucidation of the O-specific polysaccharide by NMR spectroscopy and selective cleavage and genetic characterization of the O-antigen of Escherichia albertii O5.

The O-specific polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Escherichia albertii O5 (strain T150248) and studied by sugar analysis, selective cleavages of glycosidic linkages, and 1D and 2D H and C NMR spectroscopy. Partial solvolysis with anh (anhydrous) CFCOH and hydrolysis with 0.05 M CFCOH cleaved predominantly the glycosidic linkage of β-GalpNAc or β-Galf, respectively, whereas the linkages of α-GlcpNAc and β-Galp were stable.

Structures and gene clusters of the O-antigens of Escherichia albertii O3, O4, O6, and O7.

The O-specific polysaccharides (OPSs) called O-antigens were obtained by mild acid degradation of the lipopolysaccharides of Escherichia albertii serotypes O3, O4, O6, and O7 and studied by sugar analysis along with 1D and 2D H and C NMR spectroscopy. The following structure was established for the OPS of E. albertii O4, which, to our knowledge, is unique among known bacterial polysaccharide structures: →2)-α-l-Rhap-(1 → 2)-α-l-Fucp-(1 → 2)-β-d-Galp-(1 → 3)-α-d-GalpNAc-(1 → 3)-β-d-GlcpNAc-(1→ The OPS structure of the strain of E.

Structure and gene cluster of the O-antigen of Escherichia albertii O1 resembling the O-antigen of Pseudomonas aeruginosa O5.

The O-specific polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Escherichia albertii serotype O1 strain SP20140089 and studied by sugar analysis along with 1D and 2D H and C NMR spectroscopy. The following structure was established for the trisaccharide repeating unit of the O-polysaccharide: →4)-β-d-ManpNAc3NAcA-(1 → 4)-β-d-GlcpNAm3NAcA-(1 → 3)-α-d-GlcpNAc-(1→ where ManNAc3NAcA and GlcNAm3NAcA indicate 2,3-diacetamido-2,3-dideoxymannuronic acid and 2-acetimidoylamino-3-acetamido-2,3-dideoxyglucuronic acid, respectively. While showing some similarity with O-polysaccharide structures of a group of Pseudomonas aeruginosa serotypes (O2, O5, O16, O18, and O20), that of E.

Structure and genetics of the O-antigens of Escherichia coli O182-O187.

O-polysaccharides (OPSs) were obtained by mild acid degradation of the lipopolysaccharides of Escherichia coli O182-O187, and their structures were established by sugar analysis, Smith degradation, and H and C NMR spectroscopy. In addition to the monosaccharides that occur often in E. coli OPSs (d-Glc, d-Gal, d-Man, d-GlcNAc, d-GalNAc, d-GlcA, l-Fuc, d-Rib), a number of less common components were identified as the OPS constituents, including 2-acetamido-2-deoxy-l-quinovose and 4-deoxy-4-[(S)-3-hydroxybutanoyl-l-alanyl]-d-quinovose (O186), 3-acetamido-3-deoxy-d-fucose (O187), 3-deoxy-3-[(R)-3-hydroxybutanoyl]-d-fucose (O184), and 2,3-diacetamido-2,3-dideoxy-l-rhamnose (O182).

Structural Relationships Between Genetically Closely Related O-Antigens of Escherichia coli and Shigella spp.

Gene clusters for biosynthesis of 24 of 34 basic O-antigen forms of Shigella spp. are identical or similar to those of the genetically closely related bacterium Escherichia coli. For 18 of these relatedness was confirmed chemically by elucidation of the O-antigen (O-polysaccharide) structures.

Perception of straightness and parallelism with minimal distance information.

The ability of human observers to judge the straightness and parallelism of extended lines has been a neglected topic of study since von Helmholtz's initial observations 150 years ago. He showed that there were significant misperceptions of the straightness of extended lines seen in the peripheral visual field. The present study focused on the perception of extended lines (spanning 90° visual angle) that were directly fixated in the visual environment of a planetarium where there was only minimal information about the distance to the lines.

[COMPARISON OF THE OCCURRENCE RATE OF NAUSEA AND VOMITING AFTER TRANSCUTANEOUS NEPHROLITHOTRIPSY: SEVOFLURANE OR PROPOFOL].

Impact of various kinds of general anesthesia while performing transcutaneous endoscopic interventions on kidneys on postoperative occurrence and rate of nausea and vomiting were studied. Propofol and sevofluran were used for support of general anesthesia conduction. There was established, that if optimal depth of anesthesia is maintained (bispectral index 40 - 60 units), the rate of nausea and vomiting occurrence do not depend on the kind of anesthesia applied--total intravenous or the inhalation one.

The new moon illusion and the role of perspective in the perception of straight and parallel lines.

In the new moon illusion, the sun does not appear to be in a direction perpendicular to the boundary between the lit and dark sides of the moon, and aircraft jet trails appear to follow curved paths across the sky. In both cases, lines that are physically straight and parallel to the horizon appear to be curved. These observations prompted us to investigate the neglected question of how we are able to judge the straightness and parallelism of extended lines.

Potential energy surface of HDO up to 25,000 cm-1.

A new spectroscopically determined potential energy surface (PES) for HD(16)O is presented. This surface is constructed by adjusting the high accuracy ab initio PES of Polyansky et al. [Science 299, 539 (2003)] by fitting to both published experimental data and our still unpublished data.