Publications by authors named "Natsumi Iki"

CRISPR-Cas technology has enabled the rapid and effortless generation of genetically modified mice. Specifically, mice and point mutant mice are readily produced by electroporation of CRISPR factors (and single-stranded oligo DNA donors) into the zygote. In contrast, gene cassette (>1 kb) knock-in and floxed mice are mainly generated by microinjection of CRISPR factors and double-stranded DNA donors into zygotes.

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Genome editing can introduce designed mutations into a target genomic site. Recent research has revealed that it can also induce various unintended events such as structural variations, small indels, and substitutions at, and in some cases, away from the target site. These rearrangements may result in confounding phenotypes in biomedical research samples and cause a concern in clinical or agricultural applications.

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Proximity-dependent biotin identification (BioID) is a useful method to identify unknown protein-protein interactions. Few reports have described genetically engineered knock-in mouse models for in vivo BioID. Thus, little is known about the proper method for biotin administration and which tissues are applicable.

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Article Synopsis
  • Genetically modified mice are crucial for studying gene function and human diseases, and genome editing tools like CRISPR-Cas allow for specific mutations in embryos.
  • The study introduces a modified version of the Cas9 protein (Cas9-mC) linked to the Cdt1 protein to enhance the efficiency of creating genetically engineered mice.
  • Cas9-mC showed higher success rates in producing critical genetic alterations, including full gene deletions and knock-in mutations, compared to traditional Cas9 methods, making it a valuable resource for researchers.
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