Publications by authors named "Natsumi Himeno"
Article Synopsis
- Protein-protein interactions (PPIs) are crucial for many biological functions, and understanding them is essential for studying how proteins work.
- The study proposes a straightforward method to assess PPI using mammalian cells, involving introduction of expression vectors, cell lysis, and protein isolation with an affinity gel.
- The researchers also suggest using affinity antibodies to confirm PPIs, highlighting the importance of maintaining protein integrity throughout the process for accurate results.
Residual pancreatic endocrine function is important for maintaining metabolic status after pancreatectomy and is closely related to patient nutritional status and prognosis. In contrast to insulin secretion, the significance of glucagon secretion following pancreatectomy remains unclear. In this study, we assessed the changes in pancreatic glucagon secretion during pancreatectomy to determine their pathophysiological significance.
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- * Long-term use of stimulants to maintain their function can have undesirable side effects, making PPARα a better target for preserving beige adipocytes.
- * Pemafibrate, a medication used for treating dyslipidemia, has been shown to enhance the thermogenic ability of these cells, reduce body weight gain, and improve glucose tolerance in obese mouse models.
Fulminant type 1 diabetes mellitus (FT1DM) is a subtype of type 1 diabetes mellitus and is characterized by a remarkably abrupt onset and almost complete destruction of β-cells within a few days. Here, we report a case of diabetic ketoacidosis in a 63-year-old man with no history of hyperglycemia. The patient was diagnosed with FT1DM and had almost no insulin secretion.
View Article and Find Full Text PDFIdentification of proteins that bind to the neuroprotective agent neoechinulin A.
Biosci Biotechnol Biochem
March 2018
Neoechinulin A is an indole alkaloid with several biological activities. We previously reported that this compound protects neuronal PC12 cells from cytotoxicity induced by the peroxynitrite generator 3-morpholinosydnonimine (SIN-1), but the target proteins and precise mechanism of action of neoechinulin A were unclear. Here, we employed a phage display screen to identify proteins that bind directly with neoechinulin A.
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- - The study describes the first successful total synthesis of MA026, a unique lipocyclodepsipeptide found in Pseudomonas sp. RtIB026, and identifies its potential target protein for combating hepatitis C virus (HCV).
- - MA026 is composed of a cyclodepsipeptide, chain peptide, and N-terminal (R)-3-hydroxydecanoic acid, with its synthesis involving intricate steps like calorie coupling and macrocyclization to create its active form.
- - Testing in HCV cell cultures shows that MA026 effectively reduces HCV infection by interfering with the virus's entry into liver cells, and further analysis identified claudin-1 as its specific target.