Publications by authors named "Nathaniel B Axtell"
Easy and Accessible Workflow for Label-Free Single-Cell Proteomics.
J Am Soc Mass Spectrom
October 2023
Article Synopsis
- Single-cell proteomics (SCP) offers unique insights unattainable through bulk measurements or other omics, but its accessibility is limited due to the need for custom equipment and expertise.
- A new low-cost HP D100 Single Cell Dispenser was tested and found to accurately isolate single cells for SCP, achieving around 97% effective proteome coverage in 384-well plates.
- This new workflow, combined with commercial well plates, provides an affordable and efficient alternative for SCP, making it more accessible for a wider range of laboratories.
Formalin-fixed, paraffin-embedded (FFPE) tissues are banked in large repositories to cost-effectively preserve valuable specimens for later study. With the rapid growth of spatial proteomics, FFPE tissues can serve as a more accessible alternative to more commonly used frozen tissues. However, extracting proteins from FFPE tissues is challenging due to cross-links formed between proteins and formaldehyde.
View Article and Find Full Text PDFRecent advances in sample preparation and analysis have enabled direct profiling of protein expression in single mammalian cells and other trace samples. Several techniques to prepare and analyze low-input samples employ custom fluidics for nanoliter sample processing and manual sample injection onto a specialized separation column. While being effective, these highly specialized systems require significant expertise to fabricate and operate, which has greatly limited implementation in most proteomic laboratories.
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